Germinação de sementes de Melanoxylon brauna (SCHOTT) sob diferentes temperaturas: aspectos morfofisiológicos e enzimáticos

Detalhes bibliográficos
Autor(a) principal: Flores, Andressa Vasconcelos
Data de Publicação: 2011
Tipo de documento: Tese
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/558
Resumo: The germination of tropical tree species needs more precise information on the physical and intrinsic factors that may interfere in the germination process. Given this fact, this study aimed to investigate the morphometric characteristics and increasing levels of temperature and its implications on the physiology and metabolism of Melanoxylon brauna Schott seeds during the germination process. To this end, the experiments were conducted at the Laboratory of Seed Analysis of Forestry in Department of Forestry, University Federal of Viçosa - UFV. The seeds used in this study were collected from 10 trees in the city of Leopoldina - MG. In the first chapter, two assays were conducted, the first to evaluate the effect of color and size, in which the seeds were visually classified into light and dark, and in relation to size in large and small, and then were sown in two pieces of filter paper. In the second assay, were evaluated the effect of different substrates on germination. Thus, were tested the substrates on sand (SA), between sand (EA), on paper germitest (SP), between paper germitest (EP) and paper roll germitest (RP). In both trials, seeds were placed in BOD under constant temperature of 25°C and constant light for 10 days, and evaluated the characteristics of germination speed index (GSI) and percentage of germination. In the second chapter were carried out three assays. In the first were determined curves of soaking at temperatures of 5, 10, 15, 20, 25, 30, 35, 40 and 45°C. In the second trial, to identify the cardinal temperatures, seeds were kept under each temperature for a period of 10 days in BOD, under constant light. For the third test, was carried out electrical conductivity test (EC) with the seeds incubated for 24, 48 and 72 hours of immersion in each of the temperatures mentioned above, and then they were placed in erlenmeyers with 75 mL deionized water at 25°C, for 24 hours for subsequent reading of conductivity. In the third chapter, enzymatic analysis was performed. Measurements of the activities of superoxide dismutase (SOD, EC 1.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11) and catalase (CAT, EC 1.11.1.6) were made in the micropylar endosperm and embryonic axis of seeds germinated under the same conditions described above, at temperatures of 15, 25, 30 and 40°C, and every 12 hours until the samples were taken root protrusion of 50% of the seeds or 168 hours to a temperature of 15°C. Seeds small and light have lower germination e IVG that the small and large, regardless of color. The substrate most suitable for the testing laboratory germination is the paper roll germitest. The temperature appeared significantly in germination and synchronization of Melanoxylon brauna seeds. The cardinal temperatures for germination (%) are: 12.3°C - minimum 30.0°C - optimal (theoretical) and 42.7°C maximum. The temperature ranges considering all the variables investigated were: 12.1 to 12.6°C (minimum); 30 to 35.8 ° C (optimum) and 42.4 to 43°C (maximum). The specie has a wide range of germination with respect to temperature, germination was observed in the temperature range from 12 to 42°C. The EC test was efficient to evaluate the effect of temperature on the physiology and according to this, the optimum temperature is 27°C. Did not detect the activity of SOD, APX and CAT in the micropylar endosperm during seed germination under different temperatures. In the embryonic axis, the activities of SOD and CAT showed greater changes along the germination when subjected to temperatures of 15 and 40°C. The APX enzyme activity in the embryonic axis was not affected by temperature during germination and its activity was extremely low.
