Incidência e progresso de begomovirose do tomateiro sob diferentes sistemas de manejo da mosca-branca em Florestal, MG

Detalhes bibliográficos
Autor(a) principal: Malta, Antônio Wilson de Oliveira
Data de Publicação: 2010
Tipo de documento: Tese
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/1029
Resumo: The genus Begomovirus in the Geminiviridae family includes ssDNA viruses transmitted by the whitefly Bemisia tabaci (Homoptera: Aleyrodidae) to dicot hosts. After the introduction of the B biotype of B. tabaci in Brazil in the early 1990's, begomovirus epidemics became common in tomato crops throughout the country. However, incidence and progress of the disease is highly variable along successive growing seasons in different regions. The objectives of this work were to determine the viral species present in the field and to investigate the spatial and temporal dynamics of the disease under different whitefly management strategies, during 2006 and 2007, in the city of Florestal, Minas Gerais state. Treatments consisted on the combinations of three management strategies (conventional, organic and no management) and two F1 hybrids ('Carmen', susceptible to begomoviruses, and Thaty‟, carrying the Ty-1 gene and which is tolerant to the viruses present in the field in Brazil). The experiment was set up in a randomized block design with 6 replications, in a 3x2 factorial scheme. Ten target plants were mapped in each experimental unit. Whitefly populations were monitored on a weekly basis. A leaf disk was collected from each target plant every two weeks for DNA extraction and begomovirus detection and identification. Visual observations of symptomatic target plants were also registered. Five consecutive plantings were carried out from Jan 2006 until Jan 2008. Only in the fifth and last planting did whitefly populations and begomovirus incidence reach epidemic levels. The following begomoviruses were detected in tomato plants during the fifth planting: Sida micrantha x mosaic virus (SiMMV), Tomato chlorotic mottle virus (ToCMoV), Tomato rugose mosaic virus (ToRMV) and Tomato yellow spot virus (ToYSV). Moreover, Euphorbia yellow mosaic virus (EuMV) was detected in a sample of the ubiquitous weed Euphorbia heterophylla, and ToCMoV in a sample of Sida micrantha. Therefore, four distinct begomoviruses were found in tomatoes. ToCMoV was detected both in tomato and Sida micrantha, and EuMV was detected only in a weed. At the first evaluation (35 days after planting, dap), 73 plants were infected, but only 13 of those were showing symptoms. Disease incidence increased from 35 dap until 80 dap, and then decreased until 95 dap in all treatments, becoming stable until the last evaluation at 110 dap. No statistically significant effects were observed for whitefly management strategy or F1 hybrid, and it was not possible to fit the progress curves obtained into any epidemiological model. Likewise, analysis of the area under the disease progress curve for the different treatments did not indicate any significant effects of either management strategy or F1 hybrid. Based on these results, it is possible to conclude that: (i) the Florestal area has a great species diversity of begomoviruses in tomato; (ii) symptoms in the field are unreliable as indicators of begomovirus incidence and disease progress; (iii) disease progress studies should be carried out along with vector monitoring in the target host in assays with a preestablished spatial distribution; (iv) begomovirus epidemics in tomatoes have the potential to cause significant yield losses when peaking during the flowering stages of the crop, independently of whitefly management practices.
