Análise do proteoma do feijoeiro expresso em resposta à antracnose e mancha angular

Detalhes bibliográficos
Autor(a) principal: Borges, Leandro Luiz
Data de Publicação: 2012
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/4772
Resumo: The common bean (Phaseolus vulgaris L.) is a major component of the diet in Brazil and an important primary source of protein and iron. Brazil is the largest common bean producer and the yield of this crop in the country could be higher if it were not, among other reasons, for the numerous diseases affecting this crop. Among the fungal diseases affecting the aerial portion of the plant are the angular leaf spot, caused by Pseudocercospora griseola, and anthracnose, caused by Colletotrichum lindemuthianum. These diseases can cause losses of more than 70% depending on weather conditions, degree of resistance of the cultivar and pathogenicity of the fungal isolates. Understanding how plants respond to pathogen attack is of fundamental importance for the development of resistant cultivars that can ensure the sustainability of agricultural production. This work aimed to identify proteins differentially expressed by the common bean genotype AND 277 in response to P. griseola and C. lindemuthianum. Two independent experiments were conducted. In the first one, leaf samples were collected and evaluated 12, 24 and 48 hours after inoculation (h.a.i.) with P. griseola race 31.31. In the second one, leaf samples were collected and evaluated 12 and 48 h.a.i. of C. lindemuthianum race 73 of. Protein samples were extracted by the phenol-SDS method and separated by two-dimensional electrophoresis. Proteins differentially expressed between inoculated and non-inoculated plants were excised from the gels, cleaved with trypsin and analyzed by mass spectrometry. In the first experiment, 13 differentially expressed proteins were identified, five were detected only in inoculated plants, three showed increased expression and five had reduced expression upon inoculation. These proteins are associated with the defense process, hormone synthesis, photosynthesis, metabolism of reactive oxygen species, cellular respiration and porphyrin synthesis. In the second experiment, 35 differentially expressed proteins were identified, 13 of which were present only in inoculated plants and six only in non- inoculated plants, 10 had increased expression and six were reduced upon inoculation. These proteins participate in cellular detoxification processes, defense against pathogens, metabolism of reactive oxygen species, photosynthesis, cellular respiration, protein biosynthesis and signaling. These results contribute for a better understanding of the defense response of common bean to these pathogens. The identified proteins and their corresponding genes (candidate genes) can be used as tools breeding programs to develop resistant plants.
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spelling Borges, Leandro Luizhttp://lattes.cnpq.br/8212175218924215Ramos, Humberto Josué de Oliveirahttp://lattes.cnpq.br/4037452920080174Oliveira, Leandro Licursi dehttp://lattes.cnpq.br/0578231392218162Barros, Everaldo Gonçalves dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781285J6Arruda, Klever Márcio Antuneshttp://lattes.cnpq.br/48340369007882952015-03-26T13:42:26Z2013-03-272015-03-26T13:42:26Z2012-07-24BORGES, Leandro Luiz. Analysis of the common bean proteome expressed in response to anthracnose and angular leaf spot. 2012. 107 f. Dissertação (Mestrado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2012.http://locus.ufv.br/handle/123456789/4772The common bean (Phaseolus vulgaris L.) is a major component of the diet in Brazil and an important primary source of protein and iron. Brazil is the largest common bean producer and the yield of this crop in the country could be higher if it were not, among other reasons, for the numerous diseases affecting this crop. Among the fungal diseases affecting the aerial portion of the plant are the angular leaf spot, caused by Pseudocercospora griseola, and anthracnose, caused by Colletotrichum lindemuthianum. These diseases can cause losses of more than 70% depending on weather conditions, degree of resistance of the cultivar and pathogenicity of the fungal isolates. Understanding how plants respond to pathogen attack is of fundamental importance for the development of resistant cultivars that can ensure the sustainability of agricultural production. This work aimed to identify proteins differentially expressed by the common bean genotype AND 277 in response to P. griseola and C. lindemuthianum. Two independent experiments were conducted. In the first one, leaf samples were collected and evaluated 12, 24 and 48 hours after inoculation (h.a.i.) with P. griseola race 31.31. In the second one, leaf samples were collected and evaluated 12 and 48 h.a.i. of C. lindemuthianum race 73 of. Protein samples were extracted by the phenol-SDS method and separated by two-dimensional electrophoresis. Proteins differentially expressed between inoculated and non-inoculated plants were excised from the gels, cleaved with trypsin and analyzed by mass spectrometry. In the first experiment, 13 differentially expressed proteins were identified, five were detected only in inoculated plants, three showed increased expression and five had reduced expression upon inoculation. These proteins are associated with the defense process, hormone synthesis, photosynthesis, metabolism of reactive