Avaliação dos peptídeos sintéticos SBbo23290 e SBm7462 na forma monovalente e polivalente em bovinos desafiados com Riphicephalus (Boophilus) microplus (CANESTRINI, 1887) e Babesia bovis (BABES, 1888; STARCOVICI, 1888)
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/5079 |
Resumo: | It was evaluated the immunology response given by the SBbo23290 and SBm7462 synthetic peptides to the control of Babesia bovis and Riphicephalus (Boophilus) microplus, inoculated by subcutaneous route, individually and simultaneously (Group I) and in a cocktail of both peptides (Group II). Each inoculation was done with intervals of 30 days. In the 30th and 34th days after the 3th inoculation, it was applied in each animal, 4000 larvaes of R. (B.) microplus, free of blood parasites and also, it was inoculated a sample of Babesia bovis UFV1 9th passage with a concentration of 1x106 babesias/mL, respectively. According to the results, the animals inoculated with the Group II obtained greater clinic response after challenging with both parasites. Besides, these animals showed high levels of total IgG specific to the SBbo23290, beginning one week after 1st inoculation, with titles of 1,06 ± 0,2486. The titles with high affinity of total IgG to SBm7462 was presented from the 8th week, with levels of 1,75 ± 0,1849 and 1,13 ± 0,2819 to Groups I and II, respectively. Both Group showed high levels of IgG1 over IgG2 isotopes with high affinity to SBbo23290. The Group I showed titles of total IgG and IgG1 with high affinity to SBm7462 and low efficiency (EF) to control the ticks, different of the obtained in the Group II, where it was found high titles of total IgG, low levels of IgG1 and EF of 46,80 %. These results suggest that, intraclonal competition was present after presentation by APCs (DC or WC1.1+) to CD4+ and lymphocytes B in the germinal centers, with major affinity by specific clones to SBbo23290 over SBm7462. It is possible that these changes were determined by antigen solubility. |
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Montano, Javier Antonio Benavideshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4109604E5Viloria, Marlene Isabel Vargashttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781964E6Araújo, Jackson Victor dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780836D6Salcedo, Joaquín Hernán Patarroyohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783313T4Moreira, Maria Aparecida Scatamburlohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4797678J6Paula, Sérgio Oliveira dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767540P42015-03-26T13:47:01Z2007-02-072015-03-26T13:47:01Z2006-12-14MONTANO, Javier Antonio Benavides. Evaluation of synthetic peptides SBbo23290 and SBm7462 at monovalent and polyvalent presentations in challenged cattle with Riphicephalus (Boophilus) microplus (CANESTRINI, 1887) and Babesia bovis (BABES, 1888; STARCOVICI, 1888). 2006. 72 f. Dissertação (Mestrado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2006.http://locus.ufv.br/handle/123456789/5079It was evaluated the immunology response given by the SBbo23290 and SBm7462 synthetic peptides to the control of Babesia bovis and Riphicephalus (Boophilus) microplus, inoculated by subcutaneous route, individually and simultaneously (Group I) and in a cocktail of both peptides (Group II). Each inoculation was done with intervals of 30 days. In the 30th and 34th days after the 3th inoculation, it was applied in each animal, 4000 larvaes of R. (B.) microplus, free of blood parasites and also, it was inoculated a sample of Babesia bovis UFV1 9th passage with a concentration of 1x106 babesias/mL, respectively. According to the results, the animals inoculated with the Group II obtained greater clinic response after challenging with both parasites. Besides, these animals showed high levels of total IgG specific to the SBbo23290, beginning one week after 1st inoculation, with titles of 1,06 ± 0,2486. The titles with high affinity of total IgG to SBm7462 was presented from the 8th week, with levels of 1,75 ± 0,1849 and 1,13 ± 0,2819 to Groups I and II, respectively. Both Group showed high levels of IgG1 over IgG2 isotopes with high affinity to SBbo23290. The Group I showed titles of total IgG and IgG1 with high affinity to SBm7462 and low efficiency (EF) to control the ticks, different of the obtained in the Group II, where it was found high titles of total IgG, low levels of IgG1 and EF of 46,80 %. These results suggest that, intraclonal competition was present after presentation by APCs (DC or WC1.1+) to CD4+ and lymphocytes B in the germinal centers, with major affinity by specific clones to SBbo23290 over SBm7462. It is possible that these changes were determined by antigen solubility.Avaliou-se a resposta imunológica conferida pelos peptídeos SBbo23290 e SBm7462 para o controle de Babesia bovis e Riphicephalus (Boophilus) microplus, ao serem aplicados simultaneamente na apresentação individual (Group I) e em associação (Group II). Foram realizadas três aplicações em cada grupo, via subcutânea, com intervalos de 30 dias. O desafio foi feito 30º e 34º dias