Atividade antiviral de extratos vegetais e flavonoides contra o Bovine herpesvirus 1 (BoHV1)
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/2433 |
Resumo: | The infections turn are had still as challenge for the public health and agricultural section. Several studies have as focus the discovery of new antiviral derived of natural products, mainly plants. The herpesvirus are one of the most used groups on this kind of research, due to importance for the public health and economical impact. Therefore, the purpose of this work was to evaluate the antiviral effects of flavonoids and three plants extract on the in vitro replication of bovine herpesvirus 1 (BoHV1). The the maximum non-cytotoxic concentration (CMNT) of DMSO and compounds it was measured by a variation of the method colorimeter based on the reduction of the salt MTT. The results showed that CMNT was 20 μg/mL for quercetina, 1.5 μg/mL for μg/mL and 1.5% for DMSO. For hexanic, dichloromethane, and etanolic extracts the values were 40, 25, and 60 μg/mL, respectively. Compounds' antiviral activity was evaluated through the rehearsal of determination of the virucidal activity and rehearsal of time of addition of the drugs in different stages of the cycle replicativo viral (timing of addition assays). DMSO did not present any activity in none of the accomplished experimental tests. All of substances presented significant virucidal effect. The quercetin and the dichloromethane extract demonstrated effect in the rehearsal of addition of the drug in the stages of adsorption andpenetration viral. The hexanic extract showed reduction of the title viral just in the phase of penetration stage. The ethanolic extract interfered on all stages of the replication in vitro of BoHV1, showing to be the most effective compound. The results of this work suggest the importance of the evaluated compounds for the veterinary and human medicine since they appear to be promising for the development of new antiviral therapies. |
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Tafuri, Natalia Filardihttp://lattes.cnpq.br/5064811918480456Silva Júnior, Abelardohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4771645P8Diaz, Marisa Alves Nogueirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723344J7Lamêgo, Márcia Rogéria de Almeidahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782197P4Peternelli, Ethel Fernandes de Oliveirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4704048Z8Santana, Luiz Albertohttp://lattes.cnpq.br/00189608102771482015-03-26T13:07:32Z2012-04-162015-03-26T13:07:32Z2011-02-22TAFURI, Natalia Filardi. Antiviral activity of plants extracts and flavonoids against Bovine herpesvirus 1 (BoHV1). 2011. 85 f. Dissertação (Mestrado em Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal) - Universidade Federal de Viçosa, Viçosa, 2011.http://locus.ufv.br/handle/123456789/2433The infections turn are had still as challenge for the public health and agricultural section. Several studies have as focus the discovery of new antiviral derived of natural products, mainly plants. The herpesvirus are one of the most used groups on this kind of research, due to importance for the public health and economical impact. Therefore, the purpose of this work was to evaluate the antiviral effects of flavonoids and three plants extract on the in vitro replication of bovine herpesvirus 1 (BoHV1). The the maximum non-cytotoxic concentration (CMNT) of DMSO and compounds it was measured by a variation of the method colorimeter based on the reduction of the salt MTT. The results showed that CMNT was 20 μg/mL for quercetina, 1.5 μg/mL for μg/mL and 1.5% for DMSO. For hexanic, dichloromethane, and etanolic extracts the values were 40, 25, and 60 μg/mL, respectively. Compounds' antiviral activity was evaluated through the rehearsal of determination of the virucidal activity and rehearsal of time of addition of the drugs in different stages of the cycle replicativo viral (timing of addition assays). DMSO did not present any activity in none of the accomplished experimental tests. All of substances presented significant virucidal effect. The quercetin and the dichloromethane extract demonstrated effect in the rehearsal of addition of the drug in the stages of adsorption andpenetration viral. The hexanic extract showed reduction of the title viral just in the phase of penetration stage. The ethanolic extract interfered on all stages of the replication in vitro of BoHV1, showing to be the most