Parâmetros enzimáticos e de tecidos de codornas alimentadas com dietas contendo diferentes relações de treonina com lisina

• Autor(a) principal: Barbosa, Anderson de Almeida
• Data de Publicação: 2009
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/295
• topic: Dieta; Codorna; Treonina; Diet; Quails; Threonine; CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::NUTRICAO E ALIMENTACAO ANIMAL

The work was consisted of two experiments. In the first, it was aimed to determine the effect of the age on the profile of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and in the liver biometry of laying quails (Coturnix coturnix japonica) from 1 to 25 days of age. In the second experiment, it was aimed to determine the effect of age and the variation of the diatery digestible threonine:digestible lysine ratios in activity of liver and serum AST and ALT, liver biometry, liver and serum total proteins and serum albumin and globulins. For the initial experiment, the experimental designed was completely randomized, with six ages and five replicates, being each animal an experimental unit. At 1, 5, 10, 15, 20 and 25 days of age five animals were sacrificed and livers were collected, weighted, frozen in liquid nitrogen and stored to -20ºC. For the secondexperiment, 60 quails of 57 days old were used and distributed in split splots. The digestible threonine:digestible lysine ratios were 0.65, 0.75 and 0.85. At the end of each experimental period, one bird of each replicate was selected and sacrificed by cervical dislocation. Blood and liver were immediately weighted. To approximately 100 mg of the liver were added 10 mL of distillated and deionizated cold water and homogenized with Potter-Ev mixer. The samples were centrifuged 7500 x g for 10 minutes and the supernatant used for determinations of the activities of hepatic ALT and AST. The serum was obtained after blood clotting at room temperature for 15 minutes. After the samples were centrifuged 7500 x g, for 10 minutes, and the supernatant used to determine the activities of AST and ALT. The liver and serum total proteins were determine by the Lowry method at 700 nm. For the first experiment the weight of the liver presented linear growth in function of age and it not increase in the same proportion of corporal weight in the same growth period. The total activities of AST and ALT presented linear growth in function of x the age. AST presented higher total activity in relation to the ALT. At the first day of age AST and ALT presented considerable specific activities in relation older ages. AST activity per gram of liver in function of the age was much higher than ALT in all ages. ALT per gram of corporal weight presented linear decrease in function of age. For the second experiment, the variation of the digestible threonine:digestible lysine ratios in the diet interfered in the amount of serum total proteins, albumin and globulins. Digestible threonine:digestible lysine ratio 0.85 provided better result for albumin levels. For serum AST and ALT activities, the 0.75 digestible threonine: digestible lysine ratios produced smaller activity for both enzymes. Liver AST, presented higher activity 0.75 ratio characterizing a metabolic adjustment. In conclusion the ratio recommended was 0.75.