após a 3ª inoculação, colocando-se 4000 larvas de R. (B.) microplus, cepa livre de hemoparasitas, e inoculando-se a cepa de B. bovis UFV1 9ª passagem numa concentração de 1x106 parasitas/mL, respectivamente. De acordo com os resultados obtidos, os animais do Grupo II, apresentaram uma melhor resposta clínica frente ao desafio, acompanhado de altos níveis de IgG total específicos para o peptídeo SBbo23290, a partir da 1ª semana após a inoculação, com títulos de 1,06 ± 0,2486. Os títulos de IgG total específicos para SBm7462 estiveram presentes a partir da 8ª semana com níveis de 1,75 ± 0,1849 e 1,13 ± 0,2819 para Grupo I e II, respectivamente. Ambos os tratamentos apresentaram maiores níveis de isotipos IgG1 sobre IgG2 específicos para SBbo23290. O Grupo I, apresentou títulos de IgG total e IgG1 específicos para SBm7462 e baixa eficácia (EF) no controle dos carrapatos, diferente do obtido no Grupo II, onde encontrou-se altos títulos de IgG total, níveis baixos do IgG1 e uma EF de 46,80 %. Sugere-se que estes resultados foram determinados por competição intraclonal em populações após a apresentação por APCs (DC ou WC1.1+) a CD4+ e linfócitos B nos centros germinais, com maior afinidade por clones específicos para SBbo23290 sobre SBm7462, talvez determinado por solubilidade antigênica.Fundação de Amparo a Pesquisa do Estado de Minas Geraisapplication/pdfporUniversidade Federal de ViçosaMestrado em Medicina VeterináriaUFVBRBiotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. deBabesia bovisRiphicephalus boophilusBoophilus microplusResposta imune-humoralBabesia bovisRiphicephalus boophilusBoophilus microplusImmunology responseCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::MEDICINA VETERINARIA PREVENTIVAAvaliação dos peptídeos sintéticos SBbo23290 e SBm7462 na forma monovalente e polivalente em bovinos desafiados com Riphicephalus (Boophilus) microplus (CANESTRINI, 1887) e Babesia bovis (BABES, 1888; STARCOVICI, 1888)Evaluation of synthetic peptides SBbo23290 and SBm7462 at monovalent and polyvalent presentations in challenged cattle with Riphicephalus (Boophilus) microplus (CANESTRINI, 1887) and Babesia bovis (BABES, 1888; STARCOVICI, 1888)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf424006https://locus.ufv.br//bitstream/123456789/5079/1/texto%20completo.pdfe70b787e481758ae2e9bc9b63c6cb946MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain125325https://locus.ufv.br//bitstream/123456789/5079/2/texto%20completo.pdf.txt1b27862975ecad0bc9ba02ccfe29eb4fMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3786https://locus.ufv.br//bitstream/123456789/5079/3/texto%20completo.pdf.jpg2315781c0ccd7b22093bdaa6e4744c6aMD53123456789/50792016-04-11 23:07:21.795oai:locus.ufv.br:123456789/5079Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-12T02:07:21LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Avaliação dos peptídeos sintéticos SBbo23290 e SBm7462 na forma monovalente e polivalente em bovinos desafiados com Riphicephalus (Boophilus) microplus (CANESTRINI, 1887) e Babesia bovis (BABES, 1888; STARCOVICI, 1888) |
dc.title.alternative.eng.fl_str_mv |
Evaluation of synthetic peptides SBbo23290 and SBm7462 at monovalent and polyvalent presentations in challenged cattle with Riphicephalus (Boophilus) microplus (CANESTRINI, 1887) and Babesia bovis (BABES, 1888; STARCOVICI, 1888) |
title |
Avaliação dos peptídeos sintéticos SBbo23290 e SBm7462 na forma monovalente e polivalente em bovinos desafiados com Riphicephalus (Boophilus) microplus (CANESTRINI, 1887) e Babesia bovis (BABES, 1888; STARCOVICI, 1888) |
spellingShingle |
Avaliação dos peptídeos sintéticos SBbo23290 e SBm7462 na forma monovalente e polivalente em bovinos desafiados com Riphicephalus (Boophilus) microplus (CANESTRINI, 1887) e Babesia bovis (BABES, 1888; STARCOVICI, 1888) Montano, Javier Antonio Benavides Babesia bovis Riphicephalus boophilus Boophilus microplus Resposta imune-humoral Babesia bovis Riphicephalus boophilus Boophilus microplus Immunology response CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::MEDICINA VETERINARIA PREVENTIVA |
title_short |
Avaliação dos peptídeos sintéticos SBbo23290 e SBm7462 na forma monovalente e polivalente em bovinos desafiados com Riphicephalus (Boophilus) microplus (CANESTRINI, 1887) e Babesia bovis (BABES, 1888; STARCOVICI, 1888) |
title_full |
Avaliação dos peptídeos sintéticos SBbo23290 e SBm7462 na forma monovalente e polivalente em bovinos desafiados com Riphicephalus (Boophilus) microplus (CANESTRINI, 1887) e Babesia bovis (BABES, 1888; STARCOVICI, 1888) |
title_fullStr |
Avaliação dos peptídeos sintéticos SBbo23290 e SBm7462 na forma monovalente e polivalente em bovinos desafiados com Riphicephalus (Boophilus) microplus (CANESTRINI, 1887) e Babesia bovis (BABES, 1888; STARCOVICI, 1888) |
title_full_unstemmed |
Avaliação dos peptídeos sintéticos SBbo23290 e SBm7462 na forma monovalente e polivalente em bovinos desafiados com Riphicephalus (Boophilus) microplus (CANESTRINI, 1887) e Babesia bovis (BABES, 1888; STARCOVICI, 1888) |
title_sort |
