Pre-Infection stages of Austropuccinia psidii in the epidermis of Eucalyptus hybrid leaves with different resistance levels

Detalhes bibliográficos
Autor(a) principal: Silva, Renata Ruiz
Data de Publicação: 2017
Outros Autores: Silva, André Costa da, Rodella, Roberto Antônio, Serrão, José Eduardo, Zanuncio, José Cola, Furtado, Edson Luiz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.3390/f8100362
http://www.locus.ufv.br/handle/123456789/17344
Resumo: Rust is a major Eucalyptus spp. disease, which is especially damaging for early-stage plants. The aim of this study was to verify the pre-infection process of Austropuccinia psidii (A. psidii) in the leaves of three phenological stages of Eucalyptus clones with different resistance levels. Plants from the hybrids of Eucalyptus urophylla × Eucalyptus grandis (E. grandis) with variable levels of resistance to this disease were used. The pathogen was inoculated in vitro on abaxial leaf discs of first, third, and fifth leaf stages and maintained under conditions suitable for disease development. Subsequently, samples from these discs were collected 24 and 120 h after inoculation and processed using scanning electron microscopy analysis. No symptoms were seen in any leaf stage of the resistant clone. Additionally, a low incidence of A. psidii germination (1.3–2%) and appressoria (0–0.5%) in three leaf stages was observed. However, the first leaf stage of the susceptible clone presented germination of large numbers of urediniospores (65%) with appressoria (55%) and degradation of the cuticle and wax. From the third stage, the percentage of germinated urediniospores (<15%) and appressoria (<2%) formation of this clone decreased. Protrusions on the leaf surface, associated with the pathogen, were observed on the first and third leaf stages of the resistant clone and on the fifth stage of the susceptible clone, suggesting a possible defensive plant reaction.
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spelling Silva, Renata RuizSilva, André Costa daRodella, Roberto AntônioSerrão, José EduardoZanuncio, José ColaFurtado, Edson Luiz2018-02-06T14:11:16Z2018-02-06T14:11:16Z2017-10-1719994907https://doi.org/10.3390/f8100362http://www.locus.ufv.br/handle/123456789/17344Rust is a major Eucalyptus spp. disease, which is especially damaging for early-stage plants. The aim of this study was to verify the pre-infection process of Austropuccinia psidii (A. psidii) in the leaves of three phenological stages of Eucalyptus clones with different resistance levels. Plants from the hybrids of Eucalyptus urophylla × Eucalyptus grandis (E. grandis) with variable levels of resistance to this disease were used. The pathogen was inoculated in vitro on abaxial leaf discs of first, third, and fifth leaf stages and maintained under conditions suitable for disease development. Subsequently, samples from these discs were collected 24 and 120 h after inoculation and processed using scanning electron microscopy analysis. No symptoms were seen in any leaf stage of the resistant clone. Additionally, a low incidence of A. psidii germination (1.3–2%) and appressoria (0–0.5%) in three leaf stages was observed. However, the first leaf stage of the susceptible clone presented germination of large numbers of urediniospores (65%) with appressoria (55%) and degradation of the cuticle and wax. From the third stage, the percentage of germinated urediniospores (<15%) and appressoria (<2%) formation of this clone decreased. Protrusions on the leaf surface, associated with the pathogen, were observed on the first and third leaf stages of the resistant clone and on the fifth stage of the susceptible clone, suggesting a possible defensive plant reaction.engForestsv. 8, n. 10, october 2017Eucalypt rustPathogenesisPhenological stage leavesPre-infection stageResistanceLeaf discsScanning electron microscopyPuccinia psidiiPre-Infection stages of Austropuccinia psidii in the epidermis of Eucalyptus hybrid leaves with different resistance levelsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf8879539https://locus.ufv.br//bitstream/123456789/17344/1/artigo.pdfdaebd204404f2868725211379db7a7e7MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/17344/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg2414https://locus.ufv.br//bitstream/123456789/17344/3/artigo.pdf.jpg9b5a30c7bf056e2f6b0503cad1a5edbcMD53123456789/173442018-02-06 22:01:02.897oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-02-07T01:01:02LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Pre-Infection stages of Austropuccinia psidii in the epidermis of Eucalyptus hybrid leaves with different resistance levels
