Efeitos dos flavonoides naringina, crisina e hesperidina e do extrato hidroalcoólico de Helianthus annuus na carcinogênese experimental do cólon
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/314 |
Resumo: | This study aimed to evaluate the effects of the flavonoid naringin, hesperidin and chrysin and extract of Helianthus annuus in experimental colon carcinogenesis in rats exposed to 1.2 dimethylhydrazine. In each experiment we used Wistar female weighing 27.1 g ± 130.8 received weekly subcutaneous injection of 1,2-dimethylhydrazine (DMH, 20 mg/kg) for 10 weeks. From week 11, treatment was started with the flavonoid naringin, chrysin, hesperidin and the hydroalcoholic extract of Helianthus annuus, administered orally three times a week every 48 hours for 14 weeks. The animals were divided into 7 groups with five animals in each group. Group 1: NaCl 0,9%, Group 2: DMH + NaCl 0,9%, Group 3: DMH + treatment (10 mg/Kg), Group 4: DMH + treatment (100 mg/Kg), Group 5: DMH + treatment (200 mg/Kg). The evaluation of the results was reaizada from analyzes to quantify the number of aberrant crypt foci (ACF), histopathology, immunohistochemistry and histomorphometry, evaluation of nucleolar organizer regions per nucleus of enterocytes and enteroendocrine cells AgNORs, serum biochemical analysis and microanalysis minerals. Overall, the induction and control groups treated with the lower dose significantly increased the number of dysplastic crypts with dilated FCA, of AgNORs/nucleus and reduction of antioxidant minerals such as copper, magnesium, selenium and zinc compared to the healthy group. In other dosages used were a decrease in colorectal morphological disorganization and a decrease in the number of precancerous lesions in rats exposed to the chemical carcinogen DMH. The early and middle segments of the colon were more sensitive to the inhibitory effect of these treatments on the intestinal epithelial dysplasia, which apparently have limited influence on pre-established tumors. It is believed that some of the results may be due to the effects of these treatments in reducing the proliferation and recovery levels of minerals antioxidants in colonic mucosa. |
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Sequetto, Priscila Limahttp://lattes.cnpq.br/2288558104248144Lamêgo, Márcia Rogéria de Almeidahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782197P4Nagem, Tanus Jorgehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788999D3Oliveira, Tânia Toledo dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787758J2Pinto, Aloísio da Silvahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4709052D4Silva, Marcelo Eustáquiohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788790E9Queiroz, José Humberto dehttp://lattes.cnpq.br/4881556650652069Mello, Vanessa Jóia dehttp://lattes.cnpq.br/94375892016897172015-03-26T12:15:19Z2013-04-172015-03-26T12:15:19Z2012-07-24SEQUETTO, Priscila Lima. Effects of the flavonoid naringin, chrysin and hesperidin and extract of Helianthus annuus in experimental colon carcinogenesis. 2012. 185 f. Tese (Doutorado em Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal) - Universidade Federal de Viçosa, Viçosa, 2012.http://locus.ufv.br/handle/123456789/314This study aimed to evaluate the effects of the flavonoid naringin, hesperidin and chrysin and extract of Helianthus annuus in experimental colon carcinogenesis in rats exposed to 1.2 dimethylhydrazine. In each experiment we used Wistar female weighing 27.1 g ± 130.8 received weekly subcutaneous injection of 1,2-dimethylhydrazine (DMH, 20 mg/kg) for 10 weeks. From week 11, treatment was started with the flavonoid naringin, chrysin, hesperidin and the hydroalcoholic extract of Helianthus annuus, administered orally three times a week every 48 hours for 14 weeks. The animals were divided into 7 groups with five animals in each group. Group 1: NaCl 0,9%, Group 2: DMH + NaCl 0,9%, Group 3: DMH + treatment (10 mg/Kg), Group 4: DMH + treatment (100 mg/Kg), Group 5: DMH + treatment (200 mg/Kg). The evaluation of the results was reaizada from analyzes to quantify the number of aberrant crypt foci (ACF), histopathology, immunohistochemistry and histomorphometry, evaluation of nucleolar organizer regions per nucleus of enterocytes and enteroendocrine cells AgNORs, serum biochemical analysis and microanalysis minerals. Overall, the induction and control groups treated with the lower dose significantly increased the number of dysplastic crypts with dilated FCA, of AgNORs/nucleus and reduction of antioxidant minerals such as copper, magnesium, selenium and zinc compared to the healthy group. In other dosages used were a decrease in colorectal morphological disorganization and a decrease in the number of precancerous lesions in rats exposed to the chemical carcinogen DMH. The early and middle segments of the colon were more sensitive to the inhibitory effect of these treatments on the intestinal epithelial dysplasia, which apparently have limited influence on pre-established tumors. It is believed that some of the results may be due to the effects of these treatments in reducing the proliferation