Antibacterial activity of 7-epiclusianone and Its novel copper metal complex on Streptococcus spp. isolated from bovine mastitis and their cytotoxicity in MAC-T cells
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.3390/molecules22050823 http://www.locus.ufv.br/handle/123456789/12021 |
Resumo: | Mastitis is an inflammation of mammary gland parenchyma that adversely affects bovine health and dairy production worldwide despite significant efforts to eradicate it. The aim of this work was to characterize the antimicrobial activity of 7-epiclusianone (7-epi), a compound extracted from the Rheedia brasiliensis fruit, its complex with copper against Streptococcus spp. isolated from bovine mastitis, and to assess their cytotoxicity to bovine mammary alveolar cells (MAC-T). The complex 7-epiclusianone-Cu (7-epi-Cu) was an amorphous green solid with optical activity. Its vibrational spectrum in the infrared region showed absorption bands in the high-frequency region, as well as bands that can be attributed to the unconjugated and conjugated stretching of the free ligand. The complex was anhydrous. One of the tested bacterial strains was not sensitive to the compounds, while the other three had MIC values of 7.8 µg mL−1 and minimum bactericidal concentration (MBC) values between 15.6 and 31.3 µg mL−1. These two compounds are bacteriostatic, did not cause damage to the cell wall and, at sub-inhibitory concentrations, did not induce bacterial adhesion. The compounds were not cytotoxic. Based on these results, 7-epi and 7-epi-Cu exhibited desirable antimicrobial properties and could potentially be used in bovine mastitis treatment. |
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Barros, Mariana dePerciano, Pedro GriffoSantos, Marcelo Henrique dosOliveira, Leandro Licursi DeCosta, Éderson D’MartinMoreira, Maria Aparecida Scatamburlo2017-10-11T14:02:50Z2017-10-11T14:02:50Z2017-05-171420-3049http://dx.doi.org/10.3390/molecules22050823http://www.locus.ufv.br/handle/123456789/12021Mastitis is an inflammation of mammary gland parenchyma that adversely affects bovine health and dairy production worldwide despite significant efforts to eradicate it. The aim of this work was to characterize the antimicrobial activity of 7-epiclusianone (7-epi), a compound extracted from the Rheedia brasiliensis fruit, its complex with copper against Streptococcus spp. isolated from bovine mastitis, and to assess their cytotoxicity to bovine mammary alveolar cells (MAC-T). The complex 7-epiclusianone-Cu (7-epi-Cu) was an amorphous green solid with optical activity. Its vibrational spectrum in the infrared region showed absorption bands in the high-frequency region, as well as bands that can be attributed to the unconjugated and conjugated stretching of the free ligand. The complex was anhydrous. One of the tested bacterial strains was not sensitive to the compounds, while the other three had MIC values of 7.8 µg mL−1 and minimum bactericidal concentration (MBC) values between 15.6 and 31.3 µg mL−1. These two compounds are bacteriostatic, did not cause damage to the cell wall and, at sub-inhibitory concentrations, did not induce bacterial adhesion. The compounds were not cytotoxic. Based on these results, 7-epi and 7-epi-Cu exhibited desirable antimicrobial properties and could potentially be used in bovine mastitis treatment.engMolecules22(5), 823 20177-epiclusianoneMastitisBacterial activityCytotoxicityMAC-TStreptococcus spp.Rheedia brasiliensisAntibacterial activity of 7-epiclusianone and Its novel copper metal complex on Streptococcus spp. isolated from bovine mastitis and their cytotoxicity in MAC-T cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALmolecules-22-00823.pdfmolecules-22-00823.pdftexto completoapplication/pdf2755297https://locus.ufv.br//bitstream/123456789/12021/1/molecules-22-00823.pdf5cf3c2cbc1330b673917b8123133c4d7MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/12021/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILmolecules-22-00823.pdf.jpgmolecules-22-00823.pdf.jpgIM Thumbnailimage/jpeg2404https://locus.ufv.br//bitstream/123456789/12021/3/molecules-22-00823.pdf.jpg7b6979746c49271ad74603ccadbb0eabMD53123456789/120212017-10-11 23:00:52.498oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-10-12T02:00:52LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Antibacterial activity of 7-epiclusianone and Its novel copper metal complex on Streptococcus spp. isolated from bovine mastitis and their cytotoxicity in MAC-T cells |
