Coexistência e evolução molecular de populações de begomovírus na planta não-cultivada Sida acuta

Detalhes bibliográficos
Autor(a) principal: Godinho, Márcio Tadeu
Data de Publicação: 2014
Tipo de documento: Tese
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/1064
Resumo: The Geminiviridae family includes viruses with genomes consisting of one or two molecules of single-stranded circular DNA, encapsidated by a single structural protein in twinned icosahedral particles. The family includes seven genera defined based on the type of insect vector, host range, genome organization and phylogenetic relationships. Viruses belonging to the genus Begomovirus have one or two genomic components, are transmitted by the whitefly Bemisia tabaci and infect dicotyledonous plants. The occurrence of begomoviruses in cultivated and non-cultivated plants has been reported in Brazil since the 1950s, initially in malvaceous and leguminous hosts, and since the mid-1990s, following the introduction of the B biotype of B. tabaci, also in solanaceous hosts. Several studies have been conducted to characterize the begomoviruses occurring in tomato and bean crops in Brazil, and to evaluate the genetic variability of viral populations in cultivated plants. Studies determining the genetic structure and population dynamics of begomoviruses in non-cultivated hosts are less frequent, although it is accepted that these hosts act as a source of inoculum, and thus may contribute to epidemics in cultivated hosts. This study aimed to characterize and study the genetic variability of begomoviruses infecting the non-cultivated plant Sida acuta, sampled in an area of 10,000 m2. The results showed a high variability among isolates, with the detection of three new begomovirus speceis whose proposed names are Sida acuta mosaic virus (SAMV), Sida golden yellow mosaic virus (SiGYMV) and Sida yellow spot virus (SiYSV). The SAMV population is subdivided into three different strains (S1/S2/S3), with point mutations in the CP and Rep viii genes separating the three strains, and with a possible phenotypic difference between strains S1 and S2. Even in the small sampled area, several plants had mixed infection and pseudorecombination between the different strains. The clones belonging to the species SiGYMV and SiYSV have a genomic organization related to begomoviruses present in the "Old World" (Europe, Asia and Africa), with the presence of an AV2-like ORF present only in begomovirus from that region, and with conserved motifs in the CP and Rep proteins also existing only in the Old World. Thus, the high species diversity and genetic variability of begomoviruses can be sampled even in small areas.
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spelling Godinho, Márcio Tadeuhttp://lattes.cnpq.br/2989231021480966Mizubuti, Eduardo Seiti Gomidehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785633J8Zerbini Júnior, Francisco Murilohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783743U5Cunha, Luis Claudio Vieira dahttp://lattes.cnpq.br/9367947173322278Krause-sakate, Renatehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763879Y2Fontes, Elizabeth Pacheco Batistahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781848H2Carvalho, Claudine Márciahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794965T62015-03-26T12:42:05Z2014-10-032015-03-26T12:42:05Z2014-02-25GODINHO, Márcio Tadeu. Coexistence and molecular evolution of begomovirus populations in the non-cultivated plant Sida acuta. 2014. 82 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2014.http://locus.ufv.br/handle/123456789/1064The Geminiviridae family includes viruses with genomes consisting of one or two molecules of single-stranded circular DNA, encapsidated by a single structural protein in twinned icosahedral particles. The family includes seven genera defined based on the type of insect vector, host range, genome organization and phylogenetic relationships. Viruses belonging to the genus Begomovirus have one or two genomic components, are transmitted by the whitefly Bemisia tabaci and infect dicotyledonous plants. The occurrence of begomoviruses in cultivated and non-cultivated plants has been reported in Brazil since the 1950s, initially in malvaceous and leguminous hosts, and since the mid-1990s, following the introduction of the B biotype of B. tabaci, also in solanaceous hosts. Several studies have been conducted to characterize the begomoviruses occurring in tomato and bean crops in Brazil, and to evaluate the genetic variability of viral populations in cultivated plants. Studies determining the genetic structure and population dynamics of begomoviruses in non-cultivated hosts are less frequent, although it is accepted that these hosts act as a source of inoculum, and thus may contribute to epidemics in cultivated hosts. This study aimed to characterize and study the genetic variability of begomoviruses infecting the non-cultivated plant Sida acuta, sampled in an area of 10,000 m2. The results showed a high variability among isolates, with the detection of three new begomovirus speceis whose proposed names are Sida acuta mosaic virus (SAMV), Sida golden yellow mosaic