Overexpression of the plg1 gene encoding pectin lyase in Penicillium griseoroseum
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1007/s10295-007-0277-6 http://www.locus.ufv.br/handle/123456789/22280 |
Resumo: | The pectin lyase (PL) is an industrially important enzyme since it is used for maceration and clarification in the process of fruit juice production in food industries. In order to increase the yields of pectin lyase we cloned the plg1 (pectin lyase 1) from Penicillium griseoroseum gene under the control of the strong constitutive promoter of the glyceraldehyde-3-phosphate dehydrogenase gene (gpdA) and the terminator region of the tryptophan synthetase (trpC) gene from Aspergillus nidulans (plasmid pAN52-Plg1) and transformed this construct into the P. griseoroseum strain PG63. One of the pAN52-Plg1 multi-copy transformants (strain 105) grown in culture medium containing glucose or sugar cane juice showed PL activities of 4,804 or 5,202 U ml−1 respectively, which represented 57- and 132-fold increases. In addition, the apparent specific activity of PL produced by this strain was much higher than the one observed for a commercial pectinase preparation. Evaluation of the extracellular proteins in the culture supernatant of strain 105 by SDS-PAGE showed the presence of a clear and strong band of approximately 40 kDa that probably corresponds to PL. The enzyme yields reported here demonstrate that the system we developed is able to express pectin lyase at levels comparable to, or exceeding, previously reported data. |
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Overexpression of the plg1 gene encoding pectin lyase in Penicillium griseoroseumPenicillium griseoroseumPectin lyase genesTransformationOverexpressionGpd promoterThe pectin lyase (PL) is an industrially important enzyme since it is used for maceration and clarification in the process of fruit juice production in food industries. In order to increase the yields of pectin lyase we cloned the plg1 (pectin lyase 1) from Penicillium griseoroseum gene under the control of the strong constitutive promoter of the glyceraldehyde-3-phosphate dehydrogenase gene (gpdA) and the terminator region of the tryptophan synthetase (trpC) gene from Aspergillus nidulans (plasmid pAN52-Plg1) and transformed this construct into the P. griseoroseum strain PG63. One of the pAN52-Plg1 multi-copy transformants (strain 105) grown in culture medium containing glucose or sugar cane juice showed PL activities of 4,804 or 5,202 U ml−1 respectively, which represented 57- and 132-fold increases. In addition, the apparent specific activity of PL produced by this strain was much higher than the one observed for a commercial pectinase preparation. Evaluation of the extracellular proteins in the culture supernatant of strain 105 by SDS-PAGE showed the presence of a clear and strong band of approximately 40 kDa that probably corresponds to PL. The enzyme yields reported here demonstrate that the system we developed is able to express pectin lyase at levels comparable to, or exceeding, previously reported data.Journal of Industrial Microbiology & Biotechnology2018-10-16T12:10:38Z2018-10-16T12:10:38Z2007-11-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf14765535http://dx.doi.org/10.1007/s10295-007-0277-6http://www.locus.ufv.br/handle/123456789/22280engv. 35, n. 3, p. 159- 166, mar. 2008Society for Industrial Microbiologyinfo:eu-repo/semantics/openAccessTeixeira, Janaina AparecidaRibeiro, João BatistaTeixeira, Janaina AparecidaQueiroz, Marisa Vieira deAraújo, Elza Fernandes dereponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T07:53:23Zoai:locus.ufv.br:123456789/22280Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:53:23LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.none.fl_str_mv |
Overexpression of the plg1 gene encoding pectin lyase in Penicillium griseoroseum |
title |
Overexpression of the plg1 gene encoding pectin lyase in Penicillium griseoroseum |
spellingShingle |
Overexpression of the plg1 gene encoding pectin lyase in Penicillium griseoroseum Teixeira, Janaina Aparecida Penicillium griseoroseum Pectin lyase genes Transformation Overexpression Gpd promoter |
title_short |
Overexpression of the plg1 gene encoding pectin lyase in Penicillium griseoroseum |
title_full |
Overexpression of the plg1 gene encoding pectin lyase in Penicillium griseoroseum |
title_fullStr |
Overexpression of the plg1 gene encoding pectin lyase in Penicillium griseoroseum |
title_full_unstemmed |
Overexpression of the plg1 gene encoding pectin lyase in Penicillium griseoroseum |
title_sort |
Overexpression of the plg1 gene encoding pectin lyase in Penicillium griseoroseum |
author |
Teixeira, Janaina Aparecida |
author_facet |
Teixeira, Janaina Aparecida Ribeiro, João Batista Queiroz, Marisa Vieira de Araújo, Elza Fernandes de |
author_role |
author |
author2 |
Ribeiro, João Batista Queiroz, Marisa Vieira de Araújo, Elza Fernandes de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Teixeira, Janaina Aparecida Ribeiro, João Batista Teixeira, Janaina Aparecida Queiroz, Marisa Vieira de Araújo, Elza Fernandes de |
dc.subject.por.fl_str_mv |
Penicillium griseoroseum Pectin lyase genes Transformation Overexpression Gpd promoter |
topic |
Penicillium griseoroseum Pectin lyase genes Transformation Overexpression Gpd promoter |
description |
The pectin lyase (PL) is an industrially important enzyme since it is used for maceration and clarification in the process of fruit juice production in food industries. In order to increase the yields of pectin lyase we cloned the plg1 (pectin lyase 1) from Penicillium griseoroseum gene under the control of the strong constitutive promoter of the glyceraldehyde-3-phosphate dehydrogenase gene (gpdA) and the terminator region of the tryptophan synthetase (trpC) gene from Aspergillus nidulans (plasmid pAN52-Plg1) and transformed this construct into the P. griseoroseum strain PG63. One of the pAN52-Plg1 multi-copy transformants (strain 105) grown in culture medium containing glucose or sugar cane juice showed PL activities of 4,804 or 5,202 U ml−1 respectively, which represented 57- and 132-fold increases. In addition, the apparent specific activity of PL produced by this strain was much higher than the one observed for a commercial pectinase preparation. Evaluation of the extracellular proteins in the culture supernatant of strain 105 by SDS-PAGE showed the presence of a clear and strong band of approximately 40 kDa that probably corresponds to PL. The enzyme yields reported here demonstrate that the system we developed is able to express pectin lyase at levels comparable to, or exceeding, previously reported data. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-11-12 2018-10-16T12:10:38Z 2018-10-16T12:10:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
14765535 http://dx.doi.org/10.1007/s10295-007-0277-6 http://www.locus.ufv.br/handle/123456789/22280 |
identifier_str_mv |
14765535 |
url |
http://dx.doi.org/10.1007/s10295-007-0277-6 http://www.locus.ufv.br/handle/123456789/22280 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
v. 35, n. 3, p. 159- 166, mar. 2008 |
dc.rights.driver.fl_str_mv |
Society for Industrial Microbiology info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Society for Industrial Microbiology |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
pdf application/pdf |
dc.publisher.none.fl_str_mv |
Journal of Industrial Microbiology & Biotechnology |
publisher.none.fl_str_mv |
Journal of Industrial Microbiology & Biotechnology |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1817559969471397888 |