The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene

Detalhes bibliográficos
Autor(a) principal: Bazzolli, Denise Mara Soares
Data de Publicação: 2015
Outros Autores: Reis, Klédna Constância Portes, Teixeira, Janaina Aparecida, Ribon, Andréa Oliveira Barros, Queiroz, Marisa Vieira de, Araújo, Elza Fernandes de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
DOI: 10.1007/s13213-014-0873-4
Texto Completo: https://doi.org/10.1007/s13213-014-0873-4
http://www.locus.ufv.br/handle/123456789/21602
Resumo: The expression of the Penicillium griseoroseum plg1 gene is induced by citric pectin and repressed by glucose. In this work, the minimal region of the plg1 gene promoter essential for expression in pectin and sucrose plus yeast extract was identified by using constructs containing the gfp ORF under control of the plg1 gene promoter. The fragment A (283 bp) is essential for plg1 expression in sucrose plus yeast extract. Fragment B (309 bp plus 184; core promoter) was critical for expression in pectin and abolished the catabolic repression by glucose. Therefore, the fragment of 776 bp (fragment A and B) is essential for the expression of the plg1 gene in natural inducing conditions (pectin as carbon source) and in sucrose plus yeast extract. The fragment B is a promising minimal promoter usable for heterologous expression in filamentous fungi, since genes that contain it could be activated by the presence of peel from citric fruits (which contains citric pectin) and are not affected by glucose in these agricultural by-products.
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spelling The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 genePenicillium griseoroseumPectin lyaseMinimal promoter regionGreen fluorescent proteinThe expression of the Penicillium griseoroseum plg1 gene is induced by citric pectin and repressed by glucose. In this work, the minimal region of the plg1 gene promoter essential for expression in pectin and sucrose plus yeast extract was identified by using constructs containing the gfp ORF under control of the plg1 gene promoter. The fragment A (283 bp) is essential for plg1 expression in sucrose plus yeast extract. Fragment B (309 bp plus 184; core promoter) was critical for expression in pectin and abolished the catabolic repression by glucose. Therefore, the fragment of 776 bp (fragment A and B) is essential for the expression of the plg1 gene in natural inducing conditions (pectin as carbon source) and in sucrose plus yeast extract. The fragment B is a promising minimal promoter usable for heterologous expression in filamentous fungi, since genes that contain it could be activated by the presence of peel from citric fruits (which contains citric pectin) and are not affected by glucose in these agricultural by-products.Annals of Microbiology2018-09-04T10:49:04Z2018-09-04T10:49:04Z2015-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf18692044https://doi.org/10.1007/s13213-014-0873-4http://www.locus.ufv.br/handle/123456789/21602engv. 65, n. 2, p. 1145– 1148, june 2015Springer-Verlag Berlin Heidelberg and the University of Milaninfo:eu-repo/semantics/openAccessBazzolli, Denise Mara SoaresReis, Klédna Constância PortesTeixeira, Janaina AparecidaRibon, Andréa Oliveira BarrosQueiroz, Marisa Vieira deAraújo, Elza Fernandes dereponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T07:34:44Zoai:locus.ufv.br:123456789/21602Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:34:44LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene
title The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene
spellingShingle The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene
The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene
Bazzolli, Denise Mara Soares
Penicillium griseoroseum
Pectin lyase
Minimal promoter region
Green fluorescent protein
Bazzolli, Denise Mara Soares
Penicillium griseoroseum
Pectin lyase
Minimal promoter region
Green fluorescent protein
title_short The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene
title_full The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene
title_fullStr The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene
The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene
title_full_unstemmed The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene
The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene
title_sort The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene
author Bazzolli, Denise Mara Soares
author_facet Bazzolli, Denise Mara Soares
Bazzolli, Denise Mara Soares
Reis, Klédna Constância Portes
Teixeira, Janaina Aparecida
Ribon, Andréa Oliveira Barros
Queiroz, Marisa Vieira de
Araújo, Elza Fernandes de
Reis, Klédna Constância Portes
Teixeira, Janaina Aparecida
Ribon, Andréa Oliveira Barros
Queiroz, Marisa Vieira de
Araújo, Elza Fernandes de
author_role author
author2 Reis, Klédna Constância Portes
Teixeira, Janaina Aparecida
Ribon, Andréa Oliveira Barros
Queiroz, Marisa Vieira de
Araújo, Elza Fernandes de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Bazzolli, Denise Mara Soares
Reis, Klédna Constância Portes
Teixeira, Janaina Aparecida
Ribon, Andréa Oliveira Barros
Queiroz, Marisa Vieira de
Araújo, Elza Fernandes de
dc.subject.por.fl_str_mv Penicillium griseoroseum
Pectin lyase
Minimal promoter region
Green fluorescent protein
topic Penicillium griseoroseum
Pectin lyase
Minimal promoter region
Green fluorescent protein
description The expression of the Penicillium griseoroseum plg1 gene is induced by citric pectin and repressed by glucose. In this work, the minimal region of the plg1 gene promoter essential for expression in pectin and sucrose plus yeast extract was identified by using constructs containing the gfp ORF under control of the plg1 gene promoter. The fragment A (283 bp) is essential for plg1 expression in sucrose plus yeast extract. Fragment B (309 bp plus 184; core promoter) was critical for expression in pectin and abolished the catabolic repression by glucose. Therefore, the fragment of 776 bp (fragment A and B) is essential for the expression of the plg1 gene in natural inducing conditions (pectin as carbon source) and in sucrose plus yeast extract. The fragment B is a promising minimal promoter usable for heterologous expression in filamentous fungi, since genes that contain it could be activated by the presence of peel from citric fruits (which contains citric pectin) and are not affected by glucose in these agricultural by-products.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
2018-09-04T10:49:04Z
2018-09-04T10:49:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 18692044
https://doi.org/10.1007/s13213-014-0873-4
http://www.locus.ufv.br/handle/123456789/21602
identifier_str_mv 18692044
url https://doi.org/10.1007/s13213-014-0873-4
http://www.locus.ufv.br/handle/123456789/21602
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv v. 65, n. 2, p. 1145– 1148, june 2015
dc.rights.driver.fl_str_mv Springer-Verlag Berlin Heidelberg and the University of Milan
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Springer-Verlag Berlin Heidelberg and the University of Milan
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Annals of Microbiology
publisher.none.fl_str_mv Annals of Microbiology
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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dc.identifier.doi.none.fl_str_mv 10.1007/s13213-014-0873-4