The Híbrido de Timor germplasm: identification of molecular diversity and resistance sources to coffee berry disease and leaf rust
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1007/s10681-018-2231-2 http://www.locus.ufv.br/handle/123456789/21783 |
Resumo: | The main phytosanitary problems affecting global coffee production are the fungal diseases known as rust, caused by Hemileia vastatrix Berkeley and Broome, and coffee berry disease (CBD), induced by Colletotrichum kahawae Waller and Bridge. The main disease control strategy is the use of resistant coffee cultivars. Híbrido de Timor is the most important source of resistant varieties used in breeding programs worldwide. The objective of this work was to characterize the diversity and disease resistance of 152 HdT genotypes from the germplasm collection at the Universidade Federal de Viçosa (UFV). Accessions were phenotyped with H. vastatrix races II and XXXIII. Molecular analysis was carried out with 29 random microsatellite markers or single sequence repeats (SSRs), and two SSRs associated with the CBD resistance gene Ck-1. All accessions in the germplasm collection were resistant to H. vastatrix race II, and 141 were resistant to H. vastatrix race XXXIII. Based on the presence of markers, there were 106 accessions containing the CBD resistance gene Ck-1. In the diversity study, the 152 accessions clustered into 21 different groups. A unique molecular profile (fingerprint) was determined for each individual, using 52 alleles from 22 SSR markers. The HdT germplasm of UFV was highly diverse, and included 99 accessions with multiple disease resistance genes, including the CBD resistance gene Ck-1, and others conferring resistance to H. vastatrix races II and XXXIII. |
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Silva, R. A.Zambolim, L.Castro, I. S. L.Rodrigues, H. S.Cruz, C. D.Caixeta, E. T.2018-09-12T17:03:01Z2018-09-12T17:03:01Z2018-08-071573-5060https://doi.org/10.1007/s10681-018-2231-2http://www.locus.ufv.br/handle/123456789/21783The main phytosanitary problems affecting global coffee production are the fungal diseases known as rust, caused by Hemileia vastatrix Berkeley and Broome, and coffee berry disease (CBD), induced by Colletotrichum kahawae Waller and Bridge. The main disease control strategy is the use of resistant coffee cultivars. Híbrido de Timor is the most important source of resistant varieties used in breeding programs worldwide. The objective of this work was to characterize the diversity and disease resistance of 152 HdT genotypes from the germplasm collection at the Universidade Federal de Viçosa (UFV). Accessions were phenotyped with H. vastatrix races II and XXXIII. Molecular analysis was carried out with 29 random microsatellite markers or single sequence repeats (SSRs), and two SSRs associated with the CBD resistance gene Ck-1. All accessions in the germplasm collection were resistant to H. vastatrix race II, and 141 were resistant to H. vastatrix race XXXIII. Based on the presence of markers, there were 106 accessions containing the CBD resistance gene Ck-1. In the diversity study, the 152 accessions clustered into 21 different groups. A unique molecular profile (fingerprint) was determined for each individual, using 52 alleles from 22 SSR markers. The HdT germplasm of UFV was highly diverse, and included 99 accessions with multiple disease resistance genes, including the CBD resistance gene Ck-1, and others conferring resistance to H. vastatrix races II and XXXIII.engEuphyticaVolumes 214, Issues 9, September 2018Springer Netherlandsinfo:eu-repo/semantics/openAccessCoffee breedingColletotrichum kahawaeDurable resistanceFingerprintingHemileia vastatrixThe Híbrido de Timor germplasm: identification of molecular diversity and resistance sources to coffee berry disease and leaf rustinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf1603286https://locus.ufv.br//bitstream/123456789/21783/1/artigo.pdfd12df30b04d943d4fb20e8c9c2baffdaMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/21783/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg5234https://locus.ufv.br//bitstream/123456789/21783/3/artigo.pdf.jpgcb5c202bd2c635ece14a0880668b4f3dMD53123456789/217832018-09-12 23:00:43.217oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-09-13T02:00:43LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
