Effect of different vitamin D3 metabolites on intestinal calcium homeostasis-related gene expression in broiler chickens
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Zootecnia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100511 |
Resumo: | ABSTRACT The purpose of this study was to investigate the effects of vitamin D3 metabolites 1α-hydroxycholecalciferol (1α(OH)D3), 25-hydroxycholecalciferol (25(OH)2D3), and 1,25-dihydroxycholecalciferol (1,25(OH)2D3) on growth performance, bone quality, and intestinal calcium homeostasis-related gene expression in broiler chickens. One-day-old broilers were fed a basal diet and basal diet containing different vitamin D3 metabolites. The body weight, feed intake, and feed conversion ratio in control and experimental broilers were measured to assess the growth performance, mineral levels, and bone breaking strength. The duodenum was used to assess calcium homeostasis-related gene expressions by quantitative reverse transcription-PCR. No statistically significant difference was found in growth performance, mineral deposition, or bone breaking strength in broiler chickens after three weeks feeding with vitamin D3. However, supplementation of vitamin D3 metabolites tended to improve feed conversion rate, bone mineral deposition, and breaking strength in broiler chickens. The results demonstrated that vitamin D3 metabolites significantly upregulated calcium homeostasis-related genes, including calbindin, β-glucuronidase, TRPV6, and Na/Pi IIb cotransporter, mRNA levels after 12 h of feeding. The vitamin D3 metabolite 1,25(OH)2D3 was the most effective at regulating calcium homeostasis-associated gene expression after 6 h of feeding. Dietary vitamin D3 metabolites may alleviate the development of TD in broiler chickens and these effects probably occur through regulation of intestinal calcium homeostasis-related gene expression. |
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Revista Brasileira de Zootecnia (Online) |
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Effect of different vitamin D3 metabolites on intestinal calcium homeostasis-related gene expression in broiler chickensgene1α-hydroxycholecalciferol25-hydroxycholecalciferol1,25-dihydroxycholecalciferolABSTRACT The purpose of this study was to investigate the effects of vitamin D3 metabolites 1α-hydroxycholecalciferol (1α(OH)D3), 25-hydroxycholecalciferol (25(OH)2D3), and 1,25-dihydroxycholecalciferol (1,25(OH)2D3) on growth performance, bone quality, and intestinal calcium homeostasis-related gene expression in broiler chickens. One-day-old broilers were fed a basal diet and basal diet containing different vitamin D3 metabolites. The body weight, feed intake, and feed conversion ratio in control and experimental broilers were measured to assess the growth performance, mineral levels, and bone breaking strength. The duodenum was used to assess calcium homeostasis-related gene expressions by quantitative reverse transcription-PCR. No statistically significant difference was found in growth performance, mineral deposition, or bone breaking strength in broiler chickens after three weeks feeding with vitamin D3. However, supplementation of vitamin D3 metabolites tended to improve feed conversion rate, bone mineral deposition, and breaking strength in broiler chickens. The results demonstrated that vitamin D3 metabolites significantly upregulated calcium homeostasis-related genes, including calbindin, β-glucuronidase, TRPV6, and Na/Pi IIb cotransporter, mRNA levels after 12 h of feeding. The vitamin D3 metabolite 1,25(OH)2D3 was the most effective at regulating calcium homeostasis-associated gene expression after 6 h of feeding. Dietary vitamin D3 metabolites may alleviate the development of TD in broiler chickens and these effects probably occur through regulation of intestinal calcium homeostasis-related gene expression.Sociedade Brasileira de Zootecnia2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100511Revista Brasileira de Zootecnia v.47 2018reponame:Revista Brasileira de Zootecnia (Online)instname:Sociedade Brasileira de Zootecnia (SBZ)instacron:SBZ10.1590/rbz4720170015info:eu-repo/semantics/openAccessHsiao,Felix Shih-HsiangCheng,Yeong-HsiangHan,Jin-ChengChang,Ming-HuangYu,Yu-Hsiangeng2018-05-18T00:00:00Zoai:scielo:S1516-35982018000100511Revistahttps://www.rbz.org.br/pt-br/https://old.scielo.br/oai/scielo-oai.php||bz@sbz.org.br|| secretariarbz@sbz.org.br1806-92901516-3598opendoar:2018-05-18T00:00Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ)false |
