Espermatogênese no percevejo Podisus Nigrispinus tratado com o bioinseticida Azadiractina

• Autor(a) principal: Santos, Helen Cristina Pinto
• Data de Publicação: 2013
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2367
• topic: Neem; Controle biológico e morfologia testicular; Neem; Biological control and testicular morphology; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

Bedbugs predators, especially of the Pentatomidae family, are natural enemies very used in biological control programs The present study aimed to evaluate if the topical contact of the predator Podisus nigrispinus with azadirachtin at low concentrations causes alterations in testicular morphology and spermatogenesis. Was applied 1 μL de azadirachtin containing 30 ppm (30 Mg / ml) on the scutellum of 20 newly-emerged adults and 30 third instar nymphs after molting. The control treatments were made used distilled water and absolute ethanol. After emergence of nymphs on adult, males were divided into three groups (I, II and III) of 10 insects each. The testes of stinkbugs from group I were dissected 24 houres after emergence, from group II after 7 days after emergence and group III after 15 days after emergence. Out of the 20 adults treated, 10 had their testes dissected with 7 days and another 10 with 15 days. The material was processed for light microscopy and sections of 1 mm in thickness were stained with hematoxylin and toluidine blue. In the P. nigrispinus testis general morphology and spermatogenesis was the same between the treated and control groups. No difference was observed between the treated and control groups. The spermatogonia have spherical shape and a large nucleus with a evident nucleolus adjacent to the nuclear envelope and the spermatocytes have a large nucleus with a granular chromatin homogeneously distributed. Early spermatids have rounded nuclei, smaller than spermatocytes ones, and with peripheral location. Concurrently with the chromatin condensation, the acrosome begins to be formed, and in the opposite pole, the axoneme and mitochondrial complex become longer. After this process the sperm has been formed. Therefore, these results suggest that P. nigrispinus and bioinsecitcida azadirachtin have potential to be used concomitantly in Integrated Pest Management.