Imunidade passiva em eqüinos neonatos: avaliação por diferentes métodos

• Autor(a) principal: Lang, André
• Data de Publicação: 2006
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/5203
• topic: Eqüino; Neonatologia; Anticorpos; Sorologia; Colostro; Equine; Neonatology; Immunity; Serum; Colostrum; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL::CLINICA VETERINARIA

The objective of this study was to evaluate passive transfer of immunity in newborn foals, by applying the single radial immunodiffusion (SRID) method and to verify immunoglobulin G correlation in the maternal serum, colostrum, and neonatal serum, as well as compare the results from this methodology with those from zinc sulfate turbidity (ZST) and gluteraldehyde coagulation (GC). Thus, 34 Breton cross bred animals were used, 17 mares and respective neonates, mixed Breton breed. The births were monitored and colostrum samples were collected immediately and 12 and 48 hours after birth. Blood samples were collected from the mares next to the foals birth and from the foals immediately, before colostrums ingestion, and 6, 12, 24, 48 hours and 30 days after birth. The samples were processed and IgG concentration determined in the colostrum by SRID. Blood serum samples was used for SRID, TSZ and GC. A drop in antibody concentration in the colostrum was observed after birth, however still significant values were detected within 48 hours. No significant correlation was verified between mare serum IgG concentration and respective colostral concentration, but rather between mare serum and neonate serum concentrations, at the moment that each animal presented maximum value of this immunoglobulin, which occurred between 6 and 24 hours after birth. Correlation was also found between IgG concentration in the colostral and neonatal serum at birth, and 12 and 48 hours after it. Maximum IgG values were found 48 hours after birth, for SRID, ZST and GC test. The comparison of the mean IgG values obtained each time indicated a period of 12 hours after birth as the ideal time for determining IgG concentration by the SRID and ZST techniques and of 6 hours using the GC test. A significant correlation was observed between IgG concentration obtained in the foal serum by the SRID technique with that found using ZST and GC test.