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spelling Flores, Andressa Vasconceloshttp://lattes.cnpq.br/1039066389051551Guimarães, Valéria Montezehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798758T3Gonçalves, José Francisco de Carvalhohttp://lattes.cnpq.br/0553096006639259Borges, Eduardo Euclydes de Lima ehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787799U8Araújo, Eduardo Fonteshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787131J6Paula, Rinaldo Cesar dehttp://lattes.cnpq.br/18206261000810272015-03-26T12:27:08Z2012-03-282015-03-26T12:27:08Z2011-02-25FLORES, Andressa Vasconcelos. Germination of Melanoxylon brauna (SCHOTT) seeds under different temperatures: morphophysiologic and enzymatic aspects. 2011. 78 f. Tese (Doutorado em Manejo Florestal; Meio Ambiente e Conservação da Natureza; Silvicultura; Tecnologia e Utilização de) - Universidade Federal de Viçosa, Viçosa, 2011.http://locus.ufv.br/handle/123456789/558The germination of tropical tree species needs more precise information on the physical and intrinsic factors that may interfere in the germination process. Given this fact, this study aimed to investigate the morphometric characteristics and increasing levels of temperature and its implications on the physiology and metabolism of Melanoxylon brauna Schott seeds during the germination process. To this end, the experiments were conducted at the Laboratory of Seed Analysis of Forestry in Department of Forestry, University Federal of Viçosa - UFV. The seeds used in this study were collected from 10 trees in the city of Leopoldina - MG. In the first chapter, two assays were conducted, the first to evaluate the effect of color and size, in which the seeds were visually classified into light and dark, and in relation to size in large and small, and then were sown in two pieces of filter paper. In the second assay, were evaluated the effect of different substrates on germination. Thus, were tested the substrates on sand (SA), between sand (EA), on paper germitest (SP), between paper germitest (EP) and paper roll germitest (RP). In both trials, seeds were placed in BOD under constant temperature of 25°C and constant light for 10 days, and evaluated the characteristics of germination speed index (GSI) and percentage of germination. In the second chapter were carried out three assays. In the first were determined curves of soaking at temperatures of 5, 10, 15, 20, 25, 30, 35, 40 and 45°C. In the second trial, to identify the cardinal temperatures, seeds were kept under each temperature for a period of 10 days in BOD, under constant light. For the third test, was carried out electrical conductivity test (EC) with the seeds incubated for 24, 48 and 72 hours of immersion in each of the temperatures mentioned above, and then they were placed in erlenmeyers with 75 mL deionized water at 25°C, for 24 hours for subsequent reading of conductivity. In the third chapter, enzymatic analysis was performed. Measurements of the activities of superoxide dismutase (SOD, EC 1.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11) and catalase (CAT, EC 1.11.1.6) were made in the micropylar endosperm and embryonic axis of seeds germinated under the same conditions described above, at temperatures of 15, 25, 30 and 40°C, and every 12 hours until the samples were taken root protrusion of 50% of the seeds or 168 hours to a temperature of 15°C. Seeds small and light have lower germination e IVG that the small and large, regardless of color. The substrate most suitable for the testing laboratory germination is the paper roll germitest. The temperature appeared significantly in germination and synchronization of Melanoxylon brauna seeds. The cardinal temperatures for germination (%) are: 12.3°C - minimum 30.0°C - optimal (theoretical) and 42.7°C maximum. The temperature ranges considering all the variables investigated were: 12.1 to 12.6°C (minimum); 30 to 35.8 ° C (optimum) and 42.4 to 43°C (maximum). The specie has a wide range of germination with respect to temperature, germination was observed in the temperature range from 12 to 42°C. The EC test was efficient to evaluate the effect of temperature on the physiology and according to this, the optimum temperature is 27°C. Did not detect the activity of SOD, APX and CAT in the micropylar endosperm during seed germination under different temperatures. In the embryonic axis, the activities of SOD and CAT showed greater changes along the germination when subjected to temperatures of 15 and 40°C. The