Hydrophobic interaction adsorption of hen egg white proteins albumin, conalbumin, and lysozyme

Detalhes bibliográficos
Autor(a) principal: Rojas, Edwin E. Garcia
Data de Publicação: 2006
Outros Autores: Coimbra, Jane S. dos Reis, Minim, Luis A., Saraiva, Sérgio H., Silva, César A. Sodré da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1016/j.jchromb.2006.04.033
http://www.locus.ufv.br/handle/123456789/19461
Resumo: Hydrophobic adsorption equilibrium data of the hen egg white proteins albumin, conalbumin, and lysozyme were obtained in batch systems, at 25 °C, using the Streamline Phenyl® resin as adsorbent. The influence of three types of salt, NaCl, Na2SO4, or (NH4)2SO4, and their concentration on the equilibrium data were evaluated. The salt Na2SO4 showed the higher interaction with the studied proteins, thus favoring the adsorption of proteins by the adsorbent, even though each type of salt interacted in a distinct manner with each protein. The isotherm models of Langmuir, Langmuir exponential, and Chen and Sun were well fitted to the equilibrium data, with no significant difference being observed at the 5% level of significance. The mass transfer model applied simulated correctly adsorption kinetics of the proteins under the studied conditions.
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spelling Rojas, Edwin E. GarciaCoimbra, Jane S. dos ReisMinim, Luis A.Saraiva, Sérgio H.Silva, César A. Sodré da2018-05-10T16:36:25Z2018-05-10T16:36:25Z2006-08-1815700232https://doi.org/10.1016/j.jchromb.2006.04.033http://www.locus.ufv.br/handle/123456789/19461Hydrophobic adsorption equilibrium data of the hen egg white proteins albumin, conalbumin, and lysozyme were obtained in batch systems, at 25 °C, using the Streamline Phenyl® resin as adsorbent. The influence of three types of salt, NaCl, Na2SO4, or (NH4)2SO4, and their concentration on the equilibrium data were evaluated. The salt Na2SO4 showed the higher interaction with the studied proteins, thus favoring the adsorption of proteins by the adsorbent, even though each type of salt interacted in a distinct manner with each protein. The isotherm models of Langmuir, Langmuir exponential, and Chen and Sun were well fitted to the equilibrium data, with no significant difference being observed at the 5% level of significance. The mass transfer model applied simulated correctly adsorption kinetics of the proteins under the studied conditions.engJournal of Chromatography Bv. 840, n. 2, p. 85-93, aug. 2006Elsevier B.V.info:eu-repo/semantics/openAccessHydrophobic adsorptionEgg white proteinsModelingHydrophobic interaction adsorption of hen egg white proteins albumin, conalbumin, and lysozymeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf422841https://locus.ufv.br//bitstream/123456789/19461/1/artigo.pdffeea2a3b4b5a99709b95248963804cd6MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/19461/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg5189https://locus.ufv.br//bitstream/123456789/19461/3/artigo.pdf.jpg0ce1445b5a0ce4c8833bff8cf49e5f8bMD53123456789/194612018-05-10 23:00:38.927oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-05-11T02:00:38LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Hydrophobic interaction adsorption of hen egg white proteins albumin, conalbumin, and lysozyme
title Hydrophobic interaction adsorption of hen egg white proteins albumin, conalbumin, and lysozyme
spellingShingle Hydrophobic interaction adsorption of hen egg white proteins albumin, conalbumin, and lysozyme
Rojas, Edwin E. Garcia
Hydrophobic adsorption
Egg white proteins
Modeling
title_short Hydrophobic interaction adsorption of hen egg white proteins albumin, conalbumin, and lysozyme
title_full Hydrophobic interaction adsorption of hen egg white proteins albumin, conalbumin, and lysozyme
title_fullStr Hydrophobic interaction adsorption of hen egg white proteins albumin, conalbumin, and lysozyme
title_full_unstemmed Hydrophobic interaction adsorption of hen egg white proteins albumin, conalbumin, and lysozyme
title_sort Hydrophobic interaction adsorption of hen egg white proteins albumin, conalbumin, and lysozyme
author Rojas, Edwin E. Garcia
author_facet Rojas, Edwin E. Garcia
Coimbra, Jane S. dos Reis
Minim, Luis A.
Saraiva, Sérgio H.
Silva, César A. Sodré da
author_role author
author2 Coimbra, Jane S. dos Reis
Minim, Luis A.
Saraiva, Sérgio H.
Silva, César A. Sodré da
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rojas, Edwin E. Garcia
Coimbra, Jane S. dos Reis
Minim, Luis A.
Saraiva, Sérgio H.
Silva, César A. Sodré da
dc.subject.pt-BR.fl_str_mv Hydrophobic adsorption
Egg white proteins
Modeling
topic Hydrophobic adsorption
Egg white proteins
Modeling
description Hydrophobic adsorption equilibrium data of the hen egg white proteins albumin, conalbumin, and lysozyme were obtained in batch systems, at 25 °C, using the Streamline Phenyl® resin as adsorbent. The influence of three types of salt, NaCl, Na2SO4, or (NH4)2SO4, and their concentration on the equilibrium data were evaluated. The salt Na2SO4 showed the higher interaction with the studied proteins, thus favoring the adsorption of proteins by the adsorbent, even though each type of salt interacted in a distinct manner with each protein. The isotherm models of Langmuir, Langmuir exponential, and Chen and Sun were well fitted to the equilibrium data, with no significant difference being observed at the 5% level of significance. The mass transfer model applied simulated correctly adsorption kinetics of the proteins under the studied conditions.
publishDate 2006
dc.date.issued.fl_str_mv 2006-08-18
dc.date.accessioned.fl_str_mv 2018-05-10T16:36:25Z
dc.date.available.fl_str_mv 2018-05-10T16:36:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv https://doi.org/10.1016/j.jchromb.2006.04.033
http://www.locus.ufv.br/handle/123456789/19461
dc.identifier.issn.none.fl_str_mv 15700232
identifier_str_mv 15700232
url https://doi.org/10.1016/j.jchromb.2006.04.033
http://www.locus.ufv.br/handle/123456789/19461
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv v. 840, n. 2, p. 85-93, aug. 2006
dc.rights.driver.fl_str_mv Elsevier B.V.
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publisher.none.fl_str_mv Journal of Chromatography B
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