Influência do tempo e temperatura sobre a estabilidade de constituintes do soro e plasma sangüíneos de eqüinos Mangalarga Marchador

• Autor(a) principal: Comis, Miriam Bordinhão
• Data de Publicação: 2006
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/4961
• topic: Tempo; Temperatura; Eqüinos; Time; Temperature; Horse; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL::CLINICA VETERINARIA

The study of time and temperature influences on stability of serum and plasma constituents has deserved prominence on research due its great importance as source of diagnosis and prognosis of several diseases, as well due to a limited number of studies involving these effects on biochemical constituents of horses. Possible alterations on the activity of serum and plasma constituents were verified on 10 adults and clinically healthy MM horses (Mangalarga Marchador). Samples were submitted to the following storage methods: room temperature (16.6 to 29.6°C), cooling (5°C) and freezing (-20°C and -76 °C. Laboratorial analysis were performed in 11 different times: time zero immediately after sample collection, 12, 24, 36, 48 hours after; and 7, 15, 30, 60, 90 and 180 days after collection of samples. The constituents determined were albumin, sodium, potassium, chloride (Cl), creatinine, urea, total bilirubin, magnesium, alkaline fosfatase (FA), gammaglutamyl transferase (GGT), aspartate aminotransferase (AST), creatine kinase (CK), lactate dehydrogenase (LDH), total plasma protein (PPT), fibrinogen, glucose and lactate. Results obtained on samples at room temperature (16.6 to 29.6°C) revealed a decrease on values found in the following blood constituents: albumin (12, 24 and 36 hours), lactate (48 hours) and FA (48 hours), in the same group it had increased values on fibrinogen (12, 24 and 36 hours) and sodium (36 hours). Samples at cooling (5°C) presented decreased values on albumin (7 and 15 days), potassium (15 and 30 days), AST (15 and 30 days) and LDH (15 and 30 days), and increased values on total bilirubin (30 days) and urea (30 days). Samples freezed at -20 °C showed decreased values on the following constituents: PPT (60 and 90 days), Cl (90 days), FA (90 and 180 days) and LDH (90 and 180 days); and increased values on PPT (30 days), Cl (30 days) and creatinine (90 days). Samples freezed at -76°C presented decreased values of albumin (180 days), Cl (90 days), creatinine (180 days) and FA (90 and 180 days); increased values of PPT (30 and 60 days), Cl (30 days) and FA (60 days). Significative alterations occurred on the blood constituents studied at all times and temperatures with exception of glucose, magnesium, GGT and CK. Despite the observed alterations, stability of the variables prevailed during all experimental period, assenting the conclusion that each constituent has its proper time and temperature for storage.