Biogeografia histórica do lambari de ampla distribuição Astyanax aff. bimaculatus (Teleostei: Characidae) no sudeste brasileiro, com base em padrões de variação citogenéticos e moleculares
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/4810 |
Resumo: | The species of the genus Astyanax are widely distributed in the Neotropical region and have few morphological, ecological and behavioral differences, which complicates the taxonomic classification of this taxon. The nominal species Astyanax bimaculatus was described by Linnaeus in 1758 and currently species with two oval spots that occur in several Neotropical basins are considered as belonging to bimaculatus complex. The objective of this study was to determine the cytogenetic and molecular characteristics (mitochondrial DNA) of 19 populations currently assigned to Astyanax bimaculatus from complex coastal basins of southeastern Minas Gerais using conventional staining, nucleolar organizer regions Ag-NORs, C-band, DAPI, FISH and the gene cytochrome oxidase I - COI. Data were compared with other species of the complex. The karyotype 6m +20 sm +18 st +6 t occurred in all populations, with Ag-NORs ranging from two to eight stained chromosomes. The C-banding showed centromeric and pericentromeric markings, evidenced by the fluorochrome DAPI. Fluorescent 18S and 5S rDNA probes marked one chromosome pair respectively. The phylogeny obtained with the COI gene indicated the presence of two haplogroups with large molecular distance between them: haplogroup I was more closely related to the populations of the São Francisco River Basin, while haplogroup II was subdivided into a group which was more related to populations from the upper Paraná (IIA) and another group formed exclusively by coastal haplotypes (IIB). The karyotype uniformity of coastal populations is greater than the recognized Astyanax altiparanae, suggesting the persistence of large populations. Polymorphisms involving C-band patterns indicate that the addition and reduction of heterochromatin blocks are compatible with intraspecific variation. The molecular data suggest that coastal basins received migrants from continental basins, with more recent geodispersion of a coastal haplogroup, probably related to the dynamic confluence of coastal basins during glacial periods. The genetic similarity between São Francisco River Basin and coastal basins indicates that the nominal species Astyanax lacustris does not represent a valid taxon. While the Upper Paraná Basin maintained a molecular identity consistent with the existence of the nominal taxon Astyanax altiparanae that, agreeing with the cytogenetic data, also occurs in the Paraíba do Sul River Basin. It is concluded that the bimaculatus complex in southeastern Brazil is composed by three lineages, including only two species Astyanax aff. bimaculatus present in São Francisco and coastal basins and A. altiparanae present in the Upper Paraná. |
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Cunha, Marina Souza dahttp://lattes.cnpq.br/0222235511376911Campos, Lúcio Antonio de Oliveirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783908P9Santos, Jorge Abdala Dergam doshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780131D9Gonçalves, Reggiani Vilelahttp://lattes.cnpq.br/26195656662775322015-03-26T13:42:34Z2015-02-232015-03-26T13:42:34Z2014-07-30CUNHA, Marina Souza da. Historial biogeography of the wide distribution lambari Astyanax aff. bimaculatus (Teleostei: Characidae) on southeastern Brazil, based on cytogenetic and molecular data. 2014. 67 f. Dissertação (Mestrado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2014.http://locus.ufv.br/handle/123456789/4810The species of the genus Astyanax are widely distributed in the Neotropical region and have few morphological, ecological and behavioral differences, which complicates the taxonomic classification of this taxon. The nominal species Astyanax bimaculatus was described by Linnaeus in 1758 and currently species with two oval spots that occur in several Neotropical basins are considered as belonging to bimaculatus complex. The objective of this study was to determine the cytogenetic and molecular characteristics (mitochondrial DNA) of 19 populations currently assigned to Astyanax bimaculatus from complex coastal basins of southeastern Minas Gerais using conventional staining, nucleolar organizer regions Ag-NORs, C-band, DAPI, FISH and the gene cytochrome oxidase I - COI. Data were compared with other species of the complex. The karyotype 6m +20 sm +18 st +6 t occurred in all populations, with Ag-NORs ranging from two to eight stained chromosomes. The C-banding showed centromeric and