APX enzyme activity in the embryonic axis was not affected by temperature during germination and its activity was extremely low.A germinação de espécies arbóreas tropicais ainda carece de informações mais precisas sobre fatores físicos e as intrínsecas às sementes que podem interferir no processo germinativo dos mesmos. Diante deste fato, o presente trabalho teve por objetivo investigar as características morfométricas e níveis crescentes de temperatura e suas implicações sobre a fisiologia e o metabolismo de sementes de Melanoxylon brauna Schott durante o processo germinativo. Para tanto, os experimentos foram conduzidos no Laboratório de Análise de Sementes Florestais do Departamento de Engenharia Florestal da Universidade Federal de Viçosa - UFV. As sementes utilizadas neste trabalho foram colhidas em 10 árvores-matrizes na cidade de Leopoldina - MG. No primeiro capítulo, foram realizados dois ensaios, o primeiro para avaliar o efeito da cor e do tamanho, no qual as sementes foram classificadas visualmente em claras e escuras, e em relação ao tamanho em pequenas e grandes, e posteriormente, foram semeadas sob duas folhas de papel filtro. No segundo ensaio, foi avaliado o efeito de diferentes substratos na germinação. Para tanto, foram testados os substratos sobre areia (SA), entre areia (EA), sobre papel germitest (SP), entre papel germitest (EP) e rolo de papel germitest (RP). Em ambos os ensaios, as sementes foram colocadas em câmara BOD sob temperatura constante de 25°C e luz constante, por período de 10 dias, sendo avaliadas as características índice de velocidade de germinação (IVG) e a porcentagem de germinação. No segundo capítulo foram realizados três ensaios. No primeiro foram determinas das curvas de embebição para as temperaturas de: 5, 10, 15, 20, 25, 30, 35, 40 e 45 °C. No segundo ensaio, para identificar as temperaturas cardinais, as sementes foram mantidas sob cada temperatura, pelo período de 10 dias em câmara tipo BOD, sob luz constante. No terceiro ensaio, foi realizado teste de condutividade elétrica (CE) com as sementes incubadas por períodos de 24, 48 e 72 horas de embebição em cada uma das temperaturas citadas anteriormente, e, em seguida, as mesmas foram colocadas em erlenmeyers com 75 mL de água deionizada a 25°C, por 24 horas para posterior leitura da condutividade. No terceiro capítulo, foram realizadas análises enzimáticas. As determinações das atividades das enzimas superóxido dismutase (SOD, EC 1.15.1.1), ascorbato peroxidase (APX, EC 1.11.1.11) e catalase (CAT, EC 1.11.1.6) foram feitas no endosperma micropilar e no eixo embrionário de sementes colocadas para germinar sob as mesmas condições descritas acima, sob temperaturas de 15, 25, 30 e 40°C, e a cada 12 horas foram retiradas amostras até a protrusão radicular de 50% das sementes ou 168 horas para a temperatura de 15°C. Sementes pequenas e claras possuem germinação e IVG inferiores às sementes pequenas e escuras e às grandes, independentemente da cor. O substrato mais indicado para a realização de testes de germinação em laboratório é o rolo de papel germitest. A temperatura atuou de forma significativa na germinação e na sincronização de sementes de Melanoxylon brauna. As temperaturas cardinais para germinação (%) são: 12,3°C mínima, 30,0°C ótima (teórica) e 42,7°C máxima. As faixas de temperatura considerando-se todas as variáveis investigadas foram: 12,1-12,6°C (mínima); 30-35,8°C (ótima); e 42,4-43°C (máxima). A espécie apresenta uma grande amplitude de germinação com relação à temperatura, sendo verificada germinação na faixa de temperatura de 12 a 42°C. O teste de CE foi eficiente para avaliar o efeito da temperatura sobre a qualidade fisiológica e segundo este, a temperatura ótima é de 27°C. Não foi detectada a atividade das enzimas SOD, APX e CAT no endosperma micropilar durante a germinação de sementes sob diferentes temperaturas. No eixo embrionário, as atividades das enzimas SOD e CAT apresentaram maiores alterações ao longo da germinação quando submetidas às temperaturas de 15 e 40°C. A atividade da enzima APX, no eixo embrionário, não foi afetada pela temperatura ao longo da germinação e sua atividade foi extremamente baixa.