Genetic variability of Euphorbia yellow mosaic virus and Macroptilium yellow vein virus in their respective natural hosts, Euphorbia heterophylla and Macroptilium lathyroides

Detalhes bibliográficos
Autor(a) principal: Lemos, Pedro Paulo Ferreira
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/4417
Resumo: Begomoviruses (genus Begomovirus, family Geminiviridae) comprise a group of plant viruses of great economic importance causing serious economic losses in tropical and subtropical crops. It is believed that the emergence of begomoviruses in Brazil occurred through horizontal transfer of viruses previously restricted to non-cultivated plants by the B biotype of Bemisia tabaci. Little is known about the genetic variability of weedinfecting begomoviruses. The study of this variability is important to understand how viruses evolve in order to adopt strategies for the development of crop cultivars with durable resistance. In this study we investigated the genetic variability of two weedinfecting begomoviruses, Euphorbia yellow mosaic virus (EuYMV) and Macroptilium yellow vein virus (MaYVV), which infect Euphorbia heterophylla and Macroptilium lathyroides, respectively. Our results, based on 19 DNA-A sequences of EuYMV and 18 of MaYVV obtained from samples collected in 2011 and 2012, support the hypothesis that the genetic structure of begomoviruses can be modulated by their hosts by common processes of selection, mutation and recombination. We observed distinct degrees of genetic variability between the two viruses. EuYMV presented a higher variability, similar to other weed-infecting begomoviruses, while MaYVV presented a lower variability. The nucleotide diversity of EuYMV (0.00819) was four-fold higher than that of MaYVV (0.00197). The mutation frequency of EuYMV (2.5×10-3) was higher than that of MaYVV (4.2×10-4). This difference was supported by the higher nucleotide diversity of all genes of EuYMV compared to MaYVV: CP (~three-fold), Rep (~seven-fold), Trap (~32-fold), Ren (~four-fold), AC4 (~eight-fold). Therefore the higher variability of EuYMV can be explained mostly by the Trap gene. The lower diversity observed for MaYVV could be due to its recent emergence compared to EuYMV, reported since the 1950's in Brazil.
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spelling Lemos, Pedro Paulo Ferreirahttp://lattes.cnpq.br/4369220975212118Zerbini Júnior, Francisco Murilohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783743U5Urquiza, Gloria Patricia Castillohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4778353J2Zerbini, Poliane Alfenashttp://lattes.cnpq.br/86323281595330712015-03-26T13:37:50Z2013-11-222015-03-26T13:37:50Z2013-03-15LEMOS, Pedro Paulo Ferreira. Genetic variability of Euphorbia yellow mosaic virus and Macroptilium yellow vein virus in their respective natural hosts, Euphorbia heterophylla and Macroptilium lathyroides. 2013. 100 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2013.http://locus.ufv.br/handle/123456789/4417Begomoviruses (genus Begomovirus, family Geminiviridae) comprise a group of plant viruses of great economic importance causing serious economic losses in tropical and subtropical crops. It is believed that the emergence of begomoviruses in Brazil occurred through horizontal transfer of viruses previously restricted to non-cultivated plants by the B biotype of Bemisia tabaci. Little is known about the genetic variability of weedinfecting begomoviruses. The study of this variability is important to understand how viruses evolve in order to adopt strategies for the development of crop cultivars with durable resistance. In this study we investigated the genetic variability of two weedinfecting begomoviruses, Euphorbia yellow mosaic virus (EuYMV) and Macroptilium yellow vein virus (MaYVV), which infect Euphorbia heterophylla and Macroptilium lathyroides, respectively. Our results, based on 19 DNA-A sequences of EuYMV and 18 of MaYVV obtained from samples collected in 2011 and 2012, support the hypothesis that the genetic structure of begomoviruses can be modulated by their hosts by common processes of selection, mutation and recombination. We observed distinct degrees of genetic variability between the two viruses. EuYMV presented a higher variability, similar to other weed-infecting begomoviruses, while MaYVV presented a lower variability. The nucleotide diversity of EuYMV (0.00819) was four-fold higher than that of MaYVV (0.00197). The mutation frequency of EuYMV (2.5×10-3) was higher than that of MaYVV (4.2×10-4). This difference was supported by the higher nucleotide diversity of all genes of EuYMV compared to MaYVV: CP (~three-fold), Rep (~seven-fold), Trap (~32-fold), Ren (~four-fold), AC4 (~eight-fold). Therefore the higher variability of EuYMV can be explained mostly by the Trap gene. The lower diversity observed for MaYVV