Interações intra- e intermoleculares dos begomovírus e seus efeitos na adaptação viral e na maquinaria celular do hospedeiro
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://locus.ufv.br/handle/123456789/1062 |
Resumo: | Begomoviruses (family Geminiviridae) have one or two genomic components, infect dicotyledonous plants and are naturally transmitted by the whitefly Bemisia tabaci (Homoptera: Aleyrodidae). Begomoviruses cause diseases of major economic importance in many crops, especially in tropical and subtropical regions. In Brazil, a viral complex composed of at least eight species, including Tomato rugose mosaic virus (ToRMV), Tomato severe rugose virus (ToSRV) and Tomato yellow spot virus (ToYSV), is responsible for losses in tomato. The complete DNA-B sequences of ToRMV and ToSRV show an identity of 98.2% and the high identity of their common region sequences (96.2%) indicates that the two viruses may share the same DNA-B. For the successful establishment of a viral infection, several interactions are required between begomovirus and host proteins. In this work, experiments were conducted with the following objectives: (1) to analyze the importance of recombination and pseudorecombination in the generation of variability and adaptation to the host of ToRMV and ToSRV; (2) to examine the involvement of two components of the anaphase-promoting complex (APC7 and APC10) in begomovirus replication; (3) to detect and characterize host proteins which interact with the ToYSV movement protein, MP. For the first objective, Nicotiana benthamiana plants were inoculated with all possible combinations of ToRMV and ToSRV DNA-A and DNA-B. Additionally, recombination and nucleotide/amino acid sequence comparisons between ToRMV and ToSRV were performed. The results indicate that ToRMV has both a recombinant and pseudorecombinant origin, and that ToRMV exerts a negative interference over ToSRV. For the second objective, Arabidopsis thaliana plants overexpressing APC7, APC10 and different APC7 mutants were inoculated with Cabbage leaf curl virus (CaLCuV), and viral DNA accumulation was estimated in plants at 14 and 28 days post-inoculation (dpi). The results indicate that APC7 and APC10 negatively affect CaLCuV DNA accumulation, and that both the N-terminal and C-terminal portions of the APC7 protein are involved in this effect. For the third objective, leaves of N. benthamiana were agroinfiltrated with the "bait" construct NTAPi-MP, and 48 hours after agroinfiltration the leaves were collected for extraction and purification of protein heterocomplexes. However, this transient expression assay in leaves of N. benthamiana failed to detect interactions between ToYSV MP and host proteins. |
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Silva, Fábio Nascimento dahttp://lattes.cnpq.br/1976091081462116Fontes, Elizabeth Pacheco Batistahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781848H2Zerbini, Poliane Alfenashttp://lattes.cnpq.br/8632328159533071Zerbini Júnior, Francisco Murilohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783743U5Carvalho, Claudine Márciahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794965T6Barros, Everaldo Gonçalves dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781285J6Ferreira, Paulo Cavalcanti Gomeshttp://lattes.cnpq.br/85355927013924252015-03-26T12:42:05Z2014-08-182015-03-26T12:42:05Z2011-02-28SILVA, Fábio Nascimento da. Intra- and intermolecular interactions of begomoviruses and their effects on viral adaptation and on the host cellular machinery.. 2011. 127 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2011.http://locus.ufv.br/handle/123456789/1062Begomoviruses (family Geminiviridae) have one or two genomic components, infect dicotyledonous plants and are naturally transmitted by the whitefly Bemisia tabaci (Homoptera: Aleyrodidae). Begomoviruses cause diseases of major economic importance in many crops, especially in tropical and subtropical regions. In Brazil, a viral complex composed of at least eight species, including Tomato rugose mosaic virus (ToRMV), Tomato severe rugose virus (ToSRV) and Tomato yellow spot virus (ToYSV), is responsible for losses in tomato. The complete DNA-B sequences of ToRMV and ToSRV show an identity