title Pre-Infection stages of Austropuccinia psidii in the epidermis of Eucalyptus hybrid leaves with different resistance levels
spellingShingle Pre-Infection stages of Austropuccinia psidii in the epidermis of Eucalyptus hybrid leaves with different resistance levels
Silva, Renata Ruiz
Eucalypt rust
Pathogenesis
Phenological stage leaves
Pre-infection stage
Resistance
Leaf discs
Scanning electron microscopy
Puccinia psidii
title_short Pre-Infection stages of Austropuccinia psidii in the epidermis of Eucalyptus hybrid leaves with different resistance levels
title_full Pre-Infection stages of Austropuccinia psidii in the epidermis of Eucalyptus hybrid leaves with different resistance levels
title_fullStr Pre-Infection stages of Austropuccinia psidii in the epidermis of Eucalyptus hybrid leaves with different resistance levels
title_full_unstemmed Pre-Infection stages of Austropuccinia psidii in the epidermis of Eucalyptus hybrid leaves with different resistance levels
title_sort Pre-Infection stages of Austropuccinia psidii in the epidermis of Eucalyptus hybrid leaves with different resistance levels
author Silva, Renata Ruiz
author_facet Silva, Renata Ruiz
Silva, André Costa da
Rodella, Roberto Antônio
Serrão, José Eduardo
Zanuncio, José Cola
Furtado, Edson Luiz
author_role author
author2 Silva, André Costa da
Rodella, Roberto Antônio
Serrão, José Eduardo
Zanuncio, José Cola
Furtado, Edson Luiz
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Renata Ruiz
Silva, André Costa da
Rodella, Roberto Antônio
Serrão, José Eduardo
Zanuncio, José Cola
Furtado, Edson Luiz
dc.subject.pt-BR.fl_str_mv Eucalypt rust
Pathogenesis
Phenological stage leaves
Pre-infection stage
Resistance
Leaf discs
Scanning electron microscopy
Puccinia psidii
topic Eucalypt rust
Pathogenesis
Phenological stage leaves
Pre-infection stage
Resistance
Leaf discs
Scanning electron microscopy
Puccinia psidii
description Rust is a major Eucalyptus spp. disease, which is especially damaging for early-stage plants. The aim of this study was to verify the pre-infection process of Austropuccinia psidii (A. psidii) in the leaves of three phenological stages of Eucalyptus clones with different resistance levels. Plants from the hybrids of Eucalyptus urophylla × Eucalyptus grandis (E. grandis) with variable levels of resistance to this disease were used. The pathogen was inoculated in vitro on abaxial leaf discs of first, third, and fifth leaf stages and maintained under conditions suitable for disease development. Subsequently, samples from these discs were collected 24 and 120 h after inoculation and processed using scanning electron microscopy analysis. No symptoms were seen in any leaf stage of the resistant clone. Additionally, a low incidence of A. psidii germination (1.3–2%) and appressoria (0–0.5%) in three leaf stages was observed. However, the first leaf stage of the susceptible clone presented germination of large numbers of urediniospores (65%) with appressoria (55%) and degradation of the cuticle and wax. From the third stage, the percentage of germinated urediniospores (<15%) and appressoria (<2%) formation of this clone decreased. Protrusions on the leaf surface, associated with the pathogen, were observed on the first and third leaf stages of the resistant clone and on the fifth stage of the susceptible clone, suggesting a possible defensive plant reaction.
publishDate 2017
dc.date.issued.fl_str_mv 2017-10-17
dc.date.accessioned.fl_str_mv 2018-02-06T14:11:16Z
dc.date.available.fl_str_mv 2018-02-06T14:11:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv https://doi.org/10.3390/f8100362
http://www.locus.ufv.br/handle/123456789/17344
dc.identifier.issn.none.fl_str_mv 19994907
identifier_str_mv 19994907
url https://doi.org/10.3390/f8100362
http://www.locus.ufv.br/handle/123456789/17344
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv v. 8, n. 10, october 2017
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