Espermatogênese no percevejo Podisus Nigrispinus tratado com o bioinseticida Azadiractina
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/2367 |
Resumo: | Bedbugs predators, especially of the Pentatomidae family, are natural enemies very used in biological control programs The present study aimed to evaluate if the topical contact of the predator Podisus nigrispinus with azadirachtin at low concentrations causes alterations in testicular morphology and spermatogenesis. Was applied 1 μL de azadirachtin containing 30 ppm (30 Mg / ml) on the scutellum of 20 newly-emerged adults and 30 third instar nymphs after molting. The control treatments were made used distilled water and absolute ethanol. After emergence of nymphs on adult, males were divided into three groups (I, II and III) of 10 insects each. The testes of stinkbugs from group I were dissected 24 houres after emergence, from group II after 7 days after emergence and group III after 15 days after emergence. Out of the 20 adults treated, 10 had their testes dissected with 7 days and another 10 with 15 days. The material was processed for light microscopy and sections of 1 mm in thickness were stained with hematoxylin and toluidine blue. In the P. nigrispinus testis general morphology and spermatogenesis was the same between the treated and control groups. No difference was observed between the treated and control groups. The spermatogonia have spherical shape and a large nucleus with a evident nucleolus adjacent to the nuclear envelope and the spermatocytes have a large nucleus with a granular chromatin homogeneously distributed. Early spermatids have rounded nuclei, smaller than spermatocytes ones, and with peripheral location. Concurrently with the chromatin condensation, the acrosome begins to be formed, and in the opposite pole, the axoneme and mitochondrial complex become longer. After this process the sperm has been formed. Therefore, these results suggest that P. nigrispinus and bioinsecitcida azadirachtin have potential to be used concomitantly in Integrated Pest Management. |
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Santos, Helen Cristina Pintohttp://lattes.cnpq.br/6530960479559622Zama, Uyrá dos Santoshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4703213J0Fialho, Maria do Carmo Queirozhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4127872D4Lino Neto, Joséhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786229P1Serrão, José Eduardohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785636U6Lisboa, Luciane Cristina Oliveirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707649T72015-03-26T13:04:19Z2014-06-122015-03-26T13:04:19Z2013-03-18SANTOS, Helen Cristina Pinto. Spermatogenesis of the stinkbug podisus nigrispinus treaty with biopesticide azadirachtin. 2013. 22 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2013.http://locus.ufv.br/handle/123456789/2367Bedbugs predators, especially of the Pentatomidae family, are natural enemies very used in biological control programs The present study aimed to evaluate if the topical contact of the predator Podisus nigrispinus with azadirachtin at low concentrations causes alterations in testicular morphology and spermatogenesis. Was applied 1 μL de azadirachtin containing 30 ppm (30 Mg / ml) on the scutellum of 20 newly-emerged adults and 30 third instar nymphs after molting. The control treatments were made used distilled water and absolute ethanol. After emergence of nymphs on adult, males were divided into three groups (I, II and III) of 10 insects each. The testes of stinkbugs from group I were dissected 24 houres after emergence, from group II after 7 days after emergence and group III after 15 days after emergence. Out of the 20 adults treated, 10 had their testes dissected with 7 days and another 10 with 15 days. The material was processed for light microscopy and sections of 1 mm in thickness were stained with hematoxylin and toluidine blue. In the P. nigrispinus testis general morphology and spermatogenesis was the same between the treated and control groups. No difference was observed between the treated and control groups. The spermatogonia have spherical shape and a large nucleus with a evident nucleolus adjacent to the nuclear envelope and the spermatocytes have a large nucleus with a granular chromatin homogeneously distributed. Early spermatids have rounded nuclei, smaller than spermatocytes ones, and with peripheral location. Concurrently with the chromatin condensation, the acrosome begins to be formed, and in the opposite pole, the axoneme and mitochondrial complex