Genetic diversity and some aspects of antimicrobial activity of lactic acid bacteria isolated from goat milk
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1007/s12010-015-1511-8 http://www.locus.ufv.br/handle/123456789/19406 |
Resumo: | Lactic acid bacteria (LAB, n = 57) were previously obtained from raw goat milk, identified as Lactococcus spp. (n = 24) and Enterococcus spp. (n = 33), and characterized as bacteriocinogenic. Fingerprinting by pulsed field gel electrophoresis (PFGE) demonstrated high genetic diversity, and 30 strains were selected and exhibited strong antimicrobial activity against 46 target strains (LAB, spoilage, and foodborne pathogens). Six strains (Lactococcus lactis: GLc03 and GLc05; and Enterococcus durans: GEn09, GEn12, GEn14, and GEn17) were selected to characterize their bacteriocinogenic features, using Listeria monocytogenes ATCC 7644 as the target. The six strains produced bacteriocins at higher titer when incubated in MRS at 37 °C up to 12 h, when compared to growth at 25 and 30 °C. The produced bacteriocins kept their antimicrobial activity after exposure to 100 °C for 2 h and 121 °C for 20 min; the antimicrobial activity was also observed after treatment at pH 2.0 to 10.0, except for GLc03. L. monocytogenes populations were reduced approximately two logs after treatment with cell-free supernatants from the selected strains. These data show that goat milk can contain a diverse microbiota able to inhibit L. monocytogenes, a common pathogen found in dairy products, and can be potentially employed in biopreservation of food produced under different processing conditions. |
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2145 |
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Genetic diversity and some aspects of antimicrobial activity of lactic acid bacteria isolated from goat milkGoat milkLactic acid bacteriaBacteriocinsPFGEInhibitionListeria monocytogenesLactic acid bacteria (LAB, n = 57) were previously obtained from raw goat milk, identified as Lactococcus spp. (n = 24) and Enterococcus spp. (n = 33), and characterized as bacteriocinogenic. Fingerprinting by pulsed field gel electrophoresis (PFGE) demonstrated high genetic diversity, and 30 strains were selected and exhibited strong antimicrobial activity against 46 target strains (LAB, spoilage, and foodborne pathogens). Six strains (Lactococcus lactis: GLc03 and GLc05; and Enterococcus durans: GEn09, GEn12, GEn14, and GEn17) were selected to characterize their bacteriocinogenic features, using Listeria monocytogenes ATCC 7644 as the target. The six strains produced bacteriocins at higher titer when incubated in MRS at 37 °C up to 12 h, when compared to growth at 25 and 30 °C. The produced bacteriocins kept their antimicrobial activity after exposure to 100 °C for 2 h and 121 °C for 20 min; the antimicrobial activity was also observed after treatment at pH 2.0 to 10.0, except for GLc03. L. monocytogenes populations were reduced approximately two logs after treatment with cell-free supernatants from the selected strains. These data show that goat milk can contain a diverse microbiota able to inhibit L. monocytogenes, a common pathogen found in dairy products, and can be potentially employed in biopreservation of food produced under different processing conditions.Applied Biochemistry and Biotechnology2018-05-09T11:33:36Z2018-05-09T11:33:36Z2015-01-31info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf15590291https://doi.org/10.1007/s12010-015-1511-8http://www.locus.ufv.br/handle/123456789/19406engv. 175, Issue 6, p. 2806–2822, March 2015Springer Science+Business Media New Yorkinfo:eu-repo/semantics/openAccessCavicchioli, Valéria QuintanaDornellas, Wesley dos SantosPerin, Luana MartinsPieri, Fábio AlessandroFranco, Bernadette Dora Gombossy de MeloTodorov, Svetoslav DimitrovNero, Luís Augustoreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T06:58:27Zoai:locus.ufv.br:123456789/19406Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T06:58:27LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.none.fl_str_mv |
Genetic diversity and some aspects of antimicrobial activity of lactic acid bacteria isolated from goat milk |
title |
