Isolation and regeneration of Penicillium brevicompactum protoplasts - DOI: 10.4025/actascibiolsci.v26i4.1529
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Publication Date: | 2008 |
Other Authors: | , , |
Format: | Article |
Language: | por |
Source: | Acta Scientiarum Biological Sciences |
Download full: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1529 |
Summary: | The isolation and regeneration of fungal protoplasts is a key step for the establishment of transformation systems, electrophoretic karyotype analysis and fusion between strains, all techniques of broad application on improvement programs of filamentous fungi. To establish conditions for the isolation and regeneration of ,em>Penicillium brevicompactum protoplasts, that is an excellent pectinase producer, different lytic enzymes and osmotic stabilizers were tested. P. brevicompactum protoplasts were obtained at a larger scale when their mycelium was digested with 15mg.mL-1 of Glucanex (Novo Nordisk) and 0.8 mol.L–1 NaCl as osmotic stabilizer. The best osmotic stabilizer for the regeneration of P. brevicompactum protoplasts was 0.8 mol.L–1 KCl, with a regeneration frequency of 36.58%. This protocol can be applied in genetic analysis of this Penicillium species which, to date has been poorly characterized |
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Isolation and regeneration of Penicillium brevicompactum protoplasts - DOI: 10.4025/actascibiolsci.v26i4.1529Isolamento e regeneração de protoplastos de Penicillium brevicompactum - DOI: 10.4025/actascibiolsci.v26i4.1529Penicilliumpectinasesprotoplastos2.01.00.00-0 Biologia GeralThe isolation and regeneration of fungal protoplasts is a key step for the establishment of transformation systems, electrophoretic karyotype analysis and fusion between strains, all techniques of broad application on improvement programs of filamentous fungi. To establish conditions for the isolation and regeneration of ,em>Penicillium brevicompactum protoplasts, that is an excellent pectinase producer, different lytic enzymes and osmotic stabilizers were tested. P. brevicompactum protoplasts were obtained at a larger scale when their mycelium was digested with 15mg.mL-1 of Glucanex (Novo Nordisk) and 0.8 mol.L–1 NaCl as osmotic stabilizer. The best osmotic stabilizer for the regeneration of P. brevicompactum protoplasts was 0.8 mol.L–1 KCl, with a regeneration frequency of 36.58%. This protocol can be applied in genetic analysis of this Penicillium species which, to date has been poorly characterizedO isolamento e regeneração de protoplastos de fungos é um passo fundamental para o estabelecimento de sistemas de transformação, análise do cariótipo molecular e fusão entre linhagens, que são técnicas de ampla aplicação em programas de melhoramento para fungos filamentosos. Neste trabalho foram testados diferentes preparações enzimáticas e estabilizadores osmóticos para estabelecer condições otimizadas de isolamento e regeneração de protoplastos de Penicillium brevicompactum, que é um excelente produtor de pectinases. Protoplastos de P. brevicompactum foram obtidos em maior quantidade quando o micélio foi digerido com 15mg.mL-1 de Glucanex (Novo Nordisk) em NaCl 0,8 mol.L-1 como estabilizador osmótico. O melhor estabilizador osmótico para a regeneração dos protoplastos foi KCl 0.8 mol.L–1 apresentando uma freqüência de regeneração de 36,58%. Esse protocolo pode ser utilizado em análises genéticas para essa espécie de Penicillium cujos estudos têm sido pouco reportadosUniversidade Estadual De Maringá2008-03-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/152910.4025/actascibiolsci.v26i4.1529Acta Scientiarum. Biological Sciences; Vol 26 No 4 (2004); 475-479Acta Scientiarum. Biological Sciences; v. 26 n. 4 (2004); 475-4791807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1529/957Varavalho, Maurílio AntônioQueiroz, Marisa Vieira dePereira, Jorge FernandoAraújo, Elza Fernandes deinfo:eu-repo/semantics/openAccess2022-11-23T17:33:12Zoai:periodicos.uem.br/ojs:article/1529Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-23T17:33:12Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Isolation and regeneration of Penicillium brevicompactum protoplasts - DOI: 10.4025/actascibiolsci.v26i4.1529 Isolamento e regeneração de protoplastos de Penicillium brevicompactum - DOI: 10.4025/actascibiolsci.v26i4.1529 |
title |
Isolation and regeneration of Penicillium brevicompactum protoplasts - DOI: 10.4025/actascibiolsci.v26i4.1529 |
spellingShingle |
Isolation and regeneration of Penicillium brevicompactum protoplasts - DOI: 10.4025/actascibiolsci.v26i4.1529 Varavalho, Maurílio Antônio Penicillium pectinases protoplastos 2.01.00.00-0 Biologia Geral |
title_short |
Isolation and regeneration of Penicillium brevicompactum protoplasts - DOI: 10.4025/actascibiolsci.v26i4.1529 |
title_full |
Isolation and regeneration of Penicillium brevicompactum protoplasts - DOI: 10.4025/actascibiolsci.v26i4.1529 |
title_fullStr |
Isolation and regeneration of Penicillium brevicompactum protoplasts - DOI: 10.4025/actascibiolsci.v26i4.1529 |
title_full_unstemmed |
Isolation and regeneration of Penicillium brevicompactum protoplasts - DOI: 10.4025/actascibiolsci.v26i4.1529 |
title_sort |
Isolation and regeneration of Penicillium brevicompactum protoplasts - DOI: 10.4025/actascibiolsci.v26i4.1529 |
author |
Varavalho, Maurílio Antônio |
author_facet |
Varavalho, Maurílio Antônio Queiroz, Marisa Vieira de Pereira, Jorge Fernando Araújo, Elza Fernandes de |
author_role |
author |
author2 |
Queiroz, Marisa Vieira de Pereira, Jorge Fernando Araújo, Elza Fernandes de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Varavalho, Maurílio Antônio Queiroz, Marisa Vieira de Pereira, Jorge Fernando Araújo, Elza Fernandes de |
dc.subject.por.fl_str_mv |
Penicillium pectinases protoplastos 2.01.00.00-0 Biologia Geral |
topic |
Penicillium pectinases protoplastos 2.01.00.00-0 Biologia Geral |
description |
The isolation and regeneration of fungal protoplasts is a key step for the establishment of transformation systems, electrophoretic karyotype analysis and fusion between strains, all techniques of broad application on improvement programs of filamentous fungi. To establish conditions for the isolation and regeneration of ,em>Penicillium brevicompactum protoplasts, that is an excellent pectinase producer, different lytic enzymes and osmotic stabilizers were tested. P. brevicompactum protoplasts were obtained at a larger scale when their mycelium was digested with 15mg.mL-1 of Glucanex (Novo Nordisk) and 0.8 mol.L–1 NaCl as osmotic stabilizer. The best osmotic stabilizer for the regeneration of P. brevicompactum protoplasts was 0.8 mol.L–1 KCl, with a regeneration frequency of 36.58%. This protocol can be applied in genetic analysis of this Penicillium species which, to date has been poorly characterized |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-03-31 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1529 10.4025/actascibiolsci.v26i4.1529 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1529 |
identifier_str_mv |
10.4025/actascibiolsci.v26i4.1529 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1529/957 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 26 No 4 (2004); 475-479 Acta Scientiarum. Biological Sciences; v. 26 n. 4 (2004); 475-479 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
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1799317392449339392 |