Efeito do extrato das folhas de manga Ubá (Mangifera indica L) e da mangiferina na modulação da expressão dos receptores CB1, PPARγ e adipocinas em ratos alimentados com dieta de cafeteria

• Autor(a) principal: Brito, Larissa Froede
• Data de Publicação: 2013
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/332
• topic: Manga Ubá; Extrato; Receptores CB1; PPARy; Mango Ubá; Extract; CB1 receptors; PPAR; CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::METABOLISMO E BIOENERGETICA

Obesity is a multifactorial disease strongly associated with adipogenesis, inflammation and metabolic syndrome. The distribution of body fat is an important factor, and specifically adipose tissue, visceral and subcutaneous seem to be the link between weight gain, adipogenesis, inflammation and metabolic risk. Adipocytes are involved in the regulation of energy balance and metabolism can be modulated by hormones, cytokines and nutrients, including adiponectin, leptin and resistin. And PPAR gamma CB1 receptors are involved in regulating the production of adipocytes, and insulin sensitivity in the inflammatory process. Activation of PPAR gamma triggers anti- inflammatory activities in several cell types by modulating the expression of pro- inflammatory genes. As for the CB1 receptors are directly related to obesity and other metabolic disorders, and, in certain cases behave as other pro-inflammatory and as anti- inflammatory agents. The mango leaf extract and mangiferin (main bioactive compound isolated from the extract), possess pharmacological antioxidant, anti-inflammatory, antiadipogênica, among others. The aim of the study was to evaluate the effect of leaf extract of mango and mangiferin isolated the same, prophylaxis and treatment in adipogenesis, through weight gain, biochemical and gene expression of CB1 receptors, PPAR gamma and the adipokines adiponectin, resistin and leptin in adipose tissue of rats fed a cafeteria diet. The mango leaf extract and mangiferin were administered to the animals by gavage at a dose of 40mg/kg and 250mg/kg respectively. Before the start of the experiments the animals were adapted for 8 days. The study was divided into two stages: prevention and treatment. Prophylaxis animals receberm the cafeteria diet, the leaf extract and mangiferin Mango together for eight days. In the treatment period, the animals received only cafeteria diet for 8 days to stimulate adipogenesis and then treated with the leaf extract of the sleeve, the mangiferin and CB1 receptor antagonists and PPAR gamma for 5 days. For weight gain, weight gain and food consumption were monitored daily. After each trial, the animals were euthanized, where blood was withdrawn to obtain the serum for performing the biochemical analysis of triacylglycerols, glucose, total cholesterol and its fractions (performed by colorimetric xkits) and adipose tissue (visceral and subcutaneous ) removed for analysis of gene expression of adipokines receivers and proposals (obtained by RT-PCR). At the trial concerning the prophylaxis, a significant decrease in food intake in the group receiving the mango leaf extract and weight of adipose tissue was significantly lower in the groups treated with the extract and mangiferin, compared to the control group. In the group of animals receiving extracts a significant reduction in the expression of CB1 receptor and resistin, adiponectin as presented a statistically higher expression compared to the control. In the group of animals receiving mangiferin, there was a significant increase in the expression of CB1 and a statistically significant decrease in expression of adipokines leptin and adiponectin. In the trial for the treatment groups treated with leaf extract and mangiferin Mango, significantly decreased the accumulation of adipose tissue and triglyceride levels while HDL levels increased significantly compared to the control group. In the group treated with the extract and the expression of the leptin receptor CB1 was statistically significantly higher, while adiponectin were significantly lower. The data showed a possible anti-inflammatory effect of mangiferin and antiadipogênico, since the expression of CB1 and PPAR gamma and adipokines resistin and leptin were significantly higher and that the expression of adiponectin decreased significantly compared to the control group. In this sense, the study showed the beneficial effect of the leaf extract of mango and mangiferin in the prophylaxis and treatment of adipogenesis, inflammation and metabolic risk factors associated with obesity and metabolic syndrome.