virus (SiGYMV) and Sida yellow spot virus (SiYSV). The SAMV population is subdivided into three different strains (S1/S2/S3), with point mutations in the CP and Rep viii genes separating the three strains, and with a possible phenotypic difference between strains S1 and S2. Even in the small sampled area, several plants had mixed infection and pseudorecombination between the different strains. The clones belonging to the species SiGYMV and SiYSV have a genomic organization related to begomoviruses present in the "Old World" (Europe, Asia and Africa), with the presence of an AV2-like ORF present only in begomovirus from that region, and with conserved motifs in the CP and Rep proteins also existing only in the Old World. Thus, the high species diversity and genetic variability of begomoviruses can be sampled even in small areas.A família Geminiviridae inclui vírus cujos genomas são compostos por uma ou duas moléculas de DNA fita simples circular, encapsidadas por uma única proteína estrutural em partículas icosaédricas geminadas. A família é composta por sete gêneros definidos com base no tipo de inseto vetor, gama de hospedeiros, relacionamento filogenético e organização genômica. Os vírus pertencentes ao gênero Begomovirus possuem um ou dois componentes genômicos, são transmitidos pela mosca-branca Bemisia tabaci e infectam plantas dicotiledôneas. A incidência de begomovírus em plantas cultivadas e não- cultivadas vem sendo relatada no Brasil desde a década de 1950, inicialmente em malváceas e leguminosas, e a partir da década de 1990, após a introdução do biótipo B de B. tabaci, também em solanáceas. Diversos estudos já foram realizados para caracterizar os begomovírus que ocorrem em tomateiro e feijoeiro no Brasil, e para avaliar a variabilidade genética das populações virais em plantas cultivadas. Estudos determinando a estrutura genética e dinâmica de populações de begomovírus em hospedeiros não-cultivados são menos frequentes, embora seja aceito que estes hospedeiros atuem como fonte de inóculo, podendo portanto contribuir para epidemias em hospedeiros cultivados. Este trabalho teve como objetivos caracterizar e estudar a variabilidade genética de populações de begomovírus infectando a planta não-cultivada Sida acuta em uma área de 10.000 m2. Os resultados indicam uma alta variabilidade entre os isolados, com três novas espécies de begomovírus cujos nomes propostos são Sida acuta mosaic virus (SAMV), Sida golden yellow mosaic virus (SiGYMV) e Sida yellow spot virus (SiYSV). A população de SAMV vi está subdividida em três estirpes (S1/S2/S3), com mutações pontuais nos genes CP e Rep separando as três estirpes, inclusive com uma possível diferença fenotípica entre as estirpes S1 e S2. Mesmo na pequena área amostrada foram encontradas plantas com infecção mista e pseudo-recombinação entre estirpes. Os clones pertencentes às espécies SiGYMV e SiYSV apresentam uma organização genômica relacionada a begomovírus presentes no "Velho Mundo" (Europa, Ásia e África) com a presença de uma ORF tipo AV2 existente apenas em begomovirus daquela região, e de motivos conservados nas sequências dos genes CP e Rep também existentes apenas nos begomovírus do Velho Mundo. Conclui-se que a grande diversidade de espécies e variabilidade genética dos begomovírus pode ser amostrada mesmo em pequenas áreas.application/pdfporUniversidade Federal de ViçosaDoutorado em FitopatologiaUFVBREtiologia; Epidemiologia; ControleBegomovirusEvoluçãoGeminiviridaeNova espécieBegomovirusEvolutionGeminiviridaeNew speciesCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIACoexistência e evolução molecular de populações de begomovírus na planta não-cultivada Sida acutaCoexistence and molecular evolution of begomovirus populations in the non-cultivated plant Sida acutainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf1571876https://locus.ufv.br//bitstream/123456789/1064/1/texto%20completo.pdfd5b3e9b7204d508be1af95563dcd7b8eMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain120055https://locus.ufv.br//bitstream/123456789/1064/2/texto%20completo.pdf.txtddc9c0504811ebb03b5726de8ca40ea1MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3614https://locus.ufv.br//bitstream/123456789/1064/3/texto%20completo.pdf.jpg94b89f8c9c71afd75cdcb6fc642ea5bdMD53123456789/10642016-04-06 23:16:26.261oai:locus.ufv.br:123456789/1064Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-07T02:16:26LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Coexistência e evolução molecular de populações de begomovírus na planta não-cultivada Sida acuta
dc.title.alternative.eng.fl_str_mv Coexistence and molecular evolution of begomovirus populations in the non-cultivated plant Sida acuta
title Coexistência e evolução molecular de populações de begomovírus na planta não-cultivada Sida acuta
spellingShingle Coexistência e evolução molecular de populações de begomovírus na planta não-cultivada Sida acuta
Godinho, Márcio Tadeu
Begomovirus
Evolução
Geminiviridae
Nova espécie
Begomovirus
Evolution
Geminiviridae
New species
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
title_short Coexistência e evolução molecular de populações de begomovírus na planta não-cultivada Sida acuta