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de ViçosaDoutorado em Ciência FlorestalUFVBRManejo Florestal; Meio Ambiente e Conservação da Natureza; Silvicultura; Tecnologia e Utilização deCondutividade elétricaEspécies arbóreasRadicais livresDeteriorizaçãoProteínaElectrical conductivityTree speciesFree radicalsDeteriorationProteinCNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL::SILVICULTURAGerminação de sementes de Melanoxylon brauna (SCHOTT) sob diferentes temperaturas: aspectos morfofisiológicos e enzimáticosGermination of Melanoxylon brauna (SCHOTT) seeds under different temperatures: morphophysiologic and enzymatic aspectsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf3622873https://locus.ufv.br//bitstream/123456789/558/1/texto%20completo.pdff235f2f093ccd1cff781fb10f32a4a8eMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain159263https://locus.ufv.br//bitstream/123456789/558/2/texto%20completo.pdf.txtbc4f304547557a0b9a48a01893ba58cbMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3640https://locus.ufv.br//bitstream/123456789/558/3/texto%20completo.pdf.jpg7720749167f66715e5808dbb74bdf8c5MD53123456789/5582016-04-06 23:07:09.838oai:locus.ufv.br:123456789/558Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-07T02:07:09LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Germinação de sementes de Melanoxylon brauna (SCHOTT) sob diferentes temperaturas: aspectos morfofisiológicos e enzimáticos
dc.title.alternative.eng.fl_str_mv Germination of Melanoxylon brauna (SCHOTT) seeds under different temperatures: morphophysiologic and enzymatic aspects
title Germinação de sementes de Melanoxylon brauna (SCHOTT) sob diferentes temperaturas: aspectos morfofisiológicos e enzimáticos
spellingShingle Germinação de sementes de Melanoxylon brauna (SCHOTT) sob diferentes temperaturas: aspectos morfofisiológicos e enzimáticos
Flores, Andressa Vasconcelos
Condutividade elétrica
Espécies arbóreas
Radicais livres
Deteriorização
Proteína
Electrical conductivity
Tree species
Free radicals
Deterioration
Protein
CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL::SILVICULTURA
title_short Germinação de sementes de Melanoxylon brauna (SCHOTT) sob diferentes temperaturas: aspectos morfofisiológicos e enzimáticos
title_full Germinação de sementes de Melanoxylon brauna (SCHOTT) sob diferentes temperaturas: aspectos morfofisiológicos e enzimáticos
title_fullStr Germinação de sementes de Melanoxylon brauna (SCHOTT) sob diferentes temperaturas: aspectos morfofisiológicos e enzimáticos
title_full_unstemmed Germinação de sementes de Melanoxylon brauna (SCHOTT) sob diferentes temperaturas: aspectos morfofisiológicos e enzimáticos
title_sort Germinação de sementes de Melanoxylon brauna (SCHOTT) sob diferentes temperaturas: aspectos morfofisiológicos e enzimáticos
author Flores, Andressa Vasconcelos
author_facet Flores, Andressa Vasconcelos
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/1039066389051551
dc.contributor.author.fl_str_mv Flores, Andressa Vasconcelos
dc.contributor.advisor-co1.fl_str_mv Guimarães, Valéria Monteze
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798758T3
dc.contributor.advisor-co2.fl_str_mv Gonçalves, José Francisco de Carvalho
dc.contributor.advisor-co2Lattes.fl_str_mv http://lattes.cnpq.br/0553096006639259
dc.contributor.advisor1.fl_str_mv Borges, Eduardo Euclydes de Lima e
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787799U8
dc.contributor.referee1.fl_str_mv Araújo, Eduardo Fontes
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787131J6
dc.contributor.referee2.fl_str_mv Paula, Rinaldo Cesar de
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/1820626100081027
contributor_str_mv Guimarães, Valéria Monteze
Gonçalves, José Francisco de Carvalho
Borges, Eduardo Euclydes de Lima e
Araújo, Eduardo Fontes
Paula, Rinaldo Cesar de
dc.subject.por.fl_str_mv Condutividade elétrica
Espécies arbóreas
Radicais livres
Deteriorização
Proteína
topic Condutividade elétrica
Espécies arbóreas
Radicais livres
Deteriorização
Proteína
Electrical conductivity
Tree species
Free radicals
Deterioration
Protein
CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL::SILVICULTURA
dc.subject.eng.fl_str_mv Electrical conductivity
Tree species
Free radicals
Deterioration
Protein
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL::SILVICULTURA
