Viabilidade de oósporos oriundos de cruzamentos de isolados brasileiros de Phytophthora infestans
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/4426 |
Resumo: | Phytophthora infestans isolates were obtained from infected potato or tomato leaves and tomato fruits collected from the South, Southeast, Northeast, and Central-West regions in Brazil. Eleven isolates of the A1 mating type and four of the A2 were paired in culture media to assess production of oospores. After quantifying the number of oospores obtained from each pairing, two variables, number of oospores produced in culture medium (NOCM) and number of oospores extracted from the medium (NOEXT), were subjected to a partial diallel analysis with F1 s only, in order to estimate the fecundity of the crosses. Oospore viability was estimated with a plasmolysis in NaCl test and germ tube formation was determined in water agar. All crosses produced oospores. The number of oospores varied among the crosses and ranged from 233 to 3900/ml. The general combination ability (GCA) and specific combination ability (SCA) varied among isolates and were significant (P < 0.0001). For both NOCM and NOEXT the A2 mating type isolate with the highest GCA value was Pib-750. Among the A1 mating type isolates, highest GCA values were estimated for Pib-754 and Pib-742, based on the NOCM and NOEXT, respectively. Based on SCA, the best isolate combination for oospores production was between Pib-731 x Pib-755, for both variables. There was no clear trend regarding a possible geographical proximity effect on the fecundity of crosses. Although the crosses produced oospores, viability and germination rates were low, on average 0.3% to 0.03%, respectively. Despite the low viability, production of oospores can be a threat to potato and tomato crops as a source of genetic diversity for this pathogen. |
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Cruzado, Zoila Rosa Magnolia Santa Cruzhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4745445Y6Barreto, Robert Weingarthttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783300H6Maffia, Luiz Antôniohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783229P9Teixeira, Hudsonhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4791813Z1Pereira, Olinto Liparinihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767879D4Paula Júnior, Trazilbo José dehttp://lattes.cnpq.br/78992760970188762015-03-26T13:37:52Z2006-11-072015-03-26T13:37:52Z2006-08-31CRUZADO, Zoila Rosa Magnolia Santa Cruz. Viability of oospores originated from crosses of Brazilian isolates of Phytophthora infestans. 2006. 34 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2006.http://locus.ufv.br/handle/123456789/4426Phytophthora infestans isolates were obtained from infected potato or tomato leaves and tomato fruits collected from the South, Southeast, Northeast, and Central-West regions in Brazil. Eleven isolates of the A1 mating type and four of the A2 were paired in culture media to assess production of oospores. After quantifying the number of oospores obtained from each pairing, two variables, number of oospores produced in culture medium (NOCM) and number of oospores extracted from the medium (NOEXT), were subjected to a partial diallel analysis with F1 s only, in order to estimate the fecundity of the crosses. Oospore viability was estimated with a plasmolysis in NaCl test and germ tube formation was determined in water agar. All crosses produced oospores. The number of oospores varied among the crosses and ranged from 233 to 3900/ml. The general combination ability (GCA) and specific combination ability (SCA) varied among isolates and were significant (P < 0.0001). For both NOCM and NOEXT the A2 mating type isolate with the highest GCA value was Pib-750. Among the A1 mating type isolates, highest GCA values were estimated for Pib-754 and Pib-742, based on the NOCM and NOEXT, respectively. Based on SCA, the best isolate combination for oospores production was between Pib-731 x Pib-755, for both variables. There was no clear trend regarding a possible geographical proximity effect on the fecundity of crosses. Although the crosses produced oospores, viability and germination rates were low, on average 0.3% to 0.03%, respectively. Despite the low viability, production of oospores can be a threat to potato and tomato crops as a source of genetic