pericentromeric markings, evidenced by the fluorochrome DAPI. Fluorescent 18S and 5S rDNA probes marked one chromosome pair respectively. The phylogeny obtained with the COI gene indicated the presence of two haplogroups with large molecular distance between them: haplogroup I was more closely related to the populations of the São Francisco River Basin, while haplogroup II was subdivided into a group which was more related to populations from the upper Paraná (IIA) and another group formed exclusively by coastal haplotypes (IIB). The karyotype uniformity of coastal populations is greater than the recognized Astyanax altiparanae, suggesting the persistence of large populations. Polymorphisms involving C-band patterns indicate that the addition and reduction of heterochromatin blocks are compatible with intraspecific variation. The molecular data suggest that coastal basins received migrants from continental basins, with more recent geodispersion of a coastal haplogroup, probably related to the dynamic confluence of coastal basins during glacial periods. The genetic similarity between São Francisco River Basin and coastal basins indicates that the nominal species Astyanax lacustris does not represent a valid taxon. While the Upper Paraná Basin maintained a molecular identity consistent with the existence of the nominal taxon Astyanax altiparanae that, agreeing with the cytogenetic data, also occurs in the Paraíba do Sul River Basin. It is concluded that the bimaculatus complex in southeastern Brazil is composed by three lineages, including only two species Astyanax aff. bimaculatus present in São Francisco and coastal basins and A. altiparanae present in the Upper Paraná.As espécies do gênero Astyanax apresentam ampla distribuição na região neotropical e apresentam poucas diferenças morfológicas, ecológicas e comportamentais, o que dificulta a classificação taxonômica desse táxon. A espécie nominal Astyanax bimaculatus foi descrita em 1758 por Linnaeus e, atualmente, espécies com duas manchas ovaladas que ocorrem em diversas bacias neotropicais são consideradas como pertencentes ao complexo bimaculatus. O objetivo deste trabalho foi determinar as características citogenéticas e moleculares (DNA mitocondrial) de 19 populações consideradas como pertencentes ao complexo Astyanax bimaculatus das bacias costeiras do sudeste de Minas Gerais utilizando coloração convencional, regiões organizadoras de nucléolos Ag-NOrs, banda C, DAPI, FISH e o gene citocromo oxidase I - COI. Os dados foram comparados com outras espécies do complexo. O cariótipo 6m+20sm+18st+6t ocorreu em todas as populações, com Ag-NORs variando de dois a oito cromossomos marcados. A banda C mostrou marcações centroméricas e pericentroméricas, evidenciadas pelo fluorocromo DAPI. As sondas 18S e 5S marcaram apenas um par cromossômico respectivamente. A filogenia obtida com o gene COI indicou a presença de dois haplogrupos com grande distância molecular: o haplogrupo I mais aparentado com populações da bacia do rio São Francisco, enquanto o haplogrupo II subdividiu-se em um grupo mais aparentado com populações do alto Paraná (IIA) e outro grupo formado exclusivamente por haplótipos costeiros (IIB). A uniformidade cariotípica das populações costeiras é maior que a reconhecida em Astyanax altiparanae, sugerindo a persistência de grandes populações. Polimorfismos envolvendo padrões de banda C indicam que a adição e redução de blocos de heterocromatina são consideradas variações intraespecíficas. Os dados moleculares sugerem que as bacias costeiras receberam aporte de bacias continentais, com geodispersão mais recente de um haplogrupo costeiro, provavelmente relacionado com a dinâmica de confluência de bacias costeiras durante os períodos glaciais. A similaridade genética entre a bacia do rio São Francisco e as bacias costeiras indica que a espécie nominal Astyanax lacustris não representa um táxon válido. Enquanto a bacia do alto Paraná manteve uma identidade molecular condizente com a existência do táxon nominal Astyanax altiparanae que, condizente com os dados citogenéticos, também ocorre na bacia do rio Paraíba do Sul. Conclui-se que o complexo bimaculatus no sudeste brasileiro é composto de três linhagens, englobando apenas duas espécies: Astyanax aff. bimaculatus presente no São Francisco e nas bacias costeiras e A. altiparanae presente no alto Paraná.Fundação de Amparo a Pesquisa do Estado de Minas Geraisapplication/pdfporUniversidade Federal de ViçosaMestrado em Genética e MelhoramentoUFVBRGenética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; MeGenética animalLambari (Peixe) - CitogenéticaLambari (Peixe) - CitogenéticaLambari (Peixe) - MorfologiaAstyanax