of 98.2% and the high identity of their common region sequences (96.2%) indicates that the two viruses may share the same DNA-B. For the successful establishment of a viral infection, several interactions are required between begomovirus and host proteins. In this work, experiments were conducted with the following objectives: (1) to analyze the importance of recombination and pseudorecombination in the generation of variability and adaptation to the host of ToRMV and ToSRV; (2) to examine the involvement of two components of the anaphase-promoting complex (APC7 and APC10) in begomovirus replication; (3) to detect and characterize host proteins which interact with the ToYSV movement protein, MP. For the first objective, Nicotiana benthamiana plants were inoculated with all possible combinations of ToRMV and ToSRV DNA-A and DNA-B. Additionally, recombination and nucleotide/amino acid sequence comparisons between ToRMV and ToSRV were performed. The results indicate that ToRMV has both a recombinant and pseudorecombinant origin, and that ToRMV exerts a negative interference over ToSRV. For the second objective, Arabidopsis thaliana plants overexpressing APC7, APC10 and different APC7 mutants were inoculated with Cabbage leaf curl virus (CaLCuV), and viral DNA accumulation was estimated in plants at 14 and 28 days post-inoculation (dpi). The results indicate that APC7 and APC10 negatively affect CaLCuV DNA accumulation, and that both the N-terminal and C-terminal portions of the APC7 protein are involved in this effect. For the third objective, leaves of N. benthamiana were agroinfiltrated with the "bait" construct NTAPi-MP, and 48 hours after agroinfiltration the leaves were collected for extraction and purification of protein heterocomplexes. However, this transient expression assay in leaves of N. benthamiana failed to detect interactions between ToYSV MP and host proteins.Os begomovírus (família Geminiviridae) possuem um ou dois componentes genômicos, infectam plantas dicotiledôneas e são transmitidos naturalmente pela mosca- branca Bemisia tabaci (Homoptera:Aleyrodidae). Os begomovírus causam doenças de grande importância econômica em diversas culturas, principalmente em regiões tropicais e subtropicais. No Brasil, um complexo viral composto por pelo menos oito espécies, incluindo o Tomato rugose mosaic virus (ToRMV), Tomato severe rugose virus (ToSRV) e Tomato yellow spot virus (ToYSV), é responsável por grandes perdas na cultura do tomateiro. A sequência completa dos DNAs-B do ToSRV e ToRMV apresenta identidade de 98%, e a elevada identidade de sequência da região comum (96,2%) indica que os dois vírus podem compartilhar o mesmo DNA-B. Para o estabelecimento de uma infecção viral é necessária uma série de interações entre proteínas dos begomovírus e do hospedeiro. Neste trabalho, foram conduzidos experimentos com os objetivos de: (1) analisar a importância da recombinação e da pseudo-recombinação na geração de variabilidade e na adaptação ao hospedeiro dos begomovírus ToRMV e ToSRV; (2) analisar o envolvimento de APC7 e APC10, dois componentes do complexo promotor da anáfase, na replicação de begomovírus; (3) detectar e caracterizar proteína(s) do hospedeiro que interagem com a MP do begomovírus ToYSV. Para o primeiro objetivo, plantas de N. benthamiana foram inoculadas com todas as combinações possíveis entre o DNA-A e o DNA-B do ToRMV e do ToSRV. Análises de recombinação e das sequências de nucleotídeos e aminoácidos entre ToRMV e ToSRV também foram realizadas. Os resultados indicaram que o ToRMV apresenta uma origem recombinante e pseudo-recombinante, e que o ToRMV exerce uma interferência negativa sobre o ToSRV. Para o segundo objetivo, plantas de Arabidopsis thaliana superexpressando APC7 ou APC10 e diferentes mutantes para APC7 foram inoculadas com o begomovírus Cabbage leaf curl virus (CaLCuV) e o acúmulo de DNA viral foi estimado em plantas infectadas, aos 14 e 28 dias pós-inoculação (dpi). Os resultados indicaram que APC7 e APC10 afetam o acúmulo do CaLCuV, e que tanto a região N-terminal quanto a C-terminal de APC7 estão envolvidas neste efeito. Para o terceiro objetivo, folhas de N. benthamiana foram agroinfiltradas com a construção "isca" NTAPi-MP, e 48 horas após a agroinfiltração estas folhas foram coletadas para extração e purificação do heterocomplexo protéico. Entretanto, esse ensaio de expressão transiente em folhas de N. benthamiana não permitiu detectar interações entre a proteína MP do ToYSV e proteína(s) do hospedeiro.