diversity for this pathogen.Isolados de Phytophthora infestans foram obtidos de folhas de batata e tomate e frutos de tomate coletados das regiões Sul, Sudeste, Nordeste e Centro Oeste do Brasil. Onze isolados do grupo de compatibilidade A1 e quatro do A2 foram pareados em meio de cultura, para avaliar a produção de oósporos. Após quantificação de oósporos resultantes de cada pareamento, duas variáveis, número de oósporos determinado em meio de cultura (NOMC) e número de oósporos extraídos do meio (NOEXT), foram submetidas à análise de dialelos parciais, com apenas F1, para estimar possíveis diferenças quanto à fecundidade dos cruzamentos realizados. Avaliaram-se a viabilidade e germinação in vitro de oósporos extraídos do meio de cultura. Avaliou-se a viabilidade por meio do teste de plasmólise em NaCl e a produção de tubo germinativo foi determinada em ágar-água. Em todos os cruzamentos houve produção de oósporos. O número de oósporos variou de 233 a 3900/ml. As capacidades geral (CGC) e específica (CEC) de combinação variaram entre os isolados e foram significativas (P < 0,0001). Para NOMC e NOEXT, o isolado do grupo de compatibilidade A2 com maior valor de CGC foi o Pib-750. Do grupo A1, os valores mais altos de CGC foram para os isolados Pib-754 e Pib-742, com base em NOMC e NOEXT, respectivamente. Baseados nos valores de CEC, a combinação mais adequada para produzir oósporos foi Pib-731 x Pib-755, para ambas as variáveis. Não houve tendência efeito de proximidade geográfica sobre a fecundidade dos cruzamentos. Embora os cruzamentos tenham produzido oósporos, suas taxas de viabilidade e germinação foram baixas, em média, 0,3% e 0,03%, respectivamente. Apesar da baixa viabilidade, a produção de oósporos pode constituir-se em ameaça para as culturas de batata e tomate como fonte de variabilidade genética de P. infestans.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de ViçosaMestrado em FitopatologiaUFVBREtiologia; Epidemiologia; ControlePhytophthora infestansOósporosRequeimaGerminaçãoPhytophthora infestansOosporesGerminationCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIAViabilidade de oósporos oriundos de cruzamentos de isolados brasileiros de Phytophthora infestansViability of oospores originated from crosses of Brazilian isolates of Phytophthora infestansinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf376920https://locus.ufv.br//bitstream/123456789/4426/1/texto%20completo.pdf4d53b2d9d2463948a02bb78e95aa3950MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain55251https://locus.ufv.br//bitstream/123456789/4426/2/texto%20completo.pdf.txte16d2a3f63f5e19c2025f41e909a79ddMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3909https://locus.ufv.br//bitstream/123456789/4426/3/texto%20completo.pdf.jpgbfac66238ce990f55f03707c940b41b7MD53123456789/44262016-04-10 23:08:47.88oai:locus.ufv.br:123456789/4426Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:08:47LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Viabilidade de oósporos oriundos de cruzamentos de isolados brasileiros de Phytophthora infestans |
dc.title.alternative.eng.fl_str_mv |
Viability of oospores originated from crosses of Brazilian isolates of Phytophthora infestans |
title |
Viabilidade de oósporos oriundos de cruzamentos de isolados brasileiros de Phytophthora infestans |
spellingShingle |
Viabilidade de oósporos oriundos de cruzamentos de isolados brasileiros de Phytophthora infestans Cruzado, Zoila Rosa Magnolia Santa Cruz Phytophthora infestans Oósporos Requeima Germinação Phytophthora infestans Oospores Germination CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA |
title_short |
Viabilidade de oósporos oriundos de cruzamentos de isolados brasileiros de Phytophthora infestans |
title_full |
Viabilidade de oósporos oriundos de cruzamentos de isolados brasileiros de Phytophthora infestans |
title_fullStr |
Viabilidade de oósporos oriundos de cruzamentos de isolados brasileiros de Phytophthora infestans |
title_full_unstemmed |
Viabilidade de oósporos oriundos de cruzamentos de isolados brasileiros de Phytophthora infestans |
title_sort |
Viabilidade de oósporos oriundos de cruzamentos de isolados brasileiros de Phytophthora infestans |
author |
Cruzado, Zoila Rosa Magnolia Santa Cruz |
author_facet |
Cruzado, Zoila Rosa Magnolia Santa Cruz |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4745445Y6 |
