Hydrophobic interaction adsorption of hen egg white proteins albumin, conalbumin, and lysozyme
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1016/j.jchromb.2006.04.033 http://www.locus.ufv.br/handle/123456789/19461 |
Resumo: | Hydrophobic adsorption equilibrium data of the hen egg white proteins albumin, conalbumin, and lysozyme were obtained in batch systems, at 25 °C, using the Streamline Phenyl® resin as adsorbent. The influence of three types of salt, NaCl, Na2SO4, or (NH4)2SO4, and their concentration on the equilibrium data were evaluated. The salt Na2SO4 showed the higher interaction with the studied proteins, thus favoring the adsorption of proteins by the adsorbent, even though each type of salt interacted in a distinct manner with each protein. The isotherm models of Langmuir, Langmuir exponential, and Chen and Sun were well fitted to the equilibrium data, with no significant difference being observed at the 5% level of significance. The mass transfer model applied simulated correctly adsorption kinetics of the proteins under the studied conditions. |
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Rojas, Edwin E. GarciaCoimbra, Jane S. dos ReisMinim, Luis A.Saraiva, Sérgio H.Silva, César A. Sodré da2018-05-10T16:36:25Z2018-05-10T16:36:25Z2006-08-1815700232https://doi.org/10.1016/j.jchromb.2006.04.033http://www.locus.ufv.br/handle/123456789/19461Hydrophobic adsorption equilibrium data of the hen egg white proteins albumin, conalbumin, and lysozyme were obtained in batch systems, at 25 °C, using the Streamline Phenyl® resin as adsorbent. The influence of three types of salt, NaCl, Na2SO4, or (NH4)2SO4, and their concentration on the equilibrium data were evaluated. The salt Na2SO4 showed the higher interaction with the studied proteins, thus favoring the adsorption of proteins by the adsorbent, even though each type of salt interacted in a distinct manner with each protein. The isotherm models of Langmuir, Langmuir exponential, and Chen and Sun were well fitted to the equilibrium data, with no significant difference being observed at the 5% level of significance. The mass transfer model applied simulated correctly adsorption kinetics of the proteins under the studied conditions.engJournal of Chromatography Bv. 840, n. 2, p. 85-93, aug. 2006Elsevier B.V.info:eu-repo/semantics/openAccessHydrophobic adsorptionEgg white proteinsModelingHydrophobic interaction adsorption of hen egg white proteins albumin, conalbumin, and lysozymeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf422841https://locus.ufv.br//bitstream/123456789/19461/1/artigo.pdffeea2a3b4b5a99709b95248963804cd6MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/19461/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg5189https://locus.ufv.br//bitstream/123456789/19461/3/artigo.pdf.jpg0ce1445b5a0ce4c8833bff8cf49e5f8bMD53123456789/194612018-05-10 23:00:38.927oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-05-11T02:00:38LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Hydrophobic interaction adsorption of hen egg white proteins albumin, conalbumin, and lysozyme |
title |
Hydrophobic interaction adsorption of hen egg white proteins albumin, conalbumin, and lysozyme |
spellingShingle |
Hydrophobic interaction adsorption of hen egg white proteins albumin, conalbumin, and lysozyme Rojas, Edwin E. Garcia Hydrophobic adsorption Egg white proteins Modeling |
title_short |
Hydrophobic interaction adsorption of hen egg white proteins albumin, conalbumin, and lysozyme |
title_full |
Hydrophobic interaction adsorption of hen egg white proteins albumin, conalbumin, and lysozyme |
title_fullStr |
Hydrophobic interaction adsorption of hen egg white proteins albumin, conalbumin, and lysozyme |
title_full_unstemmed |
Hydrophobic interaction adsorption of hen egg white proteins albumin, conalbumin, and lysozyme |
title_sort |
Hydrophobic interaction adsorption of hen egg white proteins albumin, conalbumin, and lysozyme |
author |
Rojas, Edwin E. Garcia |
author_facet |
Rojas, Edwin E. Garcia Coimbra, Jane S. dos Reis Minim, Luis A. Saraiva, Sérgio H. Silva, César A. Sodré da |
author_role |
author |
author2 |
Coimbra, Jane S. dos Reis Minim, Luis A. Saraiva, Sérgio H. Silva, César A. Sodré da |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Rojas, Edwin E. Garcia Coimbra, Jane S. dos Reis Minim, Luis A. Saraiva, Sérgio H. Silva, César A. Sodré da |
dc.subject.pt-BR.fl_str_mv |
Hydrophobic adsorption Egg white proteins Modeling |
topic |
Hydrophobic adsorption Egg white proteins Modeling |
description |
Hydrophobic adsorption equilibrium data of the hen egg white proteins albumin, conalbumin, and lysozyme were obtained in batch systems, at 25 °C, using the Streamline Phenyl® resin as adsorbent. The influence of three types of salt, NaCl, Na2SO4, or (NH4)2SO4, and their concentration on the equilibrium data were evaluated. The salt Na2SO4 showed the higher interaction with the studied proteins, thus favoring the adsorption of proteins by the adsorbent, even though each type of salt interacted in a distinct manner with each protein. The isotherm models of Langmuir, Langmuir exponential, and Chen and Sun were well fitted to the equilibrium data, with no significant difference being observed at the 5% level of significance. The mass transfer model applied simulated correctly adsorption kinetics of the proteins under the studied conditions. |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006-08-18 |
dc.date.accessioned.fl_str_mv |
2018-05-10T16:36:25Z |
dc.date.available.fl_str_mv |
2018-05-10T16:36:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1016/j.jchromb.2006.04.033 http://www.locus.ufv.br/handle/123456789/19461 |
dc.identifier.issn.none.fl_str_mv |
15700232 |
identifier_str_mv |
15700232 |
url |
https://doi.org/10.1016/j.jchromb.2006.04.033 http://www.locus.ufv.br/handle/123456789/19461 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v. 840, n. 2, p. 85-93, aug. 2006 |
dc.rights.driver.fl_str_mv |
Elsevier B.V. info:eu-repo/semantics/openAccess |
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Elsevier B.V. |
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openAccess |
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dc.publisher.none.fl_str_mv |
Journal of Chromatography B |
publisher.none.fl_str_mv |
Journal of Chromatography B |
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