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de ViçosaDoutorado em FitopatologiaUFVBREtiologia; Epidemiologia; ControleBegomovírusAdaptação viralHospedeiroBegomovirusesViral adaptationHostCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIAInterações intra- e intermoleculares dos begomovírus e seus efeitos na adaptação viral e na maquinaria celular do hospedeiroIntra- and intermolecular interactions of begomoviruses and their effects on viral adaptation and on the host cellular machinery.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf1674702https://locus.ufv.br//bitstream/123456789/1062/1/texto%20completo.pdfab48c56e0fe4a5a3fc7dfa63a5d9e325MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain256214https://locus.ufv.br//bitstream/123456789/1062/2/texto%20completo.pdf.txt0d33ecce80f79e8b4eb384504a98c0a8MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3688https://locus.ufv.br//bitstream/123456789/1062/3/texto%20completo.pdf.jpgaff490ffcb8f4c6070512a7661da2cedMD53123456789/10622016-04-06 23:17:18.805oai:locus.ufv.br:123456789/1062Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-07T02:17:18LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Interações intra- e intermoleculares dos begomovírus e seus efeitos na adaptação viral e na maquinaria celular do hospedeiro |
dc.title.alternative.eng.fl_str_mv |
Intra- and intermolecular interactions of begomoviruses and their effects on viral adaptation and on the host cellular machinery. |
title |
Interações intra- e intermoleculares dos begomovírus e seus efeitos na adaptação viral e na maquinaria celular do hospedeiro |
spellingShingle |
Interações intra- e intermoleculares dos begomovírus e seus efeitos na adaptação viral e na maquinaria celular do hospedeiro Silva, Fábio Nascimento da Begomovírus Adaptação viral Hospedeiro Begomoviruses Viral adaptation Host CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA |
title_short |
Interações intra- e intermoleculares dos begomovírus e seus efeitos na adaptação viral e na maquinaria celular do hospedeiro |
title_full |
Interações intra- e intermoleculares dos begomovírus e seus efeitos na adaptação viral e na maquinaria celular do hospedeiro |
title_fullStr |
Interações intra- e intermoleculares dos begomovírus e seus efeitos na adaptação viral e na maquinaria celular do hospedeiro |
title_full_unstemmed |
Interações intra- e intermoleculares dos begomovírus e seus efeitos na adaptação viral e na maquinaria celular do hospedeiro |
title_sort |
Interações intra- e intermoleculares dos begomovírus e seus efeitos na adaptação viral e na maquinaria celular do hospedeiro |
author |
Silva, Fábio Nascimento da |
author_facet |
Silva, Fábio Nascimento da |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/1976091081462116 |
dc.contributor.author.fl_str_mv |
Silva, Fábio Nascimento da |
dc.contributor.advisor-co1.fl_str_mv |
Fontes, Elizabeth Pacheco Batista |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781848H2 |
dc.contributor.advisor-co2.fl_str_mv |
Zerbini, Poliane Alfenas |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://lattes.cnpq.br/8632328159533071 |
dc.contributor.advisor1.fl_str_mv |
Zerbini Júnior, Francisco Murilo |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783743U5 |
dc.contributor.referee1.fl_str_mv |
Carvalho, Claudine Márcia |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794965T6 |
dc.contributor.referee2.fl_str_mv |
Barros, Everaldo Gonçalves de |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781285J6 |
dc.contributor.referee3.fl_str_mv |
Ferreira, Paulo Cavalcanti Gomes |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/8535592701392425 |
contributor_str_mv |
Fontes, Elizabeth Pacheco Batista Zerbini, Poliane Alfenas Zerbini Júnior, Francisco Murilo Carvalho, Claudine Márcia Barros, Everaldo Gonçalves de Ferreira, Paulo Cavalcanti Gomes |
dc.subject.por.fl_str_mv |
Begomovírus Adaptação viral Hospedeiro |
topic |