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spelling Malta, Antônio Wilson de Oliveirahttp://lattes.cnpq.br/1460463093917375Mizubuti, Eduardo Seiti Gomidehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785633J8Maffia, Luiz Antôniohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783229P9Zerbini Júnior, Francisco Murilohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783743U5Silva, Derly José Henriques dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723282Z2Lopes, Carlos Albertohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783372D62015-03-26T12:41:54Z2011-09-162015-03-26T12:41:54Z2010-12-27MALTA, Antônio Wilson de Oliveira. Incidence and progress of begomovirus disease in tomatoes under different whitefly management strategies at Florestal, MG. 2010. 112 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2010.http://locus.ufv.br/handle/123456789/1029The genus Begomovirus in the Geminiviridae family includes ssDNA viruses transmitted by the whitefly Bemisia tabaci (Homoptera: Aleyrodidae) to dicot hosts. After the introduction of the B biotype of B. tabaci in Brazil in the early 1990's, begomovirus epidemics became common in tomato crops throughout the country. However, incidence and progress of the disease is highly variable along successive growing seasons in different regions. The objectives of this work were to determine the viral species present in the field and to investigate the spatial and temporal dynamics of the disease under different whitefly management strategies, during 2006 and 2007, in the city of Florestal, Minas Gerais state. Treatments consisted on the combinations of three management strategies (conventional, organic and no management) and two F1 hybrids ('Carmen', susceptible to begomoviruses, and Thaty‟, carrying the Ty-1 gene and which is tolerant to the viruses present in the field in Brazil). The experiment was set up in a randomized block design with 6 replications, in a 3x2 factorial scheme. Ten target plants were mapped in each experimental unit. Whitefly populations were monitored on a weekly basis. A leaf disk was collected from each target plant every two weeks for DNA extraction and begomovirus detection and identification. Visual observations of symptomatic target plants were also registered. Five consecutive plantings were carried out from Jan 2006 until Jan 2008. Only in the fifth and last planting did whitefly populations and begomovirus incidence reach epidemic levels. The following begomoviruses were detected in tomato plants during the fifth planting: Sida micrantha x mosaic virus (SiMMV), Tomato chlorotic mottle virus (ToCMoV), Tomato rugose mosaic virus (ToRMV) and Tomato yellow spot virus (ToYSV). Moreover, Euphorbia yellow mosaic virus (EuMV) was detected in a sample of the ubiquitous weed Euphorbia heterophylla, and ToCMoV in a sample of Sida micrantha. Therefore, four distinct begomoviruses were found in tomatoes. ToCMoV was detected both in tomato and Sida micrantha, and EuMV was detected only in a weed. At the first evaluation (35 days after planting, dap), 73 plants were infected, but only 13 of those were showing symptoms. Disease incidence increased from 35 dap until 80 dap, and then decreased until 95 dap in all treatments, becoming stable until the last evaluation at 110 dap. No statistically significant effects were observed for whitefly management strategy or F1 hybrid, and it was not possible to fit the progress curves obtained into any epidemiological model. Likewise, analysis of the area under the disease progress curve for the different treatments did not indicate any significant effects of either management strategy or F1 hybrid. Based on these results, it is possible to conclude that: (i) the Florestal area has a great species diversity of begomoviruses in tomato; (ii) symptoms in the field are unreliable as indicators of begomovirus incidence and disease progress; (iii) disease progress studies should be carried out along with vector monitoring in the target host in assays with a preestablished spatial distribution; (iv) begomovirus epidemics in tomatoes have the potential to cause significant yield losses when peaking during the flowering stages of the crop, independently of whitefly management practices.O gênero begomovirus da família Geminiviridae inclui espécies virais transmitidas a espécies de plantas dicotiledôneas pela mosca-branca Bemisia tabaci (Homoptera: Aleyrodidae). Após a introdução do biótipo B de B. tabaci no Brasil, no início da década de 1990, surtos de begomoviroses em tomateiro passaram a ser relatados em diversos estados brasileiros. Entretanto, a incidência e o progresso da doença variam de ano para ano nas diferentes regiões. Os objetivos deste trabalho foram investigar as espécies de Begomovirus presentes, a dinâmica da doença causada por begomovirus sob diferentes sistemas de manejo do seu vetor, nos anos de 2006 e 2007, no município de Florestal, estado de Minas Gerais. Os tratamentos consistiram da combinação de três sistemas de manejo (testemunha, convencional e orgânico) em dois híbridos de tomateiro ( Carmen‟, suscetível a geminivirus e Thaty‟ tolerante). O experimento foi montado mapeando-se as parcelas com plantas-alvo (PA‟s) em blocos ao acaso com 6 repetições, em esquema fatorial 3 x 2. Semanalmente procedeu-se o monitoramento da população da mosca-branca