title |
Antibacterial activity of 7-epiclusianone and Its novel copper metal complex on Streptococcus spp. isolated from bovine mastitis and their cytotoxicity in MAC-T cells |
spellingShingle |
Antibacterial activity of 7-epiclusianone and Its novel copper metal complex on Streptococcus spp. isolated from bovine mastitis and their cytotoxicity in MAC-T cells Barros, Mariana de 7-epiclusianone Mastitis Bacterial activity Cytotoxicity MAC-T Streptococcus spp. Rheedia brasiliensis |
title_short |
Antibacterial activity of 7-epiclusianone and Its novel copper metal complex on Streptococcus spp. isolated from bovine mastitis and their cytotoxicity in MAC-T cells |
title_full |
Antibacterial activity of 7-epiclusianone and Its novel copper metal complex on Streptococcus spp. isolated from bovine mastitis and their cytotoxicity in MAC-T cells |
title_fullStr |
Antibacterial activity of 7-epiclusianone and Its novel copper metal complex on Streptococcus spp. isolated from bovine mastitis and their cytotoxicity in MAC-T cells |
title_full_unstemmed |
Antibacterial activity of 7-epiclusianone and Its novel copper metal complex on Streptococcus spp. isolated from bovine mastitis and their cytotoxicity in MAC-T cells |
title_sort |
Antibacterial activity of 7-epiclusianone and Its novel copper metal complex on Streptococcus spp. isolated from bovine mastitis and their cytotoxicity in MAC-T cells |
author |
Barros, Mariana de |
author_facet |
Barros, Mariana de Perciano, Pedro Griffo Santos, Marcelo Henrique dos Oliveira, Leandro Licursi De Costa, Éderson D’Martin Moreira, Maria Aparecida Scatamburlo |
author_role |
author |
author2 |
Perciano, Pedro Griffo Santos, Marcelo Henrique dos Oliveira, Leandro Licursi De Costa, Éderson D’Martin Moreira, Maria Aparecida Scatamburlo |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Barros, Mariana de Perciano, Pedro Griffo Santos, Marcelo Henrique dos Oliveira, Leandro Licursi De Costa, Éderson D’Martin Moreira, Maria Aparecida Scatamburlo |
dc.subject.pt-BR.fl_str_mv |
7-epiclusianone Mastitis Bacterial activity Cytotoxicity MAC-T Streptococcus spp. Rheedia brasiliensis |
topic |
7-epiclusianone Mastitis Bacterial activity Cytotoxicity MAC-T Streptococcus spp. Rheedia brasiliensis |
description |
Mastitis is an inflammation of mammary gland parenchyma that adversely affects bovine health and dairy production worldwide despite significant efforts to eradicate it. The aim of this work was to characterize the antimicrobial activity of 7-epiclusianone (7-epi), a compound extracted from the Rheedia brasiliensis fruit, its complex with copper against Streptococcus spp. isolated from bovine mastitis, and to assess their cytotoxicity to bovine mammary alveolar cells (MAC-T). The complex 7-epiclusianone-Cu (7-epi-Cu) was an amorphous green solid with optical activity. Its vibrational spectrum in the infrared region showed absorption bands in the high-frequency region, as well as bands that can be attributed to the unconjugated and conjugated stretching of the free ligand. The complex was anhydrous. One of the tested bacterial strains was not sensitive to the compounds, while the other three had MIC values of 7.8 µg mL−1 and minimum bactericidal concentration (MBC) values between 15.6 and 31.3 µg mL−1. These two compounds are bacteriostatic, did not cause damage to the cell wall and, at sub-inhibitory concentrations, did not induce bacterial adhesion. The compounds were not cytotoxic. Based on these results, 7-epi and 7-epi-Cu exhibited desirable antimicrobial properties and could potentially be used in bovine mastitis treatment. |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-10-11T14:02:50Z |
dc.date.available.fl_str_mv |
2017-10-11T14:02:50Z |
dc.date.issued.fl_str_mv |
2017-05-17 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/molecules22050823 http://www.locus.ufv.br/handle/123456789/12021 |
dc.identifier.issn.none.fl_str_mv |
1420-3049 |
identifier_str_mv |
1420-3049 |
url |
http://dx.doi.org/10.3390/molecules22050823 http://www.locus.ufv.br/handle/123456789/12021 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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22(5), 823 2017 |
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Molecules |
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Molecules |
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