title_full Coexistência e evolução molecular de populações de begomovírus na planta não-cultivada Sida acuta
title_fullStr Coexistência e evolução molecular de populações de begomovírus na planta não-cultivada Sida acuta
title_full_unstemmed Coexistência e evolução molecular de populações de begomovírus na planta não-cultivada Sida acuta
title_sort Coexistência e evolução molecular de populações de begomovírus na planta não-cultivada Sida acuta
author Godinho, Márcio Tadeu
author_facet Godinho, Márcio Tadeu
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/2989231021480966
dc.contributor.author.fl_str_mv Godinho, Márcio Tadeu
dc.contributor.advisor-co1.fl_str_mv Mizubuti, Eduardo Seiti Gomide
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785633J8
dc.contributor.advisor1.fl_str_mv Zerbini Júnior, Francisco Murilo
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783743U5
dc.contributor.referee1.fl_str_mv Cunha, Luis Claudio Vieira da
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/9367947173322278
dc.contributor.referee2.fl_str_mv Krause-sakate, Renate
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763879Y2
dc.contributor.referee3.fl_str_mv Fontes, Elizabeth Pacheco Batista
dc.contributor.referee3Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781848H2
dc.contributor.referee4.fl_str_mv Carvalho, Claudine Márcia
dc.contributor.referee4Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794965T6
contributor_str_mv Mizubuti, Eduardo Seiti Gomide
Zerbini Júnior, Francisco Murilo
Cunha, Luis Claudio Vieira da
Krause-sakate, Renate
Fontes, Elizabeth Pacheco Batista
Carvalho, Claudine Márcia
dc.subject.por.fl_str_mv Begomovirus
Evolução
Geminiviridae
Nova espécie
topic Begomovirus
Evolução
Geminiviridae
Nova espécie
Begomovirus
Evolution
Geminiviridae
New species
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
dc.subject.eng.fl_str_mv Begomovirus
Evolution
Geminiviridae
New species
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
description The Geminiviridae family includes viruses with genomes consisting of one or two molecules of single-stranded circular DNA, encapsidated by a single structural protein in twinned icosahedral particles. The family includes seven genera defined based on the type of insect vector, host range, genome organization and phylogenetic relationships. Viruses belonging to the genus Begomovirus have one or two genomic components, are transmitted by the whitefly Bemisia tabaci and infect dicotyledonous plants. The occurrence of begomoviruses in cultivated and non-cultivated plants has been reported in Brazil since the 1950s, initially in malvaceous and leguminous hosts, and since the mid-1990s, following the introduction of the B biotype of B. tabaci, also in solanaceous hosts. Several studies have been conducted to characterize the begomoviruses occurring in tomato and bean crops in Brazil, and to evaluate the genetic variability of viral populations in cultivated plants. Studies determining the genetic structure and population dynamics of begomoviruses in non-cultivated hosts are less frequent, although it is accepted that these hosts act as a source of inoculum, and thus may contribute to epidemics in cultivated hosts. This study aimed to characterize and study the genetic variability of begomoviruses infecting the non-cultivated plant Sida acuta, sampled in an area of 10,000 m2. The results showed a high variability among isolates, with the detection of three new begomovirus speceis whose proposed names are Sida acuta mosaic virus (SAMV), Sida golden yellow mosaic virus (SiGYMV) and Sida yellow spot virus (SiYSV). The SAMV population is subdivided into three different strains (S1/S2/S3), with point mutations in the CP and Rep viii genes separating the three strains, and with a possible phenotypic difference between strains S1 and S2. Even in the small sampled area, several plants had mixed infection and pseudorecombination between the different strains. The clones belonging to the species SiGYMV and SiYSV have a genomic organization related to begomoviruses present in the "Old World" (Europe, Asia and Africa), with the presence of an AV2-like ORF present only in begomovirus from that region, and with conserved motifs in the CP and Rep proteins also existing only in the Old World. Thus, the high species diversity and genetic variability of begomoviruses can be sampled even in small areas.
publishDate 2014
dc.date.available.fl_str_mv 2014-10-03
2015-03-26T12:42:05Z
dc.date.issued.fl_str_mv 2014-02-25
dc.date.accessioned.fl_str_mv 2015-03-26T12:42:05Z
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dc.identifier.citation.fl_str_mv GODINHO, Márcio Tadeu. Coexistence and molecular evolution of begomovirus populations in the non-cultivated plant Sida acuta. 2014. 82 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2014.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/1064
identifier_str_mv GODINHO, Márcio Tadeu. Coexistence and molecular evolution of begomovirus populations in the non-cultivated plant Sida acuta. 2014. 82 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2014.
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