Partição de proteínas de soro de leite em sistemas aquosos bifásicos baseados em líquidos iônicos

Bibliographic Details
Main Author: Michel, Benôit
Publication Date: 2015
Other Authors: Neves, Mateus Tomaz, Sousa, Rita de Cássia Superbi de, Chagas, Marcelo Martins das, Martins, Bruna Amaral, Coimbra, Jane Sélia dos Reis
Format: Article
Language: por
Source: LOCUS Repositório Institucional da UFV
Download full: http://dx.doi.org/10.5935/0100-4042.20150123
http://www.locus.ufv.br/handle/123456789/24972
Summary: In this work, we used an aqueous two-phase system (ATPS) consisting of the ionic liquid [C4mim][Cl] and the salt K2HPO4 to partition α-lactalbumin, β-lactoglobulin, and lactoferrin whey proteins. Extraction efficiency values above 87% indicate that the proteins primarily migrated to the ionic liquid-rich phase (top phase). Partition coefficient values ranged from 6.17 to 8.93 for a-lactalbumin, from 22.80 to 34.55 for β-lactoglobulin, and from 26.46 to 40.06 for lactoferrin. Therefore, the saline ATPS with the ionic liquid examined in this study can be considered to be a promising alternative for extracting whey proteins.
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spelling Michel, BenôitNeves, Mateus TomazSousa, Rita de Cássia Superbi deChagas, Marcelo Martins dasMartins, Bruna AmaralCoimbra, Jane Sélia dos Reis2019-05-03T13:02:38Z2019-05-03T13:02:38Z2015-111678-7064http://dx.doi.org/10.5935/0100-4042.20150123http://www.locus.ufv.br/handle/123456789/24972In this work, we used an aqueous two-phase system (ATPS) consisting of the ionic liquid [C4mim][Cl] and the salt K2HPO4 to partition α-lactalbumin, β-lactoglobulin, and lactoferrin whey proteins. Extraction efficiency values above 87% indicate that the proteins primarily migrated to the ionic liquid-rich phase (top phase). Partition coefficient values ranged from 6.17 to 8.93 for a-lactalbumin, from 22.80 to 34.55 for β-lactoglobulin, and from 26.46 to 40.06 for lactoferrin. Therefore, the saline ATPS with the ionic liquid examined in this study can be considered to be a promising alternative for extracting whey proteins.porQuímica NovaV. 38, N. 9, P. 1148-1152, Nov. 2015Liquid–liquid extractionIonic liquidPartition coefficientProteinsPartição de proteínas de soro de leite em sistemas aquosos bifásicos baseados em líquidos iônicosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf162872https://locus.ufv.br//bitstream/123456789/24972/1/artigo.pdf491d7d30b999b4347d63f7398bbfbafdMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/24972/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/249722019-05-03 10:06:41.306oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452019-05-03T13:06:41LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.pt-BR.fl_str_mv Partição de proteínas de soro de leite em sistemas aquosos bifásicos baseados em líquidos iônicos
title Partição de proteínas de soro de leite em sistemas aquosos bifásicos baseados em líquidos iônicos
spellingShingle Partição de proteínas de soro de leite em sistemas aquosos bifásicos baseados em líquidos iônicos
Michel, Benôit
Liquid–liquid extraction
Ionic liquid
Partition coefficient
Proteins
title_short Partição de proteínas de soro de leite em sistemas aquosos bifásicos baseados em líquidos iônicos
title_full Partição de proteínas de soro de leite em sistemas aquosos bifásicos baseados em líquidos iônicos
title_fullStr Partição de proteínas de soro de leite em sistemas aquosos bifásicos baseados em líquidos iônicos
title_full_unstemmed Partição de proteínas de soro de leite em sistemas aquosos bifásicos baseados em líquidos iônicos
title_sort Partição de proteínas de soro de leite em sistemas aquosos bifásicos baseados em líquidos iônicos
author Michel, Benôit
author_facet Michel, Benôit
Neves, Mateus Tomaz
Sousa, Rita de Cássia Superbi de
Chagas, Marcelo Martins das
Martins, Bruna Amaral
Coimbra, Jane Sélia dos Reis
author_role author
author2 Neves, Mateus Tomaz
Sousa, Rita de Cássia Superbi de
Chagas, Marcelo Martins das
Martins, Bruna Amaral
Coimbra, Jane Sélia dos Reis
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Michel, Benôit
Neves, Mateus Tomaz
Sousa, Rita de Cássia Superbi de
Chagas, Marcelo Martins das
Martins, Bruna Amaral
Coimbra, Jane Sélia dos Reis
dc.subject.pt-BR.fl_str_mv Liquid–liquid extraction
Ionic liquid
Partition coefficient
Proteins
topic Liquid–liquid extraction
Ionic liquid
Partition coefficient
Proteins
description In this work, we used an aqueous two-phase system (ATPS) consisting of the ionic liquid [C4mim][Cl] and the salt K2HPO4 to partition α-lactalbumin, β-lactoglobulin, and lactoferrin whey proteins. Extraction efficiency values above 87% indicate that the proteins primarily migrated to the ionic liquid-rich phase (top phase). Partition coefficient values ranged from 6.17 to 8.93 for a-lactalbumin, from 22.80 to 34.55 for β-lactoglobulin, and from 26.46 to 40.06 for lactoferrin. Therefore, the saline ATPS with the ionic liquid examined in this study can be considered to be a promising alternative for extracting whey proteins.
publishDate 2015
dc.date.issued.fl_str_mv 2015-11
dc.date.accessioned.fl_str_mv 2019-05-03T13:02:38Z
dc.date.available.fl_str_mv 2019-05-03T13:02:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.5935/0100-4042.20150123
http://www.locus.ufv.br/handle/123456789/24972
dc.identifier.issn.none.fl_str_mv 1678-7064
identifier_str_mv 1678-7064
url http://dx.doi.org/10.5935/0100-4042.20150123
http://www.locus.ufv.br/handle/123456789/24972
dc.language.iso.fl_str_mv por
language por
dc.relation.ispartofseries.pt-BR.fl_str_mv V. 38, N. 9, P. 1148-1152, Nov. 2015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Química Nova
publisher.none.fl_str_mv Química Nova
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
bitstream.url.fl_str_mv https://locus.ufv.br//bitstream/123456789/24972/1/artigo.pdf
https://locus.ufv.br//bitstream/123456789/24972/2/license.txt
bitstream.checksum.fl_str_mv 491d7d30b999b4347d63f7398bbfbafd
8a4605be74aa9ea9d79846c1fba20a33
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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