effective compound. The results of this work suggest the importance of the evaluated compounds for the veterinary and human medicine since they appear to be promising for the development of new antiviral therapies.As infecções virais ainda são tidas como desafio para a saúde pública e setor agropecuário. Diversos estudos têm como foco a descoberta de novos antivirais derivados de produtos naturais, principalmente vegetais. Os herpesvírus são uma classe muito utilizada nestas pesquisas, devido à importância para a saúde pública e impacto econômico. Neste contexto, o presente estudo busca avaliar o efeito in vitro de dois flavonoides e três extratos vegetais, na replicação in vitro do bovine herpesvirus 1 (BoHV1). A concentração máxima não tóxica (CMNT) do DMSO e dos compostos foi mensurada por uma variação do método colorimétrico baseado na redução do sal MTT. As CMNT obtidas foram 20μg/mL para quercetina, 1,5μg/mL para o TMDMB2, 1,5% (v/v) para o DMSO e 40, 25 e 60 μg/mL para os extratos hexânico, diclorometano e etanólico respectivamente. A atividade antiviral dos compostos foi avaliada por meio do ensaio de determinação da atividade virucida e ensaio de tempo de adição das drogas em diferentes etapas do ciclo replicativo viral (ensaio timing of addition). O DMSO não apresentou atividade antiviral em nenhum dos ensaios realizados. Todas as substâncias avaliadas apresentaram significante efeito virucida. A quercetina e o extrato diclorometano demonstraram efeito no ensaio de adição da droga nas etapas de adsorção e penetração viral. O extrato hexânico, mostrou redução do título viral apenas na fase de pentraçao viral. O extrato etanólico interferiu em todas as etapas da replicação in vitro do BoHV1, se mostrando o composto mais eficaz. Os resultados deste trabalho sugerem a importância dos compostos avaliados para a medicina veterinária e humana, pois eles se mostraram promissores para o desenvolvimento de novas terapias antivirais.Fundação de Amparo a Pesquisa do Estado de Minas Geraisapplication/pdfporUniversidade Federal de ViçosaMestrado em Bioquímica AgrícolaUFVBRBioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animalFlavonoidesBovine herpesvirus 1Extratos vegetaisFlavonoidsBovine herpesvirus 1Plants extractsCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULARAtividade antiviral de extratos vegetais e flavonoides contra o Bovine herpesvirus 1 (BoHV1)Antiviral activity of plants extracts and flavonoids against Bovine herpesvirus 1 (BoHV1)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf1702771https://locus.ufv.br//bitstream/123456789/2433/1/texto%20completo.pdf1786167a286f34b12223afcf6f567864MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain157511https://locus.ufv.br//bitstream/123456789/2433/2/texto%20completo.pdf.txt9e57fc1eabe6610c3c709844bf243bd3MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3637https://locus.ufv.br//bitstream/123456789/2433/3/texto%20completo.pdf.jpg37daf17a15a6a6694083477d6041ab7dMD53123456789/24332017-04-19 14:06:57.624oai:locus.ufv.br:123456789/2433Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-04-19T17:06:57LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Atividade antiviral de extratos vegetais e flavonoides contra o Bovine herpesvirus 1 (BoHV1) |
dc.title.alternative.eng.fl_str_mv |
Antiviral activity of plants extracts and flavonoids against Bovine herpesvirus 1 (BoHV1) |
title |
Atividade antiviral de extratos vegetais e flavonoides contra o Bovine herpesvirus 1 (BoHV1) |
spellingShingle |
Atividade antiviral de extratos vegetais e flavonoides contra o Bovine herpesvirus 1 (BoHV1) Tafuri, Natalia Filardi Flavonoides Bovine herpesvirus 1 Extratos vegetais Flavonoids Bovine herpesvirus 1 Plants extracts CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
title_short |
Atividade antiviral de extratos vegetais e flavonoides contra o Bovine herpesvirus 1 (BoHV1) |
title_full |
Atividade antiviral de extratos vegetais e flavonoides contra o Bovine herpesvirus 1 (BoHV1) |
title_fullStr |
Atividade antiviral de extratos vegetais e flavonoides contra o Bovine herpesvirus 1 (BoHV1) |
title_full_unstemmed |
Atividade antiviral de extratos vegetais e flavonoides contra o Bovine herpesvirus 1 (BoHV1) |
title_sort |
Atividade antiviral de extratos vegetais e flavonoides contra o Bovine herpesvirus 1 (BoHV1) |
author |
Tafuri, Natalia Filardi |
author_facet |
Tafuri, Natalia Filardi |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/5064811918480456 |