and recovery levels of minerals antioxidants in colonic mucosa.Neste estudo, objetivou-se avaliar os efeitos dos flavonóides naringina, hesperidina e crisina e do extrato hidroalcoólico de Helianthus annuus na carcinogênese experimental do cólon de ratos expostos à 1,2 dimetilhidrazina. Em cada experimento, foram utilizados ratos Wistar fêmeas pesando 130,8 ± 27,1g que receberam injeção subcutânea semanal de 1,2-dimetilhidrazina (DMH, 20 mg/kg) durante 10 semanas. A partir da 11ª semana, foi iniciado o tratamento com os flavonóides naringina, crisina, hesperidina e com o extrato hidroalcoólico de Helianthus annuus, administrado por gavagem 3 vezes por semana a cada 48 horas, durante 14 semanas. Os animais foram divididos em 5 grupos com 7 animais em cada grupo. Grupo 1: NaCl 0,9%; Grupo 2: DMH + NaCl 0,9%; Grupo 3: DMH + tratamento (10 mg/Kg); Grupo 4: DMH + tratamento (100 mg/Kg); Grupo 5: DMH + tratamento (200 mg/Kg). A avaliação dos resultados foi reaizada a partir de análises de quantificação do número de focos de criptas aberrantes (FCA), histopatologia, histoquímica e histomorfometria, avaliação de regiões organizadora de nucléolo por núcleo de enterócitos AgNORs e de células enteroendócrinas, análise bioquímica sérica e microanálise de minerais. De forma geral, nos grupos controle da indução e tratado com a menor dose houve aumento significativo do número de FCA com criptas displásicas dilatadas, das AgNORs/núcleo e redução dos minerais antioxidantes como cobre, magnésio, selênio e zinco comparado ao grupo sadio. Nas outras dosagens utilizadas foi observada redução da desorganização morfológica colorretal, com a diminuição do número de lesões pré-neoplásicas em ratos expostos ao carcinógeno químico DMH. Os segmentos inicial e médio do cólon foram mais sensíveis ao efeito inibitório destes tratamentos sobre a displasia do epitélio intestinal, os quais aparentemente apresentam influência limitada sobre lesões tumorais previamente estabelecidas. Acredita-se que parte dos resultados encontrados pode ser decorrente dos efeitos destes tratamentos na redução da proliferação celular e recuperação dos níveis de minerais antioxidantes na mucosa intestinal.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaDoutorado em Bioquímica AgrícolaUFVBRBioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animalFlavonoidesHelianthus annuusFlavonoidHelianthus annuusCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::METABOLISMO E BIOENERGETICAEfeitos dos flavonoides naringina, crisina e hesperidina e do extrato hidroalcoólico de Helianthus annuus na carcinogênese experimental do cólonEffects of the flavonoid naringin, chrysin and hesperidin and extract of Helianthus annuus in experimental colon carcinogenesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf4685388https://locus.ufv.br//bitstream/123456789/314/1/texto%20completo.pdf8f207544a21e9420b42f876c48738b1eMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain282986https://locus.ufv.br//bitstream/123456789/314/2/texto%20completo.pdf.txtf4047baa27f8cc88d381fb44e5195b32MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3543https://locus.ufv.br//bitstream/123456789/314/3/texto%20completo.pdf.jpg6daa57d098076fe3f625b1a650eb1286MD53123456789/3142016-04-06 23:03:09.378oai:locus.ufv.br:123456789/314Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-07T02:03:09LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Efeitos dos flavonoides naringina, crisina e hesperidina e do extrato hidroalcoólico de Helianthus annuus na carcinogênese experimental do cólon |
dc.title.alternative.eng.fl_str_mv |
Effects of the flavonoid naringin, chrysin and hesperidin and extract of Helianthus annuus in experimental colon carcinogenesis |
title |
Efeitos dos flavonoides naringina, crisina e hesperidina e do extrato hidroalcoólico de Helianthus annuus na carcinogênese experimental do cólon |
spellingShingle |
Efeitos dos flavonoides naringina, crisina e hesperidina e do extrato hidroalcoólico de Helianthus annuus na carcinogênese experimental do cólon Sequetto, Priscila Lima Flavonoides Helianthus annuus Flavonoid Helianthus annuus CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::METABOLISMO E BIOENERGETICA |
title_short |
Efeitos dos flavonoides naringina, crisina e hesperidina e do extrato hidroalcoólico de Helianthus annuus na carcinogênese experimental do cólon |
title_full |
Efeitos dos flavonoides naringina, crisina e hesperidina e do extrato hidroalcoólico de Helianthus annuus na carcinogênese experimental do cólon |
title_fullStr |
Efeitos dos flavonoides naringina, crisina e hesperidina e do extrato hidroalcoólico de Helianthus annuus na carcinogênese experimental do cólon |
title_full_unstemmed |
Efeitos dos flavonoides naringina, crisina e hesperidina e do extrato hidroalcoólico de Helianthus annuus na carcinogênese experimental do cólon |
title_sort |
Efeitos dos flavonoides naringina, crisina e hesperidina e do extrato hidroalcoólico de Helianthus annuus na carcinogênese experimental do cólon |
author |
Sequetto, Priscila Lima |
author_facet |
Sequetto, Priscila Lima |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/2288558104248144 |
