Atividade antiviral de extratos vegetais e flavonoides contra o Bovine herpesvirus 1 (BoHV1)

• Autor(a) principal: Tafuri, Natalia Filardi
• Data de Publicação: 2011
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2433
• topic: Flavonoides; Bovine herpesvirus 1; Extratos vegetais; Flavonoids; Bovine herpesvirus 1; Plants extracts; CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR

The infections turn are had still as challenge for the public health and agricultural section. Several studies have as focus the discovery of new antiviral derived of natural products, mainly plants. The herpesvirus are one of the most used groups on this kind of research, due to importance for the public health and economical impact. Therefore, the purpose of this work was to evaluate the antiviral effects of flavonoids and three plants extract on the in vitro replication of bovine herpesvirus 1 (BoHV1). The the maximum non-cytotoxic concentration (CMNT) of DMSO and compounds it was measured by a variation of the method colorimeter based on the reduction of the salt MTT. The results showed that CMNT was 20 μg/mL for quercetina, 1.5 μg/mL for μg/mL and 1.5% for DMSO. For hexanic, dichloromethane, and etanolic extracts the values were 40, 25, and 60 μg/mL, respectively. Compounds' antiviral activity was evaluated through the rehearsal of determination of the virucidal activity and rehearsal of time of addition of the drugs in different stages of the cycle replicativo viral (timing of addition assays). DMSO did not present any activity in none of the accomplished experimental tests. All of substances presented significant virucidal effect. The quercetin and the dichloromethane extract demonstrated effect in the rehearsal of addition of the drug in the stages of adsorption andpenetration viral. The hexanic extract showed reduction of the title viral just in the phase of penetration stage. The ethanolic extract interfered on all stages of the replication in vitro of BoHV1, showing to be the most effective compound. The results of this work suggest the importance of the evaluated compounds for the veterinary and human medicine since they appear to be promising for the development of new antiviral therapies.