Genetic diversity and some aspects of antimicrobial activity of lactic acid bacteria isolated from goat milk |
spellingShingle |
Genetic diversity and some aspects of antimicrobial activity of lactic acid bacteria isolated from goat milk Cavicchioli, Valéria Quintana Goat milk Lactic acid bacteria Bacteriocins PFGE Inhibition Listeria monocytogenes |
title_short |
Genetic diversity and some aspects of antimicrobial activity of lactic acid bacteria isolated from goat milk |
title_full |
Genetic diversity and some aspects of antimicrobial activity of lactic acid bacteria isolated from goat milk |
title_fullStr |
Genetic diversity and some aspects of antimicrobial activity of lactic acid bacteria isolated from goat milk |
title_full_unstemmed |
Genetic diversity and some aspects of antimicrobial activity of lactic acid bacteria isolated from goat milk |
title_sort |
Genetic diversity and some aspects of antimicrobial activity of lactic acid bacteria isolated from goat milk |
author |
Cavicchioli, Valéria Quintana |
author_facet |
Cavicchioli, Valéria Quintana Dornellas, Wesley dos Santos Perin, Luana Martins Pieri, Fábio Alessandro Franco, Bernadette Dora Gombossy de Melo Todorov, Svetoslav Dimitrov Nero, Luís Augusto |
author_role |
author |
author2 |
Dornellas, Wesley dos Santos Perin, Luana Martins Pieri, Fábio Alessandro Franco, Bernadette Dora Gombossy de Melo Todorov, Svetoslav Dimitrov Nero, Luís Augusto |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Cavicchioli, Valéria Quintana Dornellas, Wesley dos Santos Perin, Luana Martins Pieri, Fábio Alessandro Franco, Bernadette Dora Gombossy de Melo Todorov, Svetoslav Dimitrov Nero, Luís Augusto |
dc.subject.por.fl_str_mv |
Goat milk Lactic acid bacteria Bacteriocins PFGE Inhibition Listeria monocytogenes |
topic |
Goat milk Lactic acid bacteria Bacteriocins PFGE Inhibition Listeria monocytogenes |
description |
Lactic acid bacteria (LAB, n = 57) were previously obtained from raw goat milk, identified as Lactococcus spp. (n = 24) and Enterococcus spp. (n = 33), and characterized as bacteriocinogenic. Fingerprinting by pulsed field gel electrophoresis (PFGE) demonstrated high genetic diversity, and 30 strains were selected and exhibited strong antimicrobial activity against 46 target strains (LAB, spoilage, and foodborne pathogens). Six strains (Lactococcus lactis: GLc03 and GLc05; and Enterococcus durans: GEn09, GEn12, GEn14, and GEn17) were selected to characterize their bacteriocinogenic features, using Listeria monocytogenes ATCC 7644 as the target. The six strains produced bacteriocins at higher titer when incubated in MRS at 37 °C up to 12 h, when compared to growth at 25 and 30 °C. The produced bacteriocins kept their antimicrobial activity after exposure to 100 °C for 2 h and 121 °C for 20 min; the antimicrobial activity was also observed after treatment at pH 2.0 to 10.0, except for GLc03. L. monocytogenes populations were reduced approximately two logs after treatment with cell-free supernatants from the selected strains. These data show that goat milk can contain a diverse microbiota able to inhibit L. monocytogenes, a common pathogen found in dairy products, and can be potentially employed in biopreservation of food produced under different processing conditions. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-31 2018-05-09T11:33:36Z 2018-05-09T11:33:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
15590291 https://doi.org/10.1007/s12010-015-1511-8 http://www.locus.ufv.br/handle/123456789/19406 |
identifier_str_mv |
15590291 |
url |
https://doi.org/10.1007/s12010-015-1511-8 http://www.locus.ufv.br/handle/123456789/19406 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
v. 175, Issue 6, p. 2806–2822, March 2015 |
dc.rights.driver.fl_str_mv |
Springer Science+Business Media New York info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Springer Science+Business Media New York |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
pdf application/pdf |
dc.publisher.none.fl_str_mv |
Applied Biochemistry and Biotechnology |
publisher.none.fl_str_mv |
Applied Biochemistry and Biotechnology |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1822610592176799744 |