Criopreservação de sementes e pólen de orquídeas

• Autor(a) principal: Carvalho, Virginia Silva
• Data de Publicação: 2006
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/1079
• topic: Criopreservação; Orquídea; Semente; Pólen; Vitrificação; Germoplasma vegetal; Orchidaceae; Criopreservation; Orchid; Seeds; Pollen; Vitrification; Plant germoplasm; Orchidaceae; CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA

A simple, reliable, and low-cost cryopreservation method was developed for mature seeds of Dendrobium Jaquelyn Thomas, D. Sena Red Thailand, D. Mini W/RL and D. BFC Pink and for pollen of D. Sena Red Thailand and D. Mini W/RL. Vitrification treatments performed prior to cryopreservation included exposure of seeds and pollen to plant vitrification solution (PVS2) at room temperature or at ice temperature for one to four or five hours before storage in liquid nitrogen. After a few days seeds and pollen were removed from cryopreservation and germination rates were evaluated. Seeds placed directly on liquid nitrogen did not germinate after removal from cryopreservation. For the treatments evaluated, the highest germination rate was observed in seeds exposed to PVS2 for 1 hour on ice. The vitrification has shown to be essential for the germination success and survival of seeds after removal from liquid nitrogen. Germinated seeds developed into normal plantlets. The pollen of D. Sena Red Thailand and D. Mini W/RL has shown high germination rates, above 60 % for all treatments and controls. The vitrification treatment has shown not to be essential for the germination success of pollen after the cryopreservation. The vitrification method, a simple and less time-consuming method of cryopreservation, could be appropriate and practical for conservation of many accessions of orchid seeds.