dc.contributor.author.fl_str_mv |
Tafuri, Natalia Filardi |
dc.contributor.advisor-co1.fl_str_mv |
Silva Júnior, Abelardo |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4771645P8 |
dc.contributor.advisor-co2.fl_str_mv |
Diaz, Marisa Alves Nogueira |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723344J7 |
dc.contributor.advisor1.fl_str_mv |
Lamêgo, Márcia Rogéria de Almeida |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782197P4 |
dc.contributor.referee1.fl_str_mv |
Peternelli, Ethel Fernandes de Oliveira |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4704048Z8 |
dc.contributor.referee2.fl_str_mv |
Santana, Luiz Alberto |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/0018960810277148 |
contributor_str_mv |
Silva Júnior, Abelardo Diaz, Marisa Alves Nogueira Lamêgo, Márcia Rogéria de Almeida Peternelli, Ethel Fernandes de Oliveira Santana, Luiz Alberto |
dc.subject.por.fl_str_mv |
Flavonoides Bovine herpesvirus 1 Extratos vegetais |
topic |
Flavonoides Bovine herpesvirus 1 Extratos vegetais Flavonoids Bovine herpesvirus 1 Plants extracts CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
dc.subject.eng.fl_str_mv |
Flavonoids Bovine herpesvirus 1 Plants extracts |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
description |
The infections turn are had still as challenge for the public health and agricultural section. Several studies have as focus the discovery of new antiviral derived of natural products, mainly plants. The herpesvirus are one of the most used groups on this kind of research, due to importance for the public health and economical impact. Therefore, the purpose of this work was to evaluate the antiviral effects of flavonoids and three plants extract on the in vitro replication of bovine herpesvirus 1 (BoHV1). The the maximum non-cytotoxic concentration (CMNT) of DMSO and compounds it was measured by a variation of the method colorimeter based on the reduction of the salt MTT. The results showed that CMNT was 20 μg/mL for quercetina, 1.5 μg/mL for μg/mL and 1.5% for DMSO. For hexanic, dichloromethane, and etanolic extracts the values were 40, 25, and 60 μg/mL, respectively. Compounds' antiviral activity was evaluated through the rehearsal of determination of the virucidal activity and rehearsal of time of addition of the drugs in different stages of the cycle replicativo viral (timing of addition assays). DMSO did not present any activity in none of the accomplished experimental tests. All of substances presented significant virucidal effect. The quercetin and the dichloromethane extract demonstrated effect in the rehearsal of addition of the drug in the stages of adsorption andpenetration viral. The hexanic extract showed reduction of the title viral just in the phase of penetration stage. The ethanolic extract interfered on all stages of the replication in vitro of BoHV1, showing to be the most effective compound. The results of this work suggest the importance of the evaluated compounds for the veterinary and human medicine since they appear to be promising for the development of new antiviral therapies. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-02-22 |
dc.date.available.fl_str_mv |
2012-04-16 2015-03-26T13:07:32Z |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:07:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
TAFURI, Natalia Filardi. Antiviral activity of plants extracts and flavonoids against Bovine herpesvirus 1 (BoHV1). 2011. 85 f. Dissertação (Mestrado em Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal) - Universidade Federal de Viçosa, Viçosa, 2011. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/2433 |
identifier_str_mv |
TAFURI, Natalia Filardi. Antiviral activity of plants extracts and flavonoids against Bovine herpesvirus 1 (BoHV1). 2011. 85 f. Dissertação (Mestrado em Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal) - Universidade Federal de Viçosa, Viçosa, 2011. |
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http://locus.ufv.br/handle/123456789/2433 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de Viçosa |
dc.publisher.program.fl_str_mv |
Mestrado em Bioquímica Agrícola |
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UFV |
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BR |
dc.publisher.department.fl_str_mv |
Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal |
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Universidade Federal de Viçosa |
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