Avaliação dos peptídeos sintéticos SBbo23290 e SBm7462 na forma monovalente e polivalente em bovinos desafiados com Riphicephalus (Boophilus) microplus (CANESTRINI, 1887) e Babesia bovis (BABES, 1888; STARCOVICI, 1888) |
author |
Montano, Javier Antonio Benavides |
author_facet |
Montano, Javier Antonio Benavides |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4109604E5 |
dc.contributor.author.fl_str_mv |
Montano, Javier Antonio Benavides |
dc.contributor.advisor-co1.fl_str_mv |
Viloria, Marlene Isabel Vargas |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781964E6 |
dc.contributor.advisor-co2.fl_str_mv |
Araújo, Jackson Victor de |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780836D6 |
dc.contributor.advisor1.fl_str_mv |
Salcedo, Joaquín Hernán Patarroyo |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783313T4 |
dc.contributor.referee1.fl_str_mv |
Moreira, Maria Aparecida Scatamburlo |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4797678J6 |
dc.contributor.referee2.fl_str_mv |
Paula, Sérgio Oliveira de |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767540P4 |
contributor_str_mv |
Viloria, Marlene Isabel Vargas Araújo, Jackson Victor de Salcedo, Joaquín Hernán Patarroyo Moreira, Maria Aparecida Scatamburlo Paula, Sérgio Oliveira de |
dc.subject.por.fl_str_mv |
Babesia bovis Riphicephalus boophilus Boophilus microplus Resposta imune-humoral |
topic |
Babesia bovis Riphicephalus boophilus Boophilus microplus Resposta imune-humoral Babesia bovis Riphicephalus boophilus Boophilus microplus Immunology response CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::MEDICINA VETERINARIA PREVENTIVA |
dc.subject.eng.fl_str_mv |
Babesia bovis Riphicephalus boophilus Boophilus microplus Immunology response |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::MEDICINA VETERINARIA PREVENTIVA |
description |
It was evaluated the immunology response given by the SBbo23290 and SBm7462 synthetic peptides to the control of Babesia bovis and Riphicephalus (Boophilus) microplus, inoculated by subcutaneous route, individually and simultaneously (Group I) and in a cocktail of both peptides (Group II). Each inoculation was done with intervals of 30 days. In the 30th and 34th days after the 3th inoculation, it was applied in each animal, 4000 larvaes of R. (B.) microplus, free of blood parasites and also, it was inoculated a sample of Babesia bovis UFV1 9th passage with a concentration of 1x106 babesias/mL, respectively. According to the results, the animals inoculated with the Group II obtained greater clinic response after challenging with both parasites. Besides, these animals showed high levels of total IgG specific to the SBbo23290, beginning one week after 1st inoculation, with titles of 1,06 ± 0,2486. The titles with high affinity of total IgG to SBm7462 was presented from the 8th week, with levels of 1,75 ± 0,1849 and 1,13 ± 0,2819 to Groups I and II, respectively. Both Group showed high levels of IgG1 over IgG2 isotopes with high affinity to SBbo23290. The Group I showed titles of total IgG and IgG1 with high affinity to SBm7462 and low efficiency (EF) to control the ticks, different of the obtained in the Group II, where it was found high titles of total IgG, low levels of IgG1 and EF of 46,80 %. These results suggest that, intraclonal competition was present after presentation by APCs (DC or WC1.1+) to CD4+ and lymphocytes B in the germinal centers, with major affinity by specific clones to SBbo23290 over SBm7462. It is possible that these changes were determined by antigen solubility. |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006-12-14 |
dc.date.available.fl_str_mv |
2007-02-07 2015-03-26T13:47:01Z |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:47:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MONTANO, Javier Antonio Benavides. Evaluation of synthetic peptides SBbo23290 and SBm7462 at monovalent and polyvalent presentations in challenged cattle with Riphicephalus (Boophilus) microplus (CANESTRINI, 1887) and Babesia bovis (BABES, 1888; STARCOVICI, 1888). 2006. 72 f. Dissertação (Mestrado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2006. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/5079 |
identifier_str_mv |
MONTANO, Javier Antonio Benavides. Evaluation of synthetic peptides SBbo23290 and SBm7462 at monovalent and polyvalent presentations in challenged cattle with Riphicephalus (Boophilus) microplus (CANESTRINI, 1887) and Babesia bovis (BABES, 1888; STARCOVICI, 1888). 2006. 72 f. Dissertação (Mestrado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2006. |
url |
http://locus.ufv.br/handle/123456789/5079 |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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dc.publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
dc.publisher.program.fl_str_mv |
Mestrado em Medicina Veterinária |
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UFV |
dc.publisher.country.fl_str_mv |
BR |
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Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de |
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Universidade Federal de Viçosa |
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