could be due to its recent emergence compared to EuYMV, reported since the 1950's in Brazil.Os begomovírus (gênero Begomovirus, familia Geminiviridae) são um grupo vírus de plantas de grande importância econômica causando sérias perdas em diversas culturas tropicais e subtropicais. Acredita-se que a emergência dos begomovírus presentes no Brasil se deu por meio da transferência horizontal de vírus anteriormente restritos a plantas silvestres e invasoras para plantas cultivadas mediada pelo biótipo B de Bemisia tabaci. A maioria dos trabalhos realizados até o presente tiveram enfoque na caracterização molecular dos begomovírus e pouco se sabe sobre a variabilidade genética das populações destes no campo. O estudo desta variabilidade é importante para entender como os vírus evoluem no sentido de serem adotadas estratégias para o desenvolvimento de cultivares com resistência durável. Neste trabalho foi investigada a variabilidade genética de dois begomovírus encontrados em plantas invasoras, Euphorbia yellow mosaic virus (EuYMV) e Macroptilium yellow vein virus (MaYVV), que infectam Euphorbia heterophylla e Macroptilium lathyroides, respectivamente. Os resultados, baseados em 19 sequências de EuYMV e 18 de MaYVV obtidas de amostras coletadas em 2011 e 2012, reforçam a hipótese de que a estrutura genética de begomovírus pode ser modulada pelo hospedeiro por processos comuns de seleção, mutação e recombinação. Foram observados diferentes graus de variabilidade genética entre os dois vírus. O EuYMV apresentou maior variabilidade, semelhante a outros begomovírus que infectam plantas não cultivadas, enquanto o MaYVV apresentou baixa variabilidade. A diversidade nucleotídica do EuYMV (0,00819) foi aproximadamente quatro vezes mais elevada do que a do MaYVV (0,00197). A frequência de mutação do EuYMV (2,5×10-3) foi superior à do MaYVV (4,2×10-4). Esta diferença foi suportada pela maior diversidade nucleotídica de todos os genes do EuYMV em comparação ao MaYVV: CP (aprox. três vezes maior), Rep (sete vezes), Trap (32 vezes), Ren (quatro vezes), AC4 (oito vezes). Esses resultados indicam que a maior variabilidade genética de EuYMV pode ser explicada, principalmente, pelo gene Trap. A menor variabilidade observada para o MaYVV pode ser devido à sua provável emergência recente em comparação ao EuYMV, relatado desde a década de 1950 no Brasil.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em FitopatologiaUFVBREtiologia; Epidemiologia; ControleBegomovírusVariabilidade genéticaGeminivírusFilogeniaRecombinaçãoBegomovirusesGenetic variabilityGeminivirusPhylogeny, RecombinationCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIAGenetic variability of Euphorbia yellow mosaic virus and Macroptilium yellow vein virus in their respective natural hosts, Euphorbia heterophylla and Macroptilium lathyroidesGenetic variability of Euphorbia yellow mosaic virus and Macroptilium yellow vein virus in their respective natural hosts, Euphorbia heterophylla and Macroptilium lathyroidesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf668759https://locus.ufv.br//bitstream/123456789/4417/1/texto%20completo.pdf554c480543a7e27e41087d744a247db0MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain186384https://locus.ufv.br//bitstream/123456789/4417/2/texto%20completo.pdf.txt16be2038220c87abc6284b578bebfc78MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3722https://locus.ufv.br//bitstream/123456789/4417/3/texto%20completo.pdf.jpge78d09eac3b15993120e5793473f0bd9MD53123456789/44172016-04-10 23:10:04.985oai:locus.ufv.br:123456789/4417Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:10:04LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Genetic variability of Euphorbia yellow mosaic virus and Macroptilium yellow vein virus in their respective natural hosts, Euphorbia heterophylla and Macroptilium lathyroides
dc.title.alternative.eng.fl_str_mv Genetic variability of Euphorbia yellow mosaic virus and Macroptilium yellow vein virus in their respective natural hosts, Euphorbia heterophylla and Macroptilium lathyroides
title Genetic variability of Euphorbia yellow mosaic virus and Macroptilium yellow vein virus in their respective natural hosts, Euphorbia heterophylla and Macroptilium lathyroides
spellingShingle Genetic variability of Euphorbia yellow mosaic virus and Macroptilium yellow vein virus in their respective natural hosts, Euphorbia heterophylla and Macroptilium lathyroides
Lemos, Pedro Paulo Ferreira
Begomovírus
Variabilidade genética
Geminivírus
Filogenia
Recombinação
Begomoviruses
Genetic variability
Geminivirus
Phylogeny, Recombination
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
title_short Genetic variability of Euphorbia yellow mosaic virus and Macroptilium yellow vein virus in their respective natural hosts, Euphorbia heterophylla and Macroptilium lathyroides