dc.contributor.author.fl_str_mv |
Cruzado, Zoila Rosa Magnolia Santa Cruz |
dc.contributor.advisor-co1.fl_str_mv |
Barreto, Robert Weingart |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783300H6 |
dc.contributor.advisor1.fl_str_mv |
Maffia, Luiz Antônio |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783229P9 |
dc.contributor.referee1.fl_str_mv |
Teixeira, Hudson |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4791813Z1 |
dc.contributor.referee2.fl_str_mv |
Pereira, Olinto Liparini |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767879D4 |
dc.contributor.referee3.fl_str_mv |
Paula Júnior, Trazilbo José de |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/7899276097018876 |
contributor_str_mv |
Barreto, Robert Weingart Maffia, Luiz Antônio Teixeira, Hudson Pereira, Olinto Liparini Paula Júnior, Trazilbo José de |
dc.subject.por.fl_str_mv |
Phytophthora infestans Oósporos Requeima Germinação |
topic |
Phytophthora infestans Oósporos Requeima Germinação Phytophthora infestans Oospores Germination CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA |
dc.subject.eng.fl_str_mv |
Phytophthora infestans Oospores Germination |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA |
description |
Phytophthora infestans isolates were obtained from infected potato or tomato leaves and tomato fruits collected from the South, Southeast, Northeast, and Central-West regions in Brazil. Eleven isolates of the A1 mating type and four of the A2 were paired in culture media to assess production of oospores. After quantifying the number of oospores obtained from each pairing, two variables, number of oospores produced in culture medium (NOCM) and number of oospores extracted from the medium (NOEXT), were subjected to a partial diallel analysis with F1 s only, in order to estimate the fecundity of the crosses. Oospore viability was estimated with a plasmolysis in NaCl test and germ tube formation was determined in water agar. All crosses produced oospores. The number of oospores varied among the crosses and ranged from 233 to 3900/ml. The general combination ability (GCA) and specific combination ability (SCA) varied among isolates and were significant (P < 0.0001). For both NOCM and NOEXT the A2 mating type isolate with the highest GCA value was Pib-750. Among the A1 mating type isolates, highest GCA values were estimated for Pib-754 and Pib-742, based on the NOCM and NOEXT, respectively. Based on SCA, the best isolate combination for oospores production was between Pib-731 x Pib-755, for both variables. There was no clear trend regarding a possible geographical proximity effect on the fecundity of crosses. Although the crosses produced oospores, viability and germination rates were low, on average 0.3% to 0.03%, respectively. Despite the low viability, production of oospores can be a threat to potato and tomato crops as a source of genetic diversity for this pathogen. |
publishDate |
2006 |
dc.date.available.fl_str_mv |
2006-11-07 2015-03-26T13:37:52Z |
dc.date.issued.fl_str_mv |
2006-08-31 |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:37:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CRUZADO, Zoila Rosa Magnolia Santa Cruz. Viability of oospores originated from crosses of Brazilian isolates of Phytophthora infestans. 2006. 34 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2006. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/4426 |
identifier_str_mv |
CRUZADO, Zoila Rosa Magnolia Santa Cruz. Viability of oospores originated from crosses of Brazilian isolates of Phytophthora infestans. 2006. 34 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2006. |
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http://locus.ufv.br/handle/123456789/4426 |
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por |
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por |
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Universidade Federal de Viçosa |
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Mestrado em Fitopatologia |
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UFV |
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BR |
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Etiologia; Epidemiologia; Controle |
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Universidade Federal de Viçosa |
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