para identificar o nível de ação e quinzenalmente coletou-se um disco foliar, em microtubos para extração de DNA total, a fim de detectar e identificar o(s) begomovirus presentes, por meio de hibridização molecular, além de monitorar a incidência da doença e estabelecer a curva de progresso da infecção. Foram registradas durante essas coletas observações visuais de plantas sintomáticas. Cinco plantios foram realizados de janeiro de 2006 até janeiro de 2008. Só no quinto e último ensaio, de agosto de 2007 a janeiro de 2008, ocorreu o maior infestação do inseto vetor e a maior incidência de begomovirose. Os seguintes begomovirus foram detectados nas amostras de tomateiro do ensaio 5: Sida micrantha mosaic virus (SiMMV), Tomato chlorotic mottle virus (ToCMoV), Tomato rugose mosaic virus (ToRMV) e Tomato yellow spot virus (ToYSV). Em duas amostras de plantas invasoras presentes na área experimental identificaram-se Euphorbia yellow mosaic virus (EuMV) em Euphorbia heterophylla, e ToCMoV em Sida micrantha. Portanto, quatro begomovirus ocorreram nos tomateiros. O ToCMoV também ocorreu em Sida micrantha, e o EuMV só ocorreu na invasora. Na primeira avaliação aos 35 dap no ensaio 5 foram detectadas 73 plantas infectadas, porém somente 13 tiveram equivalência com as plantas com sintomas. A incidência de doença foi detectada desde os 35 dap, crescente até os 80 dap em todos os seis tratamentos, caindo aos 95 dap, e, assim mantendo-se até os 110 dap. Não houve efeito de sistemas de manejo, de híbridos e não foi possível ajustar modelos às curvas de progresso obtidas. Da mesma forma, o cálculo das áreas abaixo da curva de progresso da doença, para os diferentes tratamentos, não indicou efeitos significativos dos sistemas de manejo ou dos híbridos. Com base nos resultados conclui-se que: (i) Florestal apresenta grande diversidade de espécies de begomovirus; (ii) sintoma de begomovirose em tomateiro não constitui uma referência confiável para aferir a incidência e dinâmica da doença; (iii) estudos de progresso de begomovirus devem ser conduzidos com monitoramento do vetor no hospedeiro-alvo em ensaios com distribuição espacial pré-estabelecida; (iv) a infecção por begomovirus do tomateiro constitui possibilidade de perdas consideráveis na produção de tomates quando ocorre nas fases de floração e frutificação do tomateiro, aos 65 e 80 dap, em qualquer sistema de manejo de controle do vetor.Fundação de Amparo a Pesquisa do Estado de Minas Geraisapplication/pdfporUniversidade Federal de ViçosaDoutorado em FitopatologiaUFVBREtiologia; Epidemiologia; ControleTomateiroGeminiviridaeBegomovirusMosca-brancaIncidênciaProgressoTomatoesGeminiviridaeBegomovirusWhiteflyIncidenceProgressCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIAIncidência e progresso de begomovirose do tomateiro sob diferentes sistemas de manejo da mosca-branca em Florestal, MGIncidence and progress of begomovirus disease in tomatoes under different whitefly management strategies at Florestal, MGinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf3102238https://locus.ufv.br//bitstream/123456789/1029/1/texto%20completo.pdf2dc0406662e3fa21b399a75cd1a60f02MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain210709https://locus.ufv.br//bitstream/123456789/1029/2/texto%20completo.pdf.txt599f54cc406135a71080f2c7d578809cMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3588https://locus.ufv.br//bitstream/123456789/1029/3/texto%20completo.pdf.jpg4e6f118508eb44d3920d7cd1083cbf6dMD53123456789/10292016-04-06 23:15:37.006oai:locus.ufv.br:123456789/1029Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-07T02:15:37LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Incidência e progresso de begomovirose do tomateiro sob diferentes sistemas de manejo da mosca-branca em Florestal, MG
dc.title.alternative.eng.fl_str_mv Incidence and progress of begomovirus disease in tomatoes under different whitefly management strategies at Florestal, MG
title Incidência e progresso de begomovirose do tomateiro sob diferentes sistemas de manejo da mosca-branca em Florestal, MG
spellingShingle Incidência e progresso de begomovirose do tomateiro sob diferentes sistemas de manejo da mosca-branca em Florestal, MG
Malta, Antônio Wilson de Oliveira
Tomateiro
Geminiviridae
Begomovirus
Mosca-branca
Incidência
Progresso
Tomatoes
Geminiviridae
Begomovirus
Whitefly
Incidence
Progress
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
title_short Incidência e progresso de begomovirose do tomateiro sob diferentes sistemas de manejo da mosca-branca em Florestal, MG
title_full Incidência e progresso de begomovirose do tomateiro sob diferentes sistemas de manejo da mosca-branca em Florestal, MG
title_fullStr Incidência e progresso de begomovirose do tomateiro sob diferentes sistemas de manejo da mosca-branca em Florestal, MG
title_full_unstemmed Incidência e progresso de begomovirose do tomateiro sob diferentes sistemas de manejo da mosca-branca em Florestal, MG
title_sort Incidência e progresso de begomovirose do tomateiro sob diferentes sistemas de manejo da mosca-branca em Florestal, MG
author Malta, Antônio Wilson de Oliveira
author_facet Malta, Antônio Wilson de Oliveira
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/1460463093917375
dc.contributor.author.fl_str_mv Malta, Antônio Wilson de Oliveira
dc.contributor.advisor-co1.fl_str_mv Mizubuti, Eduardo Seiti Gomide
