The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
DOI: | 10.1007/s13213-014-0873-4 |
Texto Completo: | https://doi.org/10.1007/s13213-014-0873-4 http://www.locus.ufv.br/handle/123456789/21602 |
Resumo: | The expression of the Penicillium griseoroseum plg1 gene is induced by citric pectin and repressed by glucose. In this work, the minimal region of the plg1 gene promoter essential for expression in pectin and sucrose plus yeast extract was identified by using constructs containing the gfp ORF under control of the plg1 gene promoter. The fragment A (283 bp) is essential for plg1 expression in sucrose plus yeast extract. Fragment B (309 bp plus 184; core promoter) was critical for expression in pectin and abolished the catabolic repression by glucose. Therefore, the fragment of 776 bp (fragment A and B) is essential for the expression of the plg1 gene in natural inducing conditions (pectin as carbon source) and in sucrose plus yeast extract. The fragment B is a promising minimal promoter usable for heterologous expression in filamentous fungi, since genes that contain it could be activated by the presence of peel from citric fruits (which contains citric pectin) and are not affected by glucose in these agricultural by-products. |
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The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 genePenicillium griseoroseumPectin lyaseMinimal promoter regionGreen fluorescent proteinThe expression of the Penicillium griseoroseum plg1 gene is induced by citric pectin and repressed by glucose. In this work, the minimal region of the plg1 gene promoter essential for expression in pectin and sucrose plus yeast extract was identified by using constructs containing the gfp ORF under control of the plg1 gene promoter. The fragment A (283 bp) is essential for plg1 expression in sucrose plus yeast extract. Fragment B (309 bp plus 184; core promoter) was critical for expression in pectin and abolished the catabolic repression by glucose. Therefore, the fragment of 776 bp (fragment A and B) is essential for the expression of the plg1 gene in natural inducing conditions (pectin as carbon source) and in sucrose plus yeast extract. The fragment B is a promising minimal promoter usable for heterologous expression in filamentous fungi, since genes that contain it could be activated by the presence of peel from citric fruits (which contains citric pectin) and are not affected by glucose in these agricultural by-products.Annals of Microbiology2018-09-04T10:49:04Z2018-09-04T10:49:04Z2015-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf18692044https://doi.org/10.1007/s13213-014-0873-4http://www.locus.ufv.br/handle/123456789/21602engv. 65, n. 2, p. 1145– 1148, june 2015Springer-Verlag Berlin Heidelberg and the University of Milaninfo:eu-repo/semantics/openAccessBazzolli, Denise Mara SoaresReis, Klédna Constância PortesTeixeira, Janaina AparecidaRibon, Andréa Oliveira BarrosQueiroz, Marisa Vieira deAraújo, Elza Fernandes dereponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T07:34:44Zoai:locus.ufv.br:123456789/21602Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:34:44LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.none.fl_str_mv |
The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene |
title |
The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene |
spellingShingle |
The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene Bazzolli, Denise Mara Soares Penicillium griseoroseum Pectin lyase Minimal promoter region Green fluorescent protein Bazzolli, Denise Mara Soares Penicillium griseoroseum Pectin lyase Minimal promoter region Green fluorescent protein |
title_short |
The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene |
title_full |
The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene |
title_fullStr |
The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene |
title_full_unstemmed |
The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene |
title_sort |
The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene |
author |
Bazzolli, Denise Mara Soares |
author_facet |
Bazzolli, Denise Mara Soares Bazzolli, Denise Mara Soares Reis, Klédna Constância Portes Teixeira, Janaina Aparecida Ribon, Andréa Oliveira Barros Queiroz, Marisa Vieira de Araújo, Elza Fernandes de Reis, Klédna Constância Portes Teixeira, Janaina Aparecida Ribon, Andréa Oliveira Barros Queiroz, Marisa Vieira de Araújo, Elza Fernandes de |
author_role |
author |
author2 |
Reis, Klédna Constância Portes Teixeira, Janaina Aparecida Ribon, Andréa Oliveira Barros Queiroz, Marisa Vieira de Araújo, Elza Fernandes de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Bazzolli, Denise Mara Soares Reis, Klédna Constância Portes Teixeira, Janaina Aparecida Ribon, Andréa Oliveira Barros Queiroz, Marisa Vieira de Araújo, Elza Fernandes de |
dc.subject.por.fl_str_mv |
Penicillium griseoroseum Pectin lyase Minimal promoter region Green fluorescent protein |
topic |
Penicillium griseoroseum Pectin lyase Minimal promoter region Green fluorescent protein |
description |
The expression of the Penicillium griseoroseum plg1 gene is induced by citric pectin and repressed by glucose. In this work, the minimal region of the plg1 gene promoter essential for expression in pectin and sucrose plus yeast extract was identified by using constructs containing the gfp ORF under control of the plg1 gene promoter. The fragment A (283 bp) is essential for plg1 expression in sucrose plus yeast extract. Fragment B (309 bp plus 184; core promoter) was critical for expression in pectin and abolished the catabolic repression by glucose. Therefore, the fragment of 776 bp (fragment A and B) is essential for the expression of the plg1 gene in natural inducing conditions (pectin as carbon source) and in sucrose plus yeast extract. The fragment B is a promising minimal promoter usable for heterologous expression in filamentous fungi, since genes that contain it could be activated by the presence of peel from citric fruits (which contains citric pectin) and are not affected by glucose in these agricultural by-products. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06 2018-09-04T10:49:04Z 2018-09-04T10:49:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
18692044 https://doi.org/10.1007/s13213-014-0873-4 http://www.locus.ufv.br/handle/123456789/21602 |
identifier_str_mv |
18692044 |
url |
https://doi.org/10.1007/s13213-014-0873-4 http://www.locus.ufv.br/handle/123456789/21602 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
v. 65, n. 2, p. 1145– 1148, june 2015 |
dc.rights.driver.fl_str_mv |
Springer-Verlag Berlin Heidelberg and the University of Milan info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Springer-Verlag Berlin Heidelberg and the University of Milan |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
pdf application/pdf |
dc.publisher.none.fl_str_mv |
Annals of Microbiology |
publisher.none.fl_str_mv |
Annals of Microbiology |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1822218854360678400 |
dc.identifier.doi.none.fl_str_mv |
10.1007/s13213-014-0873-4 |