become longer. After this process the sperm has been formed. Therefore, these results suggest that P. nigrispinus and bioinsecitcida azadirachtin have potential to be used concomitantly in Integrated Pest Management.Percevejos predadores, especialmente da família Pentatomidae, são inimigos naturais muito utilizados em programas de controle biológico. O presente estudo buscou avaliar se o contato tópico do predador Podisus nigrispinus com azadiractina em baixas concentrações causa alterações em sua morfologia testicular e espermatogênese. Foi aplicado 1 μL de azadiractina contendo 30 ppm (30 g/mL) sobre o escutelo de 20 adultos recem emergidos e 30 ninfas de terceiro instar logo após a muda. Os tratamentos controle foram feitos usado água destilada e etanol absoluto. Após a emergência das ninfas em adultos, os machos foram divididos em três grupos (I, II e III) de 10 insetos cada. Os testículos dos percevejos do grupo I foram dissecados 24 h após a emergência, do grupo II após 7 dias e do grupo III após 15 dias. Dos 20 adultos tratados, 10 tiveram seus testículos dissecados com 7 dias e outros 10 com 15 dias. O material foi processado para microscopia de luz e cortes de 1 μm de espessura foram corados com hematoxilina e azul de toluidina. Na morfologia geral dos testículos e na espermatogênese de P. nigrispinus não se observou diferença entre os grupos tratados e controle. As espermatogônias apresentam formato esférico e um grande núcleo com um nucléolo evidente e adjacente ao envelope nuclear e os espermatócitos apresentam um grande núcleo com cromatina granular e homogeneamente distribuída. Às espermátides iniciais apresentam núcleo arredondado, com localização periférica e menor que aquele dos espermatócitos. Concomitantemente à condensação da cromatina, o acrossomo começa a ser formado e, no polo oposto, o complexo mitocondrial e o axonema alongam-se. Após esse processo tem-se o espermatozoide formado. Portanto, estes resultados sugerem que P. nigrispinus e o bioinsecitcida azadiractina apresentam potencial para serem usados em concomitância no Manejo Integrado de Pragas.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em Biologia Celular e EstruturalUFVBRAnálises quantitativas e moleculares do Genoma; Biologia das células e dos tecidosNeemControle biológico e morfologia testicularNeemBiological control and testicular morphologyCNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERALEspermatogênese no percevejo Podisus Nigrispinus tratado com o bioinseticida AzadiractinaSpermatogenesis of the stinkbug podisus nigrispinus treaty with biopesticide azadirachtininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf368124https://locus.ufv.br//bitstream/123456789/2367/1/texto%20completo.pdf7f52542815f15d4473edf69cf6ebab6eMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain37560https://locus.ufv.br//bitstream/123456789/2367/2/texto%20completo.pdf.txt15a2683c76b874b33aa9e9855102c6ddMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3561https://locus.ufv.br//bitstream/123456789/2367/3/texto%20completo.pdf.jpg4e55e4f230604f05c8c892aa7edf3d74MD53123456789/23672016-04-08 23:06:28.963oai:locus.ufv.br:123456789/2367Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-09T02:06:28LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Espermatogênese no percevejo Podisus Nigrispinus tratado com o bioinseticida Azadiractina |
dc.title.alternative.eng.fl_str_mv |
Spermatogenesis of the stinkbug podisus nigrispinus treaty with biopesticide azadirachtin |
title |
Espermatogênese no percevejo Podisus Nigrispinus tratado com o bioinseticida Azadiractina |
spellingShingle |
Espermatogênese no percevejo Podisus Nigrispinus tratado com o bioinseticida Azadiractina Santos, Helen Cristina Pinto Neem Controle biológico e morfologia testicular Neem Biological control and testicular morphology CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
title_short |
Espermatogênese no percevejo Podisus Nigrispinus tratado com o bioinseticida Azadiractina |
title_full |
Espermatogênese no percevejo Podisus Nigrispinus tratado com o bioinseticida Azadiractina |
title_fullStr |
Espermatogênese no percevejo Podisus Nigrispinus tratado com o bioinseticida Azadiractina |
title_full_unstemmed |
Espermatogênese no percevejo Podisus Nigrispinus tratado com o bioinseticida Azadiractina |
title_sort |
Espermatogênese no percevejo Podisus Nigrispinus tratado com o bioinseticida Azadiractina |
author |
Santos, Helen Cristina Pinto |
author_facet |
Santos, Helen Cristina Pinto |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/6530960479559622 |
