Efeitos dos flavonoides naringina, crisina e hesperidina e do extrato hidroalcoólico de Helianthus annuus na carcinogênese experimental do cólon

• Autor(a) principal: Sequetto, Priscila Lima
• Data de Publicação: 2012
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/314
• topic: Flavonoides; Helianthus annuus; Flavonoid; Helianthus annuus; CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::METABOLISMO E BIOENERGETICA

This study aimed to evaluate the effects of the flavonoid naringin, hesperidin and chrysin and extract of Helianthus annuus in experimental colon carcinogenesis in rats exposed to 1.2 dimethylhydrazine. In each experiment we used Wistar female weighing 27.1 g ± 130.8 received weekly subcutaneous injection of 1,2-dimethylhydrazine (DMH, 20 mg/kg) for 10 weeks. From week 11, treatment was started with the flavonoid naringin, chrysin, hesperidin and the hydroalcoholic extract of Helianthus annuus, administered orally three times a week every 48 hours for 14 weeks. The animals were divided into 7 groups with five animals in each group. Group 1: NaCl 0,9%, Group 2: DMH + NaCl 0,9%, Group 3: DMH + treatment (10 mg/Kg), Group 4: DMH + treatment (100 mg/Kg), Group 5: DMH + treatment (200 mg/Kg). The evaluation of the results was reaizada from analyzes to quantify the number of aberrant crypt foci (ACF), histopathology, immunohistochemistry and histomorphometry, evaluation of nucleolar organizer regions per nucleus of enterocytes and enteroendocrine cells AgNORs, serum biochemical analysis and microanalysis minerals. Overall, the induction and control groups treated with the lower dose significantly increased the number of dysplastic crypts with dilated FCA, of AgNORs/nucleus and reduction of antioxidant minerals such as copper, magnesium, selenium and zinc compared to the healthy group. In other dosages used were a decrease in colorectal morphological disorganization and a decrease in the number of precancerous lesions in rats exposed to the chemical carcinogen DMH. The early and middle segments of the colon were more sensitive to the inhibitory effect of these treatments on the intestinal epithelial dysplasia, which apparently have limited influence on pre-established tumors. It is believed that some of the results may be due to the effects of these treatments in reducing the proliferation and recovery levels of minerals antioxidants in colonic mucosa.