oxygen species, cellular respiration and porphyrin synthesis. In the second experiment, 35 differentially expressed proteins were identified, 13 of which were present only in inoculated plants and six only in non- inoculated plants, 10 had increased expression and six were reduced upon inoculation. These proteins participate in cellular detoxification processes, defense against pathogens, metabolism of reactive oxygen species, photosynthesis, cellular respiration, protein biosynthesis and signaling. These results contribute for a better understanding of the defense response of common bean to these pathogens. The identified proteins and their corresponding genes (candidate genes) can be used as tools breeding programs to develop resistant plants.O feijoeiro comum (Phaseolus vulgaris L.) é um dos principais componentes da dieta do brasileiro além de ser uma importante fonte primária de proteína e ferro. O Brasil é o maior produtor dessa leguminosa. Um dos fatores que limitam a produtividade brasileira é o grande número de doenças. Dentre as doenças fúngicas da parte aérea destacam-se a mancha angular causada por Pseudocercospora griseola e a antracnose, causada por Colletotrichum lindemuthianum. Essas doenças podem causar perdas superiores a 70% dependendo das condições climáticas, suscetibilidade da cultivar e patogenicidade dos isolados. A compreensão de como as plantas respondem ao ataque de patógenos é de fundamental importância para o desenvolvimento de cultivares resistentes que possam garantir a sustentabilidade da produção agrícola. Com este trabalho objetivou-se identificar proteínas diferencialmente expressas pelo genótipo de feijoeiro comum AND 277 em resposta a P. griseola e C. lindemuthianum. Foram realizados dois experimentos independentes. No primeiro, foram avaliadas amostras de folhas coletadas 12, 24 e 48 horas após a inoculação (h.a.i.) da raça 31.31 de P. griseola. No segundo, foram avaliadas amostras de folhas coletadas 12 e 48 h.a.i. da raça 73 de C. lindemuthianum. Amostras de proteínas foram extraídas pelo método fenol-SDS e separadas por eletroforese bidimensional. Proteínas diferencialmente expressas entre plantas inoculadas e não inoculadas com o patógeno foram excisadas dos géis, clivadas com tripsina e analisadas por espectrometria de massa. No primeiro experimento, foram identificadas 13 proteínas diferencialmente expressas, sendo que cinco foram detectadas somente nas plantas inoculadas, três apresentaram aumento de expressão e cinco tiveram redução da expressão após inoculação. Essas proteínas estão associadas a processos de defesa, síntese de hormônios, fotossíntese, metabolismo de espécies reativas de oxigênio, respiração celular e síntese de porfirinas. No segundo experimento, foram identificadas 35 proteínas diferencialmente expressas, sendo que 13 estavam presentes somente nas plantas inoculadas e seis somente nas plantas não inoculadas, 10 proteínas tiveram aumento de expressão e seis tiveram redução após a inoculação. Essas proteínas participam de processos de detoxificação celular, defesa contra patógenos, metabolismo de espécies reativas de oxigênio, fotossíntese, respiração celular, biossíntese de proteínas e sinalização. Esses resultados contribuem para um melhor entendimento do mecanismo de resposta de defesa do feijoeiro comum a esses patógenos. As proteínas identificadas e os genes que as codificam (genes candidatos) poderão auxiliar como ferramentas em programas de melhoramento genético no desenvolvimento de plantas resistentes.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em Genética e MelhoramentoUFVBRGenética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; MeProteômicaPhaseolus vulgarisPseudocercospora griseolaColletotrichum lindemuthianumProteomicsPhaseolus vulgarisPseudocercospora griseolaColletotrichum lindemuthianumCNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA VEGETALAnálise do proteoma do feijoeiro expresso em resposta à antracnose e mancha angularAnalysis of the common bean proteome expressed in response to anthracnose and angular leaf spotinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf1757825https://locus.ufv.br//bitstream/123456789/4772/1/texto%20completo.pdf4e0395e0f72e73520cb71461e4c59bbeMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain200155https://locus.ufv.br//bitstream/123456789/4772/2/texto%20completo.pdf.txt5dfa5fbabca84f5197adf698c313e484MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3632https://locus.ufv.br//bitstream/123456789/4772/3/texto%20completo.pdf.jpg161d1d53d5b1023268f147e3f924b8b4MD53123456789/47722016-04-10 23:03:45.315oai:locus.ufv.br:123456789/4772Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:03:45LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Análise do proteoma do feijoeiro expresso em resposta à antracnose e mancha angular
dc.title.alternative.eng.fl_str_mv Analysis of the common bean proteome expressed in response to anthracnose and angular leaf spot
title Análise do proteoma do feijoeiro expresso em resposta à antracnose e mancha angular
spellingShingle Análise do proteoma do feijoeiro expresso em resposta à antracnose e mancha angular
Borges, Leandro Luiz
Proteômica
Phaseolus vulgaris
Pseudocercospora griseola
Colletotrichum lindemuthianum
Proteomics
Phaseolus vulgaris
Pseudocercospora griseola
Colletotrichum lindemuthianum
CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA VEGETAL
title_short Análise do proteoma do feijoeiro expresso em resposta à antracnose e mancha angular