title_full Genetic variability of Euphorbia yellow mosaic virus and Macroptilium yellow vein virus in their respective natural hosts, Euphorbia heterophylla and Macroptilium lathyroides
title_fullStr Genetic variability of Euphorbia yellow mosaic virus and Macroptilium yellow vein virus in their respective natural hosts, Euphorbia heterophylla and Macroptilium lathyroides
title_full_unstemmed Genetic variability of Euphorbia yellow mosaic virus and Macroptilium yellow vein virus in their respective natural hosts, Euphorbia heterophylla and Macroptilium lathyroides
title_sort Genetic variability of Euphorbia yellow mosaic virus and Macroptilium yellow vein virus in their respective natural hosts, Euphorbia heterophylla and Macroptilium lathyroides
author Lemos, Pedro Paulo Ferreira
author_facet Lemos, Pedro Paulo Ferreira
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/4369220975212118
dc.contributor.author.fl_str_mv Lemos, Pedro Paulo Ferreira
dc.contributor.advisor1.fl_str_mv Zerbini Júnior, Francisco Murilo
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783743U5
dc.contributor.referee1.fl_str_mv Urquiza, Gloria Patricia Castillo
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4778353J2
dc.contributor.referee2.fl_str_mv Zerbini, Poliane Alfenas
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/8632328159533071
contributor_str_mv Zerbini Júnior, Francisco Murilo
Urquiza, Gloria Patricia Castillo
Zerbini, Poliane Alfenas
dc.subject.por.fl_str_mv Begomovírus
Variabilidade genética
Geminivírus
Filogenia
Recombinação
topic Begomovírus
Variabilidade genética
Geminivírus
Filogenia
Recombinação
Begomoviruses
Genetic variability
Geminivirus
Phylogeny, Recombination
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
dc.subject.eng.fl_str_mv Begomoviruses
Genetic variability
Geminivirus
Phylogeny, Recombination
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
description Begomoviruses (genus Begomovirus, family Geminiviridae) comprise a group of plant viruses of great economic importance causing serious economic losses in tropical and subtropical crops. It is believed that the emergence of begomoviruses in Brazil occurred through horizontal transfer of viruses previously restricted to non-cultivated plants by the B biotype of Bemisia tabaci. Little is known about the genetic variability of weedinfecting begomoviruses. The study of this variability is important to understand how viruses evolve in order to adopt strategies for the development of crop cultivars with durable resistance. In this study we investigated the genetic variability of two weedinfecting begomoviruses, Euphorbia yellow mosaic virus (EuYMV) and Macroptilium yellow vein virus (MaYVV), which infect Euphorbia heterophylla and Macroptilium lathyroides, respectively. Our results, based on 19 DNA-A sequences of EuYMV and 18 of MaYVV obtained from samples collected in 2011 and 2012, support the hypothesis that the genetic structure of begomoviruses can be modulated by their hosts by common processes of selection, mutation and recombination. We observed distinct degrees of genetic variability between the two viruses. EuYMV presented a higher variability, similar to other weed-infecting begomoviruses, while MaYVV presented a lower variability. The nucleotide diversity of EuYMV (0.00819) was four-fold higher than that of MaYVV (0.00197). The mutation frequency of EuYMV (2.5×10-3) was higher than that of MaYVV (4.2×10-4). This difference was supported by the higher nucleotide diversity of all genes of EuYMV compared to MaYVV: CP (~three-fold), Rep (~seven-fold), Trap (~32-fold), Ren (~four-fold), AC4 (~eight-fold). Therefore the higher variability of EuYMV can be explained mostly by the Trap gene. The lower diversity observed for MaYVV could be due to its recent emergence compared to EuYMV, reported since the 1950's in Brazil.
publishDate 2013
dc.date.available.fl_str_mv 2013-11-22
2015-03-26T13:37:50Z
dc.date.issued.fl_str_mv 2013-03-15
dc.date.accessioned.fl_str_mv 2015-03-26T13:37:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv LEMOS, Pedro Paulo Ferreira. Genetic variability of Euphorbia yellow mosaic virus and Macroptilium yellow vein virus in their respective natural hosts, Euphorbia heterophylla and Macroptilium lathyroides. 2013. 100 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2013.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/4417
identifier_str_mv LEMOS, Pedro Paulo Ferreira. Genetic variability of Euphorbia yellow mosaic virus and Macroptilium yellow vein virus in their respective natural hosts, Euphorbia heterophylla and Macroptilium lathyroides. 2013. 100 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2013.
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dc.publisher.department.fl_str_mv Etiologia; Epidemiologia; Controle
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