dc.contributor.author.fl_str_mv |
Santos, Helen Cristina Pinto |
dc.contributor.advisor-co1.fl_str_mv |
Zama, Uyrá dos Santos |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4703213J0 |
dc.contributor.advisor-co2.fl_str_mv |
Fialho, Maria do Carmo Queiroz |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4127872D4 |
dc.contributor.advisor1.fl_str_mv |
Lino Neto, José |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786229P1 |
dc.contributor.referee1.fl_str_mv |
Serrão, José Eduardo |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785636U6 |
dc.contributor.referee2.fl_str_mv |
Lisboa, Luciane Cristina Oliveira |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707649T7 |
contributor_str_mv |
Zama, Uyrá dos Santos Fialho, Maria do Carmo Queiroz Lino Neto, José Serrão, José Eduardo Lisboa, Luciane Cristina Oliveira |
dc.subject.por.fl_str_mv |
Neem Controle biológico e morfologia testicular |
topic |
Neem Controle biológico e morfologia testicular Neem Biological control and testicular morphology CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
dc.subject.eng.fl_str_mv |
Neem Biological control and testicular morphology |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
description |
Bedbugs predators, especially of the Pentatomidae family, are natural enemies very used in biological control programs The present study aimed to evaluate if the topical contact of the predator Podisus nigrispinus with azadirachtin at low concentrations causes alterations in testicular morphology and spermatogenesis. Was applied 1 μL de azadirachtin containing 30 ppm (30 Mg / ml) on the scutellum of 20 newly-emerged adults and 30 third instar nymphs after molting. The control treatments were made used distilled water and absolute ethanol. After emergence of nymphs on adult, males were divided into three groups (I, II and III) of 10 insects each. The testes of stinkbugs from group I were dissected 24 houres after emergence, from group II after 7 days after emergence and group III after 15 days after emergence. Out of the 20 adults treated, 10 had their testes dissected with 7 days and another 10 with 15 days. The material was processed for light microscopy and sections of 1 mm in thickness were stained with hematoxylin and toluidine blue. In the P. nigrispinus testis general morphology and spermatogenesis was the same between the treated and control groups. No difference was observed between the treated and control groups. The spermatogonia have spherical shape and a large nucleus with a evident nucleolus adjacent to the nuclear envelope and the spermatocytes have a large nucleus with a granular chromatin homogeneously distributed. Early spermatids have rounded nuclei, smaller than spermatocytes ones, and with peripheral location. Concurrently with the chromatin condensation, the acrosome begins to be formed, and in the opposite pole, the axoneme and mitochondrial complex become longer. After this process the sperm has been formed. Therefore, these results suggest that P. nigrispinus and bioinsecitcida azadirachtin have potential to be used concomitantly in Integrated Pest Management. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-03-18 |
dc.date.available.fl_str_mv |
2014-06-12 2015-03-26T13:04:19Z |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:04:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SANTOS, Helen Cristina Pinto. Spermatogenesis of the stinkbug podisus nigrispinus treaty with biopesticide azadirachtin. 2013. 22 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2013. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/2367 |
identifier_str_mv |
SANTOS, Helen Cristina Pinto. Spermatogenesis of the stinkbug podisus nigrispinus treaty with biopesticide azadirachtin. 2013. 22 f. Dissertação (Mestrado em Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos) - Universidade Federal de Viçosa, Viçosa, 2013. |
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http://locus.ufv.br/handle/123456789/2367 |
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por |
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Universidade Federal de Viçosa |
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Mestrado em Biologia Celular e Estrutural |
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UFV |
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BR |
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Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos |
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Universidade Federal de Viçosa |
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