Efeito da infusão dos frutos de Momordica charantia L. em ratas diabéticas

• Autor(a) principal: Ferreira, Ronaldo Martins
• Data de Publicação: 2008
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/290
• topic: Momordica charantia; Ratas diabéticas; Momordica charantia; Diabetic female rats; CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::NUTRICAO E ALIMENTACAO ANIMAL

The objective of this study was to characterize the clinical alterations on laboratory female rats with alloxanic diabetes and on healthy female rats, treated with aqueous infusion of powdered fruits of Momordica charantia. In the biological assays, 28 female rats were induced to the experimental diabetes though intraperitoneal injection in a dose of 75 mg/kg.. The glycemia was verified on the sixth day after the induction and the animal with glycemia superior to 180 mg/dL were distributed in two groups: Treated Diabetic Group (8 animals) and Control Diabetic Group (7 animals). After dividing this two groups of female rats (healthy and diabetic rats), two others groups of healthy animals were formed: Control Health Group and Treated Healthy Group, which one with 7 animals. Treated animals were received a diary dose of 1 mL/250g of corporal weigh of the 10 % prepared infusion, and the groups-control animals, 1mL of physiologic serum, for 25 days. During the experiment were studied the following clinical parameters: weigh, hydric ingestion, alimentary ingestion, diuresis and general state. On the final of the experiment, the animals were euthanized and the following laboratorial dosages were realized: casual glicemy, hemoglobin, A1c ,insulin, C peptide and glucose-dependent insulinotropic polypeptide (GIP). Was verified weight increase among all animals of all groups and a bigger hydric and alimentary consumption in the groups of diabetic animals. After the treatment with aqueous infusion of powdered fruits of Momordica charantia, the glycemic level of treated diabetic animals was reduced to 12,4% and the treated healthy animals was reduced to 0,38%. The found values to hemoglobin A1c of diabetic animals were similar to values that are considered normal. Plasmatic concentrations to the insulin were smaller in diabetic female rats than in healthy female rats. Sanguine tenors of the C peptide of diabetic animals showed values a little smaller than healthy animals and plasmatic concentration of the glucose-dependent insulinotropic polypeptide (GIP) were shown 101,3% larger in treated diabetic animals than in no-treated diabetic animals and 68,0% above of the found values for animals of healthy control group. These results reveal that the aqueous infusion of powdered fruits of Momordica charantia is capable to promote and increase plasmatic levels of the glucose- dependent insulinotropic polypeptide, promoting a decrease of glycemic levels of animals with alloxanic diabetes.