Obtenção e caracterização do perfil lipídico do óleo da semente de Plukenetia volubilis e seu efeito na composição lipídica do fígado e tecido adiposo e na histopatologia do tronco aórtico de camundongos C57BL/6 e apoE-/-

• Autor(a) principal: Castro, Eduardo França
• Data de Publicação: 2013
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/271
• topic: Aterosclerose; Plukenetia volubilis; Óleos vegetais; Ácidos graxos Ômega; Histopatologia; Atherosclerosis; Plukenetia volubilis; Vegetable oils; Omega fatty acids; Histopathology; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

Atherosclerosis is a disease characterized by inflammatory nature and formation of lesions in arteries of large and medium caliber. Currently, we seek to identify preventive measures that can reduce the prevalence of atherosclerosis, and among them is the consumption of polyunsaturated fatty acids, mainly family ω3 for its anti-inflammatory properties. In this sense, the oil sacha kiruma (Plukenetia volubilis) stands out for the high content of polyunsaturated fatty acids (84.67%), of which at least 48.42% belong to the family ω3. This study aimed to: (i) to characterize the lipid profile of the seeds of P. volubilis grown in Viçosa, MG, (ii) evaluate the effects of diet enriched with seed oil P. voubilis in mice of strains C57BL / 6 and apoE-/-. The oil, after extraction with hexane, had its lipid profile analyzed by gas chromatography. This oil was used to produce diets normolipídicas (7% / AIN-93G) that were offered for 90 days Black mice (n = 10) and apoE-/ - (n = 10) and was used for control diet enriched with soybean oil to Black mice (n = 10) and apoE-/- (n = 10). We evaluated food intake, body weight, lipid metabolism and morphological and histological aorta. We obtained the following results: (i) the lipid profile analysis revealed 6.29% of saturated fatty acids and a high content of polyunsaturated (86.49%), 51.18% of these are of ω3 and ω6 35.31% of (ii) treatment with oil sacha kiruma influenced the greater deposition of essential fatty acids (polyunsaturated ω3 and ω6) and lower ω3/ω6 relationship between abdominal fat and liver of mice and Black apoE -/-, which may have contributed to the protective effect of oil sacha kiruma both in the presence of fewer atherosclerotic lesions and absence of advanced lesions in the ascending aorta and terminals mouse apoE-/-. However, there is need for further studies involving diet enriched with oil sacha kiruma in atherosclerosis, as well as research aimed at selecting higher yielding genotypes and identify management techniques that enhance the quality and quantity of polyunsaturated fatty acids in their seeds.