bimaculatusAstyanax lacustrisBrasil - SudesteAnimal geneticsTetra (Fish) - CytogeneticsTetra (Fish) - CytogeneticsTetra (Fish) - MorphologyAstyanax bimaculatusAstyanax lacustrisBrazil - South eastCNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA ANIMALBiogeografia histórica do lambari de ampla distribuição Astyanax aff. bimaculatus (Teleostei: Characidae) no sudeste brasileiro, com base em padrões de variação citogenéticos e molecularesHistorial biogeography of the wide distribution lambari Astyanax aff. bimaculatus (Teleostei: Characidae) on southeastern Brazil, based on cytogenetic and molecular datainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf1695521https://locus.ufv.br//bitstream/123456789/4810/1/texto%20completo.pdf9a0915b224b9fc02033cedd8cfc42515MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain100860https://locus.ufv.br//bitstream/123456789/4810/2/texto%20completo.pdf.txt6484b2333afd3782e015e8e13eedd818MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3618https://locus.ufv.br//bitstream/123456789/4810/3/texto%20completo.pdf.jpgf272d11aa45db9a62c31a08e1be40f63MD53123456789/48102016-04-10 23:16:46.037oai:locus.ufv.br:123456789/4810Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:16:46LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Biogeografia histórica do lambari de ampla distribuição Astyanax aff. bimaculatus (Teleostei: Characidae) no sudeste brasileiro, com base em padrões de variação citogenéticos e moleculares |
dc.title.alternative.eng.fl_str_mv |
Historial biogeography of the wide distribution lambari Astyanax aff. bimaculatus (Teleostei: Characidae) on southeastern Brazil, based on cytogenetic and molecular data |
title |
Biogeografia histórica do lambari de ampla distribuição Astyanax aff. bimaculatus (Teleostei: Characidae) no sudeste brasileiro, com base em padrões de variação citogenéticos e moleculares |
spellingShingle |
Biogeografia histórica do lambari de ampla distribuição Astyanax aff. bimaculatus (Teleostei: Characidae) no sudeste brasileiro, com base em padrões de variação citogenéticos e moleculares Cunha, Marina Souza da Genética animal Lambari (Peixe) - Citogenética Lambari (Peixe) - Citogenética Lambari (Peixe) - Morfologia Astyanax bimaculatus Astyanax lacustris Brasil - Sudeste Animal genetics Tetra (Fish) - Cytogenetics Tetra (Fish) - Cytogenetics Tetra (Fish) - Morphology Astyanax bimaculatus Astyanax lacustris Brazil - South east CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA ANIMAL |
title_short |
Biogeografia histórica do lambari de ampla distribuição Astyanax aff. bimaculatus (Teleostei: Characidae) no sudeste brasileiro, com base em padrões de variação citogenéticos e moleculares |
title_full |
Biogeografia histórica do lambari de ampla distribuição Astyanax aff. bimaculatus (Teleostei: Characidae) no sudeste brasileiro, com base em padrões de variação citogenéticos e moleculares |
title_fullStr |
Biogeografia histórica do lambari de ampla distribuição Astyanax aff. bimaculatus (Teleostei: Characidae) no sudeste brasileiro, com base em padrões de variação citogenéticos e moleculares |
title_full_unstemmed |
Biogeografia histórica do lambari de ampla distribuição Astyanax aff. bimaculatus (Teleostei: Characidae) no sudeste brasileiro, com base em padrões de variação citogenéticos e moleculares |
title_sort |
Biogeografia histórica do lambari de ampla distribuição Astyanax aff. bimaculatus (Teleostei: Characidae) no sudeste brasileiro, com base em padrões de variação citogenéticos e moleculares |
author |
Cunha, Marina Souza da |
author_facet |
Cunha, Marina Souza da |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/0222235511376911 |
dc.contributor.author.fl_str_mv |
Cunha, Marina Souza da |
dc.contributor.advisor-co1.fl_str_mv |
Campos, Lúcio Antonio de Oliveira |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783908P9 |
dc.contributor.advisor1.fl_str_mv |
Santos, Jorge Abdala Dergam dos |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780131D9 |
dc.contributor.referee1.fl_str_mv |
Gonçalves, Reggiani Vilela |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/2619565666277532 |
contributor_str_mv |
Campos, Lúcio Antonio de Oliveira Santos, Jorge Abdala Dergam dos Gonçalves, Reggiani Vilela |
dc.subject.por.fl_str_mv |
Genética animal Lambari (Peixe) - Citogenética Lambari (Peixe) - Citogenética Lambari (Peixe) - Morfologia Astyanax bimaculatus Astyanax lacustris Brasil - Sudeste |
topic |
Genética animal Lambari (Peixe) - Citogenética Lambari (Peixe) - Citogenética Lambari (Peixe) - Morfologia Astyanax bimaculatus Astyanax lacustris Brasil - Sudeste Animal genetics Tetra (Fish) - Cytogenetics Tetra (Fish) - Cytogenetics Tetra (Fish) - Morphology Astyanax bimaculatus Astyanax lacustris Brazil - South east CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA ANIMAL |
