Análise cariotípica de saguis híbridos do gênero Callithrix no sudeste brasileiro

• Autor(a) principal: Novaes, Camila Moura
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2296
• topic: Saguis; Citogenética; Hibridação; Marmosets; Cytogenetics; Hybridization; CNPQ::CIENCIAS BIOLOGICAS::ZOOLOGIA

The Callithrix genus has six species distributed in the Atlantic Forest biome, from northeastern Brazil to the north of the state of São Paulo, and in regions of Cerrado and Caatinga. Hybrid individuals have been recorded in captive conditions and in areas of natural contact among some species of the genus. Conventional cytogenetic studies were performed with the genus Callithrix, however, there are no works in the literature about molecular cytogenetic technique of FISH (fluorescence in situ hybridization) using repetitive DNA probes. This technique can provide rapid, accurate and economical tool for defining the structure and revealing the organization and evolution of the genome of the species. The aim of this study was to analyze cytogenetically animals (N = 7, four males and three females) from bone marrow preparations, from the IlhaD Água in the state of Rio de Janeiro, considered hybrids of theintroduced species C. jacchus and C. penicillata, using conventional staining, Ag-NOR and C banding techniques and staining with fluorochromes of repetitive DNA probes. The specimens presented 2n = 46 and chromosomal formula 10m+18sm+2st+14t of autosomal chromosomes with NF = 77 for males and NF=78 for females. Although a chromosomal formula and a key number that are not indicated in the literature, the diploid number is consistent with the pattern described for the genus proposed. The form of the Y chromosome was similar to that expected in C.aurita, which is native to the region. The Ag-NOR staining marked the secondary constriction of the short arm of two pairs of chromosomes, similar to the pattern already described for other species except C. jacchus, which also features markings NOR on chromosome Y. The C- banding marks the centromeric region chromosome, confirming thehomogeneity observed in the pure species. The GA(15) in microsatellite regions marked telocentric pairs 16, 17, 20 and 21 chromosomes, and only one member of the chromosome pairs 8 and 15 as suggesting the hybrid nature of these pairs of homologous. The CA(15) microsatellite showed signs in the centromeres of two pairs of telocentric chromosomes and the long arm of a pair of subtelocentric chromosomes, being the sign of one of the homologues, appears stronger. The CAT(10) microsatellite showed stronger signals, preferably in the telomeric regions of the long arms of metacentric and subtelocentric chromosomes. The fourth pair of metacentric chromosomes showed signs in his four arms. The CGG(10) microsatellite showed signs at the centromere of four pairs of telocentric chromosomes, and the GAG(10) showed signals on one pair of telocentric chromosomes. The microsatellites A(30), C(30), GC(15), TA(15) e TAT(10), CAA(10), e CAG(10) did not show any signs chromosome. Marking DAPI showed signs as large blocks in chromosomes and autosomes did not coincide with heterochromatic regions. The karyotypic characters suggest that hybrids have more than two parental species. And this is the first work using chromosomal mapping of repeated sequences of DNA probes in marmosets.