Begomovírus Adaptação viral Hospedeiro Begomoviruses Viral adaptation Host CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA |
dc.subject.eng.fl_str_mv |
Begomoviruses Viral adaptation Host |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA |
description |
Begomoviruses (family Geminiviridae) have one or two genomic components, infect dicotyledonous plants and are naturally transmitted by the whitefly Bemisia tabaci (Homoptera: Aleyrodidae). Begomoviruses cause diseases of major economic importance in many crops, especially in tropical and subtropical regions. In Brazil, a viral complex composed of at least eight species, including Tomato rugose mosaic virus (ToRMV), Tomato severe rugose virus (ToSRV) and Tomato yellow spot virus (ToYSV), is responsible for losses in tomato. The complete DNA-B sequences of ToRMV and ToSRV show an identity of 98.2% and the high identity of their common region sequences (96.2%) indicates that the two viruses may share the same DNA-B. For the successful establishment of a viral infection, several interactions are required between begomovirus and host proteins. In this work, experiments were conducted with the following objectives: (1) to analyze the importance of recombination and pseudorecombination in the generation of variability and adaptation to the host of ToRMV and ToSRV; (2) to examine the involvement of two components of the anaphase-promoting complex (APC7 and APC10) in begomovirus replication; (3) to detect and characterize host proteins which interact with the ToYSV movement protein, MP. For the first objective, Nicotiana benthamiana plants were inoculated with all possible combinations of ToRMV and ToSRV DNA-A and DNA-B. Additionally, recombination and nucleotide/amino acid sequence comparisons between ToRMV and ToSRV were performed. The results indicate that ToRMV has both a recombinant and pseudorecombinant origin, and that ToRMV exerts a negative interference over ToSRV. For the second objective, Arabidopsis thaliana plants overexpressing APC7, APC10 and different APC7 mutants were inoculated with Cabbage leaf curl virus (CaLCuV), and viral DNA accumulation was estimated in plants at 14 and 28 days post-inoculation (dpi). The results indicate that APC7 and APC10 negatively affect CaLCuV DNA accumulation, and that both the N-terminal and C-terminal portions of the APC7 protein are involved in this effect. For the third objective, leaves of N. benthamiana were agroinfiltrated with the "bait" construct NTAPi-MP, and 48 hours after agroinfiltration the leaves were collected for extraction and purification of protein heterocomplexes. However, this transient expression assay in leaves of N. benthamiana failed to detect interactions between ToYSV MP and host proteins. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-02-28 |
dc.date.available.fl_str_mv |
2014-08-18 2015-03-26T12:42:05Z |
dc.date.accessioned.fl_str_mv |
2015-03-26T12:42:05Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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dc.identifier.citation.fl_str_mv |
SILVA, Fábio Nascimento da. Intra- and intermolecular interactions of begomoviruses and their effects on viral adaptation and on the host cellular machinery.. 2011. 127 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2011. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/1062 |
identifier_str_mv |
SILVA, Fábio Nascimento da. Intra- and intermolecular interactions of begomoviruses and their effects on viral adaptation and on the host cellular machinery.. 2011. 127 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2011. |
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http://locus.ufv.br/handle/123456789/1062 |
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por |
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Universidade Federal de Viçosa |
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Doutorado em Fitopatologia |
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UFV |
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BR |
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Etiologia; Epidemiologia; Controle |
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Universidade Federal de Viçosa |
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