description The germination of tropical tree species needs more precise information on the physical and intrinsic factors that may interfere in the germination process. Given this fact, this study aimed to investigate the morphometric characteristics and increasing levels of temperature and its implications on the physiology and metabolism of Melanoxylon brauna Schott seeds during the germination process. To this end, the experiments were conducted at the Laboratory of Seed Analysis of Forestry in Department of Forestry, University Federal of Viçosa - UFV. The seeds used in this study were collected from 10 trees in the city of Leopoldina - MG. In the first chapter, two assays were conducted, the first to evaluate the effect of color and size, in which the seeds were visually classified into light and dark, and in relation to size in large and small, and then were sown in two pieces of filter paper. In the second assay, were evaluated the effect of different substrates on germination. Thus, were tested the substrates on sand (SA), between sand (EA), on paper germitest (SP), between paper germitest (EP) and paper roll germitest (RP). In both trials, seeds were placed in BOD under constant temperature of 25°C and constant light for 10 days, and evaluated the characteristics of germination speed index (GSI) and percentage of germination. In the second chapter were carried out three assays. In the first were determined curves of soaking at temperatures of 5, 10, 15, 20, 25, 30, 35, 40 and 45°C. In the second trial, to identify the cardinal temperatures, seeds were kept under each temperature for a period of 10 days in BOD, under constant light. For the third test, was carried out electrical conductivity test (EC) with the seeds incubated for 24, 48 and 72 hours of immersion in each of the temperatures mentioned above, and then they were placed in erlenmeyers with 75 mL deionized water at 25°C, for 24 hours for subsequent reading of conductivity. In the third chapter, enzymatic analysis was performed. Measurements of the activities of superoxide dismutase (SOD, EC 1.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11) and catalase (CAT, EC 1.11.1.6) were made in the micropylar endosperm and embryonic axis of seeds germinated under the same conditions described above, at temperatures of 15, 25, 30 and 40°C, and every 12 hours until the samples were taken root protrusion of 50% of the seeds or 168 hours to a temperature of 15°C. Seeds small and light have lower germination e IVG that the small and large, regardless of color. The substrate most suitable for the testing laboratory germination is the paper roll germitest. The temperature appeared significantly in germination and synchronization of Melanoxylon brauna seeds. The cardinal temperatures for germination (%) are: 12.3°C - minimum 30.0°C - optimal (theoretical) and 42.7°C maximum. The temperature ranges considering all the variables investigated were: 12.1 to 12.6°C (minimum); 30 to 35.8 ° C (optimum) and 42.4 to 43°C (maximum). The specie has a wide range of germination with respect to temperature, germination was observed in the temperature range from 12 to 42°C. The EC test was efficient to evaluate the effect of temperature on the physiology and according to this, the optimum temperature is 27°C. Did not detect the activity of SOD, APX and CAT in the micropylar endosperm during seed germination under different temperatures. In the embryonic axis, the activities of SOD and CAT showed greater changes along the germination when subjected to temperatures of 15 and 40°C. The APX enzyme activity in the embryonic axis was not affected by temperature during germination and its activity was extremely low.
publishDate 2011
dc.date.issued.fl_str_mv 2011-02-25
dc.date.available.fl_str_mv 2012-03-28
2015-03-26T12:27:08Z
dc.date.accessioned.fl_str_mv 2015-03-26T12:27:08Z
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dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
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dc.identifier.citation.fl_str_mv FLORES, Andressa Vasconcelos. Germination of Melanoxylon brauna (SCHOTT) seeds under different temperatures: morphophysiologic and enzymatic aspects. 2011. 78 f. Tese (Doutorado em Manejo Florestal; Meio Ambiente e Conservação da Natureza; Silvicultura; Tecnologia e Utilização de) - Universidade Federal de Viçosa, Viçosa, 2011.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/558
identifier_str_mv FLORES, Andressa Vasconcelos. Germination of Melanoxylon brauna (SCHOTT) seeds under different temperatures: morphophysiologic and enzymatic aspects. 2011. 78 f. Tese (Doutorado em Manejo Florestal; Meio Ambiente e Conservação da Natureza; Silvicultura; Tecnologia e Utilização de) - Universidade Federal de Viçosa, Viçosa, 2011.
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dc.publisher.department.fl_str_mv Manejo Florestal; Meio Ambiente e Conservação da Natureza; Silvicultura; Tecnologia e Utilização de
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