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785633J8
dc.contributor.advisor-co2.fl_str_mv Maffia, Luiz Antônio
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783229P9
dc.contributor.advisor1.fl_str_mv Zerbini Júnior, Francisco Murilo
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783743U5
dc.contributor.referee1.fl_str_mv Silva, Derly José Henriques da
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723282Z2
dc.contributor.referee2.fl_str_mv Lopes, Carlos Alberto
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783372D6
contributor_str_mv Mizubuti, Eduardo Seiti Gomide
Maffia, Luiz Antônio
Zerbini Júnior, Francisco Murilo
Silva, Derly José Henriques da
Lopes, Carlos Alberto
dc.subject.por.fl_str_mv Tomateiro
Geminiviridae
Begomovirus
Mosca-branca
Incidência
Progresso
topic Tomateiro
Geminiviridae
Begomovirus
Mosca-branca
Incidência
Progresso
Tomatoes
Geminiviridae
Begomovirus
Whitefly
Incidence
Progress
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
dc.subject.eng.fl_str_mv Tomatoes
Geminiviridae
Begomovirus
Whitefly
Incidence
Progress
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
description The genus Begomovirus in the Geminiviridae family includes ssDNA viruses transmitted by the whitefly Bemisia tabaci (Homoptera: Aleyrodidae) to dicot hosts. After the introduction of the B biotype of B. tabaci in Brazil in the early 1990's, begomovirus epidemics became common in tomato crops throughout the country. However, incidence and progress of the disease is highly variable along successive growing seasons in different regions. The objectives of this work were to determine the viral species present in the field and to investigate the spatial and temporal dynamics of the disease under different whitefly management strategies, during 2006 and 2007, in the city of Florestal, Minas Gerais state. Treatments consisted on the combinations of three management strategies (conventional, organic and no management) and two F1 hybrids ('Carmen', susceptible to begomoviruses, and Thaty‟, carrying the Ty-1 gene and which is tolerant to the viruses present in the field in Brazil). The experiment was set up in a randomized block design with 6 replications, in a 3x2 factorial scheme. Ten target plants were mapped in each experimental unit. Whitefly populations were monitored on a weekly basis. A leaf disk was collected from each target plant every two weeks for DNA extraction and begomovirus detection and identification. Visual observations of symptomatic target plants were also registered. Five consecutive plantings were carried out from Jan 2006 until Jan 2008. Only in the fifth and last planting did whitefly populations and begomovirus incidence reach epidemic levels. The following begomoviruses were detected in tomato plants during the fifth planting: Sida micrantha x mosaic virus (SiMMV), Tomato chlorotic mottle virus (ToCMoV), Tomato rugose mosaic virus (ToRMV) and Tomato yellow spot virus (ToYSV). Moreover, Euphorbia yellow mosaic virus (EuMV) was detected in a sample of the ubiquitous weed Euphorbia heterophylla, and ToCMoV in a sample of Sida micrantha. Therefore, four distinct begomoviruses were found in tomatoes. ToCMoV was detected both in tomato and Sida micrantha, and EuMV was detected only in a weed. At the first evaluation (35 days after planting, dap), 73 plants were infected, but only 13 of those were showing symptoms. Disease incidence increased from 35 dap until 80 dap, and then decreased until 95 dap in all treatments, becoming stable until the last evaluation at 110 dap. No statistically significant effects were observed for whitefly management strategy or F1 hybrid, and it was not possible to fit the progress curves obtained into any epidemiological model. Likewise, analysis of the area under the disease progress curve for the different treatments did not indicate any significant effects of either management strategy or F1 hybrid. Based on these results, it is possible to conclude that: (i) the Florestal area has a great species diversity of begomoviruses in tomato; (ii) symptoms in the field are unreliable as indicators of begomovirus incidence and disease progress; (iii) disease progress studies should be carried out along with vector monitoring in the target host in assays with a preestablished spatial distribution; (iv) begomovirus epidemics in tomatoes have the potential to cause significant yield losses when peaking during the flowering stages of the crop, independently of whitefly management practices.
publishDate 2010
dc.date.issued.fl_str_mv 2010-12-27
dc.date.available.fl_str_mv 2011-09-16
2015-03-26T12:41:54Z
dc.date.accessioned.fl_str_mv 2015-03-26T12:41:54Z
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dc.identifier.citation.fl_str_mv MALTA, Antônio Wilson de Oliveira. Incidence and progress of begomovirus disease in tomatoes under different whitefly management strategies at Florestal, MG. 2010. 112 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2010.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/1029
identifier_str_mv MALTA, Antônio Wilson de Oliveira. Incidence and progress of begomovirus disease in tomatoes under different whitefly management strategies at Florestal, MG. 2010. 112 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2010.
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