The Híbrido de Timor germplasm: identification of molecular diversity and resistance sources to coffee berry disease and leaf rust |
title |
The Híbrido de Timor germplasm: identification of molecular diversity and resistance sources to coffee berry disease and leaf rust |
spellingShingle |
The Híbrido de Timor germplasm: identification of molecular diversity and resistance sources to coffee berry disease and leaf rust Silva, R. A. Coffee breeding Colletotrichum kahawae Durable resistance Fingerprinting Hemileia vastatrix |
title_short |
The Híbrido de Timor germplasm: identification of molecular diversity and resistance sources to coffee berry disease and leaf rust |
title_full |
The Híbrido de Timor germplasm: identification of molecular diversity and resistance sources to coffee berry disease and leaf rust |
title_fullStr |
The Híbrido de Timor germplasm: identification of molecular diversity and resistance sources to coffee berry disease and leaf rust |
title_full_unstemmed |
The Híbrido de Timor germplasm: identification of molecular diversity and resistance sources to coffee berry disease and leaf rust |
title_sort |
The Híbrido de Timor germplasm: identification of molecular diversity and resistance sources to coffee berry disease and leaf rust |
author |
Silva, R. A. |
author_facet |
Silva, R. A. Zambolim, L. Castro, I. S. L. Rodrigues, H. S. Cruz, C. D. Caixeta, E. T. |
author_role |
author |
author2 |
Zambolim, L. Castro, I. S. L. Rodrigues, H. S. Cruz, C. D. Caixeta, E. T. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Silva, R. A. Zambolim, L. Castro, I. S. L. Rodrigues, H. S. Cruz, C. D. Caixeta, E. T. |
dc.subject.pt-BR.fl_str_mv |
Coffee breeding Colletotrichum kahawae Durable resistance Fingerprinting Hemileia vastatrix |
topic |
Coffee breeding Colletotrichum kahawae Durable resistance Fingerprinting Hemileia vastatrix |
description |
The main phytosanitary problems affecting global coffee production are the fungal diseases known as rust, caused by Hemileia vastatrix Berkeley and Broome, and coffee berry disease (CBD), induced by Colletotrichum kahawae Waller and Bridge. The main disease control strategy is the use of resistant coffee cultivars. Híbrido de Timor is the most important source of resistant varieties used in breeding programs worldwide. The objective of this work was to characterize the diversity and disease resistance of 152 HdT genotypes from the germplasm collection at the Universidade Federal de Viçosa (UFV). Accessions were phenotyped with H. vastatrix races II and XXXIII. Molecular analysis was carried out with 29 random microsatellite markers or single sequence repeats (SSRs), and two SSRs associated with the CBD resistance gene Ck-1. All accessions in the germplasm collection were resistant to H. vastatrix race II, and 141 were resistant to H. vastatrix race XXXIII. Based on the presence of markers, there were 106 accessions containing the CBD resistance gene Ck-1. In the diversity study, the 152 accessions clustered into 21 different groups. A unique molecular profile (fingerprint) was determined for each individual, using 52 alleles from 22 SSR markers. The HdT germplasm of UFV was highly diverse, and included 99 accessions with multiple disease resistance genes, including the CBD resistance gene Ck-1, and others conferring resistance to H. vastatrix races II and XXXIII. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-09-12T17:03:01Z |
dc.date.available.fl_str_mv |
2018-09-12T17:03:01Z |
dc.date.issued.fl_str_mv |
2018-08-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1007/s10681-018-2231-2 http://www.locus.ufv.br/handle/123456789/21783 |
dc.identifier.issn.none.fl_str_mv |
1573-5060 |
identifier_str_mv |
1573-5060 |
url |
https://doi.org/10.1007/s10681-018-2231-2 http://www.locus.ufv.br/handle/123456789/21783 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
Volumes 214, Issues 9, September 2018 |
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Springer Netherlands info:eu-repo/semantics/openAccess |
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Springer Netherlands |
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Euphytica |
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Euphytica |
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