Estudo de alguns marcadores inflamatórios e de síndrome metabólica em adolescentes com sobrepeso e naquelas metabolicamente obesas de peso normal

• Autor(a) principal: Carvalho, Gisele Queiroz
• Data de Publicação: 2008
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2698
• topic: Síndrome metabólica; Obesidade; Adolescentes; Metabolismo; Resistência à insulina; Metabolic syndrome; Obesity; Adolescents; Metabolism; Insulin resistance; CNPQ::CIENCIAS DA SAUDE::NUTRICAO

The subclinical chronic inflammation is confirmed as a new syndrome related to the development of insulin resistance. The fat tissue appears as important organ responsible for the increased expression of inflammatory substances, contributing to the development of this syndrome. This condition in adolescents with excess body fat is little known. The aim of this study was to evaluate the inflammatory profile of adolescents in overweight female post-pubescent, in those metabolic obese normal weight adolescents resistant to insulin. It was also a goal to verify the relationship between peripheral expression of mediators TNF-alpha, IL-6 and IL-10 and the parameters related to metabolic syndrome. 72 female adolescents in post-pubescent were evaluated, 14 to 17 years old. They were divided into 3 groups: eutrophic (G1), metabolic obese normal weight (G2) and overweight (G3). Data related to the parameters of the metabolic syndrome were collected: waist circumference, fasting plasma glucose, fasting insulin, insulin resistance, triglycerides, HDL, systolic and diastolic blood pressure. After the measurement of biochemical parameters, 10 overweight adolescents which were not insulin resistant were selected and those formed the control group (Si), and 10 overweight adolescents with insulin resistance were also chosen (Ri). It was evaluated the expression of mediators TNF-alpha, IL-6 and IL-10 in groups of study, and the correlation of these cytokines with the parameters of the metabolic syndrome. It was observed that G2 had intermediary values of weight, IMC and %GC in comparison to G1 and G3. Glicemia and insulin resistance were increased in G2 in comparison to G1, not differing of the G3. Insulinemia was increased and HDL was decreased in G2 in comparison to G3. Evaluating the peripheral expression of citocinas of G2 and G3 in comparison to G1, G3 had increase of 6% and 31% in expression of TNF-α and IL-10, respectively, and G2 had increase of 44% in expression of IL-6, without differences in the expression of cytokines between G2 and G3. There was positive correlation between TNF-α and IL-6, and between IL-10 and pro-inflammatory cytokines in the adolescents of G2, and in the adolescents with excess of body fat (G2 + G3), and positive correlation between IL-6 and glicemia (G2 group), and between IL-6 and triglycerides (G3 group). Considering overweight adolescents, there was no difference in the expression of cytokines evaluated among Si and Ri groups, although peripheric expression of IL-10 in the Ri group was 370% larger, regarding the Si group. In the adolescents of the Si group, IL-6 was positively correlated with IL-10, and negatively with insulinemia. IL-10 was negatively correlated with fast glicemia in the Si group. In the Ri group, there was no correlation between expression of cytokines and the parameters related with the metabolic syndrome. In the present study, Metabolic obese normal weight adolescents had metabolic profile, inflammatory profile and body composition similar to adolescents with overweight. It demonstrated the possible relation between IL-6 and glicemia and triglycerides. The positive correlations between IL-10 and TNF-α, IL-10 and IL-6, as well as the increased expression of IL-10 in overweigh adolescents regarding the eutrophic, suggests an attempt to inhibition the production of these citocinas by IL-10. Overweight adolescents with insulin resistance had similar peripehric expression to those overweight adolescents without insulin resistance. The increase of IL-10, and their negative correlation with fast glicêmica, can indicate possible protective paper of this cytokine in the development of cardiovascular risk factors.