Bactericidal activity of ethanolic extracts of propolis against Staphylococcus aureus isolated from mastitic cows
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1007/s11274-011-0839-7 http://www.locus.ufv.br/handle/123456789/19637 |
Resumo: | Staphylococcus aureus is an important pathogen for both humans and animals, and it has been an ubiquitous etiological agent of bovine mastitis in dairy farms worldwide. Elimination of S. aureus with classic antibiotics is difficult, and the current study aimed to evaluate the efficacy of ethanolic extracts of propolis (EEP) against S. aureus cultivated in complex media or milk. EEP (0–0.5 mg ml^−1) decreased growth of S. aureus in BHI media and 1 mg ml^−1 was bactericidal against washed cell suspensions (10^7 CFU ml−1). Propolis extracts also killed S. aureus cells resuspended in milk, but the bactericidal dose was at least 20-fold greater. Cultures that were transferred for at least 60 generations with sub-lethal doses of propolis did not change much their sensibility to EEP. Atomic force microscopy images revealed changes in morphology and cell size of S. aureus cells exposed to EEP (0.5 mg ml^−1). Our results indicate that propolis extracts might be effective against mastitis-causing S. aureus strains in vivo, but milk constituents affect the inhibitory activity of propolis. Considering that propolis-resistance appears to be a phenotype not easily selected, the use of EEP combined or not with other antimicrobial agents might be useful for mastitis control in vivo. |
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Santana, Henrique FreitasBarbosa, Ana Andréa TeixeiraFerreira, Sukarno OlavoMantovani, Hilário Cuquetto2018-05-17T13:52:23Z2018-05-17T13:52:23Z2011-07-1715730972http://dx.doi.org/10.1007/s11274-011-0839-7http://www.locus.ufv.br/handle/123456789/19637Staphylococcus aureus is an important pathogen for both humans and animals, and it has been an ubiquitous etiological agent of bovine mastitis in dairy farms worldwide. Elimination of S. aureus with classic antibiotics is difficult, and the current study aimed to evaluate the efficacy of ethanolic extracts of propolis (EEP) against S. aureus cultivated in complex media or milk. EEP (0–0.5 mg ml^−1) decreased growth of S. aureus in BHI media and 1 mg ml^−1 was bactericidal against washed cell suspensions (10^7 CFU ml−1). Propolis extracts also killed S. aureus cells resuspended in milk, but the bactericidal dose was at least 20-fold greater. Cultures that were transferred for at least 60 generations with sub-lethal doses of propolis did not change much their sensibility to EEP. Atomic force microscopy images revealed changes in morphology and cell size of S. aureus cells exposed to EEP (0.5 mg ml^−1). Our results indicate that propolis extracts might be effective against mastitis-causing S. aureus strains in vivo, but milk constituents affect the inhibitory activity of propolis. Considering that propolis-resistance appears to be a phenotype not easily selected, the use of EEP combined or not with other antimicrobial agents might be useful for mastitis control in vivo.engWorld Journal of Microbiology and Biotechnologyv. 28, n. 2, p. 485–491, Fevereiro 2012Springer Science+Business Media B.Vinfo:eu-repo/semantics/openAccessBaccharis dracunculifoliaMastitisUdder infectionAFMDairyBactericidal activity of ethanolic extracts of propolis against Staphylococcus aureus isolated from mastitic cowsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf392590https://locus.ufv.br//bitstream/123456789/19637/1/artigo.pdf0c30bcd4715dea21515d3f9dd0ca275cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/19637/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg5088https://locus.ufv.br//bitstream/123456789/19637/3/artigo.pdf.jpg07bb3571d53cee790ab89d15f66379dbMD53123456789/196372018-05-17 23:00:47.106oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-05-18T02:00:47LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Bactericidal activity of ethanolic extracts of propolis against Staphylococcus aureus isolated from mastitic cows |
title |
Bactericidal activity of ethanolic extracts of propolis against Staphylococcus aureus isolated from mastitic cows |
spellingShingle |
Bactericidal activity of ethanolic extracts of propolis against Staphylococcus aureus isolated from mastitic cows Santana, Henrique Freitas Baccharis dracunculifolia Mastitis Udder infection AFM Dairy |
title_short |
Bactericidal activity of ethanolic extracts of propolis against Staphylococcus aureus isolated from mastitic cows |
title_full |
Bactericidal activity of ethanolic extracts of propolis against Staphylococcus aureus isolated from mastitic cows |
title_fullStr |
Bactericidal activity of ethanolic extracts of propolis against Staphylococcus aureus isolated from mastitic cows |
title_full_unstemmed |
Bactericidal activity of ethanolic extracts of propolis against Staphylococcus aureus isolated from mastitic cows |
title_sort |
Bactericidal activity of ethanolic extracts of propolis against Staphylococcus aureus isolated from mastitic cows |
author |
Santana, Henrique Freitas |
author_facet |
Santana, Henrique Freitas Barbosa, Ana Andréa Teixeira Ferreira, Sukarno Olavo Mantovani, Hilário Cuquetto |
author_role |
author |
author2 |
Barbosa, Ana Andréa Teixeira Ferreira, Sukarno Olavo Mantovani, Hilário Cuquetto |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Santana, Henrique Freitas Barbosa, Ana Andréa Teixeira Ferreira, Sukarno Olavo Mantovani, Hilário Cuquetto |
dc.subject.pt-BR.fl_str_mv |
Baccharis dracunculifolia Mastitis Udder infection AFM Dairy |
topic |
Baccharis dracunculifolia Mastitis Udder infection AFM Dairy |
description |
Staphylococcus aureus is an important pathogen for both humans and animals, and it has been an ubiquitous etiological agent of bovine mastitis in dairy farms worldwide. Elimination of S. aureus with classic antibiotics is difficult, and the current study aimed to evaluate the efficacy of ethanolic extracts of propolis (EEP) against S. aureus cultivated in complex media or milk. EEP (0–0.5 mg ml^−1) decreased growth of S. aureus in BHI media and 1 mg ml^−1 was bactericidal against washed cell suspensions (10^7 CFU ml−1). Propolis extracts also killed S. aureus cells resuspended in milk, but the bactericidal dose was at least 20-fold greater. Cultures that were transferred for at least 60 generations with sub-lethal doses of propolis did not change much their sensibility to EEP. Atomic force microscopy images revealed changes in morphology and cell size of S. aureus cells exposed to EEP (0.5 mg ml^−1). Our results indicate that propolis extracts might be effective against mastitis-causing S. aureus strains in vivo, but milk constituents affect the inhibitory activity of propolis. Considering that propolis-resistance appears to be a phenotype not easily selected, the use of EEP combined or not with other antimicrobial agents might be useful for mastitis control in vivo. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-07-17 |
dc.date.accessioned.fl_str_mv |
2018-05-17T13:52:23Z |
dc.date.available.fl_str_mv |
2018-05-17T13:52:23Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11274-011-0839-7 http://www.locus.ufv.br/handle/123456789/19637 |
dc.identifier.issn.none.fl_str_mv |
15730972 |
identifier_str_mv |
15730972 |
url |
http://dx.doi.org/10.1007/s11274-011-0839-7 http://www.locus.ufv.br/handle/123456789/19637 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v. 28, n. 2, p. 485–491, Fevereiro 2012 |
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Springer Science+Business Media B.V info:eu-repo/semantics/openAccess |
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Springer Science+Business Media B.V |
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openAccess |
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World Journal of Microbiology and Biotechnology |
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World Journal of Microbiology and Biotechnology |
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