dc.title.none.fl_str_mv |
Effect of different vitamin D3 metabolites on intestinal calcium homeostasis-related gene expression in broiler chickens |
title |
Effect of different vitamin D3 metabolites on intestinal calcium homeostasis-related gene expression in broiler chickens |
spellingShingle |
Effect of different vitamin D3 metabolites on intestinal calcium homeostasis-related gene expression in broiler chickens Hsiao,Felix Shih-Hsiang gene 1α-hydroxycholecalciferol 25-hydroxycholecalciferol 1,25-dihydroxycholecalciferol |
title_short |
Effect of different vitamin D3 metabolites on intestinal calcium homeostasis-related gene expression in broiler chickens |
title_full |
Effect of different vitamin D3 metabolites on intestinal calcium homeostasis-related gene expression in broiler chickens |
title_fullStr |
Effect of different vitamin D3 metabolites on intestinal calcium homeostasis-related gene expression in broiler chickens |
title_full_unstemmed |
Effect of different vitamin D3 metabolites on intestinal calcium homeostasis-related gene expression in broiler chickens |
title_sort |
Effect of different vitamin D3 metabolites on intestinal calcium homeostasis-related gene expression in broiler chickens |
author |
Hsiao,Felix Shih-Hsiang |
author_facet |
Hsiao,Felix Shih-Hsiang Cheng,Yeong-Hsiang Han,Jin-Cheng Chang,Ming-Huang Yu,Yu-Hsiang |
author_role |
author |
author2 |
Cheng,Yeong-Hsiang Han,Jin-Cheng Chang,Ming-Huang Yu,Yu-Hsiang |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Hsiao,Felix Shih-Hsiang Cheng,Yeong-Hsiang Han,Jin-Cheng Chang,Ming-Huang Yu,Yu-Hsiang |
dc.subject.por.fl_str_mv |
gene 1α-hydroxycholecalciferol 25-hydroxycholecalciferol 1,25-dihydroxycholecalciferol |
topic |
gene 1α-hydroxycholecalciferol 25-hydroxycholecalciferol 1,25-dihydroxycholecalciferol |
description |
ABSTRACT The purpose of this study was to investigate the effects of vitamin D3 metabolites 1α-hydroxycholecalciferol (1α(OH)D3), 25-hydroxycholecalciferol (25(OH)2D3), and 1,25-dihydroxycholecalciferol (1,25(OH)2D3) on growth performance, bone quality, and intestinal calcium homeostasis-related gene expression in broiler chickens. One-day-old broilers were fed a basal diet and basal diet containing different vitamin D3 metabolites. The body weight, feed intake, and feed conversion ratio in control and experimental broilers were measured to assess the growth performance, mineral levels, and bone breaking strength. The duodenum was used to assess calcium homeostasis-related gene expressions by quantitative reverse transcription-PCR. No statistically significant difference was found in growth performance, mineral deposition, or bone breaking strength in broiler chickens after three weeks feeding with vitamin D3. However, supplementation of vitamin D3 metabolites tended to improve feed conversion rate, bone mineral deposition, and breaking strength in broiler chickens. The results demonstrated that vitamin D3 metabolites significantly upregulated calcium homeostasis-related genes, including calbindin, β-glucuronidase, TRPV6, and Na/Pi IIb cotransporter, mRNA levels after 12 h of feeding. The vitamin D3 metabolite 1,25(OH)2D3 was the most effective at regulating calcium homeostasis-associated gene expression after 6 h of feeding. Dietary vitamin D3 metabolites may alleviate the development of TD in broiler chickens and these effects probably occur through regulation of intestinal calcium homeostasis-related gene expression. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100511 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100511 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/rbz4720170015 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Zootecnia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Zootecnia |
dc.source.none.fl_str_mv |
Revista Brasileira de Zootecnia v.47 2018 reponame:Revista Brasileira de Zootecnia (Online) instname:Sociedade Brasileira de Zootecnia (SBZ) instacron:SBZ |
instname_str |
Sociedade Brasileira de Zootecnia (SBZ) |
instacron_str |
SBZ |
institution |
SBZ |
reponame_str |
Revista Brasileira de Zootecnia (Online) |
collection |
Revista Brasileira de Zootecnia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ) |
repository.mail.fl_str_mv |
||bz@sbz.org.br|| secretariarbz@sbz.org.br |
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1750318152775892992 |