dc.subject.eng.fl_str_mv |
Animal genetics Tetra (Fish) - Cytogenetics Tetra (Fish) - Cytogenetics Tetra (Fish) - Morphology Astyanax bimaculatus Astyanax lacustris Brazil - South east |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA ANIMAL |
description |
The species of the genus Astyanax are widely distributed in the Neotropical region and have few morphological, ecological and behavioral differences, which complicates the taxonomic classification of this taxon. The nominal species Astyanax bimaculatus was described by Linnaeus in 1758 and currently species with two oval spots that occur in several Neotropical basins are considered as belonging to bimaculatus complex. The objective of this study was to determine the cytogenetic and molecular characteristics (mitochondrial DNA) of 19 populations currently assigned to Astyanax bimaculatus from complex coastal basins of southeastern Minas Gerais using conventional staining, nucleolar organizer regions Ag-NORs, C-band, DAPI, FISH and the gene cytochrome oxidase I - COI. Data were compared with other species of the complex. The karyotype 6m +20 sm +18 st +6 t occurred in all populations, with Ag-NORs ranging from two to eight stained chromosomes. The C-banding showed centromeric and pericentromeric markings, evidenced by the fluorochrome DAPI. Fluorescent 18S and 5S rDNA probes marked one chromosome pair respectively. The phylogeny obtained with the COI gene indicated the presence of two haplogroups with large molecular distance between them: haplogroup I was more closely related to the populations of the São Francisco River Basin, while haplogroup II was subdivided into a group which was more related to populations from the upper Paraná (IIA) and another group formed exclusively by coastal haplotypes (IIB). The karyotype uniformity of coastal populations is greater than the recognized Astyanax altiparanae, suggesting the persistence of large populations. Polymorphisms involving C-band patterns indicate that the addition and reduction of heterochromatin blocks are compatible with intraspecific variation. The molecular data suggest that coastal basins received migrants from continental basins, with more recent geodispersion of a coastal haplogroup, probably related to the dynamic confluence of coastal basins during glacial periods. The genetic similarity between São Francisco River Basin and coastal basins indicates that the nominal species Astyanax lacustris does not represent a valid taxon. While the Upper Paraná Basin maintained a molecular identity consistent with the existence of the nominal taxon Astyanax altiparanae that, agreeing with the cytogenetic data, also occurs in the Paraíba do Sul River Basin. It is concluded that the bimaculatus complex in southeastern Brazil is composed by three lineages, including only two species Astyanax aff. bimaculatus present in São Francisco and coastal basins and A. altiparanae present in the Upper Paraná. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-07-30 |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:42:34Z |
dc.date.available.fl_str_mv |
2015-02-23 2015-03-26T13:42:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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dc.identifier.citation.fl_str_mv |
CUNHA, Marina Souza da. Historial biogeography of the wide distribution lambari Astyanax aff. bimaculatus (Teleostei: Characidae) on southeastern Brazil, based on cytogenetic and molecular data. 2014. 67 f. Dissertação (Mestrado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2014. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/4810 |
identifier_str_mv |
CUNHA, Marina Souza da. Historial biogeography of the wide distribution lambari Astyanax aff. bimaculatus (Teleostei: Characidae) on southeastern Brazil, based on cytogenetic and molecular data. 2014. 67 f. Dissertação (Mestrado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2014. |
url |
http://locus.ufv.br/handle/123456789/4810 |
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Universidade Federal de Viçosa |
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Mestrado em Genética e Melhoramento |
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UFV |
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BR |
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Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me |
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Universidade Federal de Viçosa |
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