Avaliação da ingestão aguda e crônica da polpa de manga Ubá (Mangifera indica L.) na homeostase sérica, na expressão da HSP 70 e na atividade da superóxido dismutase em ratos

• Autor(a) principal: Toledo, Renata Celi Lopes
• Data de Publicação: 2010
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2424
• topic: Manga Ubá; Homeostase sérica; HSP70; Ubá mango; Serum homeostasis; HSP70; CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::METABOLISMO E BIOENERGETICA

The mango (Mangifera indica L.) is among the tropical fruits of greater economic impact in the Brazilian and international markets. The Ubá variety is very productive and well known in some regions of Brazil, especially in the Zona da Mata de Minas Gerais. This fruit is known for its nutritional value, being a rich source of ascorbic acid, carotenoids and phenolic compounds, molecules that have great potential in biological antioxidant. Epidemiological studies have shown evidence that antioxidants present in fruits and vegetables are the main factors contributing to the low incidence of diseases related to oxidative stress. This study aimed to evaluate the effect of acute and chronic ingestion of diets supplemented with 3% and 10% mango pulp Ubá in the modulation of biomarkers of endogenous antioxidant system (superoxide dismutase and HSP 70) and biochemical markers of oxidative stress (aminotransferase enzymes and malondialdehyde) in healthy Wistar rats. The results showed that intake of diets supplemented with 3% and 10% mango pulp showed affect on markers of injuries once that in the experiment of 24 hours the animals fed diets with 3% mango pulp diets showed level of serum AST lower that control and level serum GGT lesser than the animals fed diets with 10% mango pulp. Furthermore, in the experiment of 25 days, the animals fed diets with 10% of mango had higher serum ALT that animals fed diets supplemented with vitamin E. Analysis of lipid peroxidation marker in the 25 day experiment showed that animals fed diets with 10% mango pulp Ubá showed higher malondialdehyde concentrations than those fed control diet and 3% mango. Animals receiving the diet supplemented with 3% mango pulp in the experiment of 24 hours showed decreased activity of superoxide dismutase as compared to control, a result that was not found in chronic ingestion of this diet. The HSP 70 gene expression did not change with the diets supplemented with vitamin E and mango pulp, but the animals fed diets with 10% of mango in the experiment of 25 days showed a significant reduction in the expression of HSP70 genes compared with animals received this diet in the experiment of 24 hours. Therefore, the present results indicate that the antioxidant present in the diet supplemented with 3 and 10% mango pulp Ubá ingested acutely and chronically may interfere with antioxidant defense mechanisms and markers of liver injury, since supplementation with 3 % mango pulp Ubá ingested per 24 hours decreased the concentration of AST and hepatic SOD activity, whereas the intake per 25 days of 10% mango pulp increased lipid peroxidation in the liver of healthy Wistar rats.