Unexplored Brazilian oceanic Island host high salt tolerant biosurfactant-producing bacterial strains

• Autor(a) principal: Silva, Fábio Sérgio Paulino da
• Data de Publicação: 2015
• Outros Autores: Pylro, Victor Satler, Fernandes, Pericles Leonardo, Barcelos, Gisele Souza, Kalks, Karlos Henrique Martins, Schaefer, Carlos Ernesto Gonçalves Reynaud, Tótola, Marcos Rogério
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: https://doi.org/10.1007/s00792-015-0740-7; http://www.locus.ufv.br/handle/123456789/19762
• topic: Trindade island; Oceanic islands; Biosurfactants; Bacillus

We aimed to isolate biosurfactant-producing bacteria in high salt conditions from uncontaminated soils on the Brazilian oceanic island, Trindade. Blood agar medium was used for the isolation of presumptive biosurfactant-producing bacteria. Confirmation and measurements of biosurfactant production were made using an oil-spreading method. The isolates were identified by fatty acid profiles and partial 16S rRNA gene sequence analysis. A total of 14 isolates obtained from the 12 soil samples were found to produce biosurfactants. Among them, two isolates stood out as being able to produce biosurfactant that is increasingly active in solutions containing up to 175 g L−1 NaCl. These high salt tolerant biosurfactant producers are affiliated to different species of the genus Bacillus. Soil organic matter showed positive correlation with the number of biosurfactant-producing bacteria isolated from our different sampling sites. The applied approach successfully recovered and identified biosurfactant-producing bacteria from non-contaminated soils. Due to the elevated salt tolerance, as well as their capacity to produce biosurfactants, these isolates are promising for environmental biotechnological applications, especially in the oil production chain.