Avaliações bioquímicas, hemogasométricas e morfológicas do sangue canino conservado em bolsas CPD/SAG-M e CPD/SAG-M acrescidas de fosfato dissódico

• Autor(a) principal: Costa Júnior, Jair Duarte da
• Data de Publicação: 2010
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/1439
• topic: Hematologia; Sangue; Fosfato dissódico; Coleta; Transfusão; Hematology; Blood; Disodium phosphate; Collection; Transfusion; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA

For many decades, studies have been developed in order to improve the biochemical and hemgasometric parameters of erythrocytes stored in bags of blood transfusion. Although there are still indications for the use of total blood, nowadays the balls are centrifuged and the blood components are separated to obtain in the erythrocytes concentrations (EC) and plasma. The viability of EC preserved by refrigeration is dependent on several factors. Among them the anticoagulant and the preservative solution added to the EC. Studies have shown that the addition of disodium phosphate (DP) preserves the pH in the best way, keeping it at higher levels which acts directly on the maintenance of adenosine triphosphate (ATP), a component involved in the preservation of the plasma membrane and thereby maintaining the normal shape of the cell and 2,3-DPG (2,3-DPG), a substance responsible for the release of oxygen to the tissues by hemoglobin. Methodology: In this study it was observed the biochemical and blood gas EC stored in CPD/SAG-M bags and CPD/SAG-M's added from DP. The bags were evaluated for 60 days. Results and Discussion: It was observed that the mediums of plasma potassium showed lower growth in the group treated with DP compared to the untreated group, even showing inversely proportional correlation to the behavior of pH in which it was observed averages always higher in the group added with the DP. Thus the best performance of the bags increased with DP in the maintenance of plasma membrane can be noticed. This finding is confirmed by a lower percentage of hemolysis suffered by the EC retained the balls containing the DP throughout the entire study. The consistently higher pH levels for the balls added with the FD show a higher rate of 2,3-DPG recovery, which showed higher average in the group treated with the DP. However, in the first day of the storage, the averages of 2,3-DPG were at zero, reaching maximum values at the 10th day of storage, demonstrating the capacity of producing 2,3-DPG in the canine erythrocytes even during the conservation process. The red cells preserved with DP showed lower morphological changes compared to the untreated group, also demonstrating a significant correlation with the potassium and 2,3-DPG variables, allowing to assume the average values of these biochemical variables from the morphological evaluation of stored erythrocytes. The erythrocytes of both groups showed an increase of the average diameter in the first day of the study, also with higher averages than those found in in vivo cells. From then it began to show reduction of the average diameter. It was concluded that the addition of DP to the CPD/SAG-M bags enhanced the preservation of erythrocyte up to the maximum period of 60 days of storage.