dc.contributor.author.fl_str_mv |
Sequetto, Priscila Lima |
dc.contributor.advisor-co1.fl_str_mv |
Lamêgo, Márcia Rogéria de Almeida |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782197P4 |
dc.contributor.advisor-co2.fl_str_mv |
Nagem, Tanus Jorge |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788999D3 |
dc.contributor.advisor1.fl_str_mv |
Oliveira, Tânia Toledo de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787758J2 |
dc.contributor.referee1.fl_str_mv |
Pinto, Aloísio da Silva |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4709052D4 |
dc.contributor.referee2.fl_str_mv |
Silva, Marcelo Eustáquio |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788790E9 |
dc.contributor.referee3.fl_str_mv |
Queiroz, José Humberto de |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/4881556650652069 |
dc.contributor.referee4.fl_str_mv |
Mello, Vanessa Jóia de |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/9437589201689717 |
contributor_str_mv |
Lamêgo, Márcia Rogéria de Almeida Nagem, Tanus Jorge Oliveira, Tânia Toledo de Pinto, Aloísio da Silva Silva, Marcelo Eustáquio Queiroz, José Humberto de Mello, Vanessa Jóia de |
dc.subject.por.fl_str_mv |
Flavonoides Helianthus annuus |
topic |
Flavonoides Helianthus annuus Flavonoid Helianthus annuus CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::METABOLISMO E BIOENERGETICA |
dc.subject.eng.fl_str_mv |
Flavonoid Helianthus annuus |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::METABOLISMO E BIOENERGETICA |
description |
This study aimed to evaluate the effects of the flavonoid naringin, hesperidin and chrysin and extract of Helianthus annuus in experimental colon carcinogenesis in rats exposed to 1.2 dimethylhydrazine. In each experiment we used Wistar female weighing 27.1 g ± 130.8 received weekly subcutaneous injection of 1,2-dimethylhydrazine (DMH, 20 mg/kg) for 10 weeks. From week 11, treatment was started with the flavonoid naringin, chrysin, hesperidin and the hydroalcoholic extract of Helianthus annuus, administered orally three times a week every 48 hours for 14 weeks. The animals were divided into 7 groups with five animals in each group. Group 1: NaCl 0,9%, Group 2: DMH + NaCl 0,9%, Group 3: DMH + treatment (10 mg/Kg), Group 4: DMH + treatment (100 mg/Kg), Group 5: DMH + treatment (200 mg/Kg). The evaluation of the results was reaizada from analyzes to quantify the number of aberrant crypt foci (ACF), histopathology, immunohistochemistry and histomorphometry, evaluation of nucleolar organizer regions per nucleus of enterocytes and enteroendocrine cells AgNORs, serum biochemical analysis and microanalysis minerals. Overall, the induction and control groups treated with the lower dose significantly increased the number of dysplastic crypts with dilated FCA, of AgNORs/nucleus and reduction of antioxidant minerals such as copper, magnesium, selenium and zinc compared to the healthy group. In other dosages used were a decrease in colorectal morphological disorganization and a decrease in the number of precancerous lesions in rats exposed to the chemical carcinogen DMH. The early and middle segments of the colon were more sensitive to the inhibitory effect of these treatments on the intestinal epithelial dysplasia, which apparently have limited influence on pre-established tumors. It is believed that some of the results may be due to the effects of these treatments in reducing the proliferation and recovery levels of minerals antioxidants in colonic mucosa. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-07-24 |
dc.date.available.fl_str_mv |
2013-04-17 2015-03-26T12:15:19Z |
dc.date.accessioned.fl_str_mv |
2015-03-26T12:15:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SEQUETTO, Priscila Lima. Effects of the flavonoid naringin, chrysin and hesperidin and extract of Helianthus annuus in experimental colon carcinogenesis. 2012. 185 f. Tese (Doutorado em Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal) - Universidade Federal de Viçosa, Viçosa, 2012. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/314 |
identifier_str_mv |
SEQUETTO, Priscila Lima. Effects of the flavonoid naringin, chrysin and hesperidin and extract of Helianthus annuus in experimental colon carcinogenesis. 2012. 185 f. Tese (Doutorado em Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal) - Universidade Federal de Viçosa, Viçosa, 2012. |
url |
http://locus.ufv.br/handle/123456789/314 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
dc.publisher.program.fl_str_mv |
Doutorado em Bioquímica Agrícola |
dc.publisher.initials.fl_str_mv |
UFV |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal |
publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
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reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
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Universidade Federal de Viçosa (UFV) |
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UFV |
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LOCUS Repositório Institucional da UFV |
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