title_full Análise do proteoma do feijoeiro expresso em resposta à antracnose e mancha angular
title_fullStr Análise do proteoma do feijoeiro expresso em resposta à antracnose e mancha angular
title_full_unstemmed Análise do proteoma do feijoeiro expresso em resposta à antracnose e mancha angular
title_sort Análise do proteoma do feijoeiro expresso em resposta à antracnose e mancha angular
author Borges, Leandro Luiz
author_facet Borges, Leandro Luiz
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/8212175218924215
dc.contributor.author.fl_str_mv Borges, Leandro Luiz
dc.contributor.advisor-co1.fl_str_mv Ramos, Humberto Josué de Oliveira
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/4037452920080174
dc.contributor.advisor-co2.fl_str_mv Oliveira, Leandro Licursi de
dc.contributor.advisor-co2Lattes.fl_str_mv http://lattes.cnpq.br/0578231392218162
dc.contributor.advisor1.fl_str_mv Barros, Everaldo Gonçalves de
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781285J6
dc.contributor.referee1.fl_str_mv Arruda, Klever Márcio Antunes
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/4834036900788295
contributor_str_mv Ramos, Humberto Josué de Oliveira
Oliveira, Leandro Licursi de
Barros, Everaldo Gonçalves de
Arruda, Klever Márcio Antunes
dc.subject.por.fl_str_mv Proteômica
Phaseolus vulgaris
Pseudocercospora griseola
Colletotrichum lindemuthianum
topic Proteômica
Phaseolus vulgaris
Pseudocercospora griseola
Colletotrichum lindemuthianum
Proteomics
Phaseolus vulgaris
Pseudocercospora griseola
Colletotrichum lindemuthianum
CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA VEGETAL
dc.subject.eng.fl_str_mv Proteomics
Phaseolus vulgaris
Pseudocercospora griseola
Colletotrichum lindemuthianum
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA VEGETAL
description The common bean (Phaseolus vulgaris L.) is a major component of the diet in Brazil and an important primary source of protein and iron. Brazil is the largest common bean producer and the yield of this crop in the country could be higher if it were not, among other reasons, for the numerous diseases affecting this crop. Among the fungal diseases affecting the aerial portion of the plant are the angular leaf spot, caused by Pseudocercospora griseola, and anthracnose, caused by Colletotrichum lindemuthianum. These diseases can cause losses of more than 70% depending on weather conditions, degree of resistance of the cultivar and pathogenicity of the fungal isolates. Understanding how plants respond to pathogen attack is of fundamental importance for the development of resistant cultivars that can ensure the sustainability of agricultural production. This work aimed to identify proteins differentially expressed by the common bean genotype AND 277 in response to P. griseola and C. lindemuthianum. Two independent experiments were conducted. In the first one, leaf samples were collected and evaluated 12, 24 and 48 hours after inoculation (h.a.i.) with P. griseola race 31.31. In the second one, leaf samples were collected and evaluated 12 and 48 h.a.i. of C. lindemuthianum race 73 of. Protein samples were extracted by the phenol-SDS method and separated by two-dimensional electrophoresis. Proteins differentially expressed between inoculated and non-inoculated plants were excised from the gels, cleaved with trypsin and analyzed by mass spectrometry. In the first experiment, 13 differentially expressed proteins were identified, five were detected only in inoculated plants, three showed increased expression and five had reduced expression upon inoculation. These proteins are associated with the defense process, hormone synthesis, photosynthesis, metabolism of reactive oxygen species, cellular respiration and porphyrin synthesis. In the second experiment, 35 differentially expressed proteins were identified, 13 of which were present only in inoculated plants and six only in non- inoculated plants, 10 had increased expression and six were reduced upon inoculation. These proteins participate in cellular detoxification processes, defense against pathogens, metabolism of reactive oxygen species, photosynthesis, cellular respiration, protein biosynthesis and signaling. These results contribute for a better understanding of the defense response of common bean to these pathogens. The identified proteins and their corresponding genes (candidate genes) can be used as tools breeding programs to develop resistant plants.
publishDate 2012
dc.date.issued.fl_str_mv 2012-07-24
dc.date.available.fl_str_mv 2013-03-27
2015-03-26T13:42:26Z
dc.date.accessioned.fl_str_mv 2015-03-26T13:42:26Z
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dc.identifier.citation.fl_str_mv BORGES, Leandro Luiz. Analysis of the common bean proteome expressed in response to anthracnose and angular leaf spot. 2012. 107 f. Dissertação (Mestrado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2012.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/4772
identifier_str_mv BORGES, Leandro Luiz. Analysis of the common bean proteome expressed in response to anthracnose and angular leaf spot. 2012. 107 f. Dissertação (Mestrado em Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me) - Universidade Federal de Viçosa, Viçosa, 2012.
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dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me
publisher.none.fl_str_mv Universidade Federal de Viçosa
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