Expanded-bed adsorption utilizing ion-exchange resin to purify extracellular β-galactosidase

Detalhes bibliográficos
Autor(a) principal: Pereira, José Antonio Marques
Data de Publicação: 1998
Outros Autores: Rosa, Paulo De Tarso Vieira E., Pastore, Glaucia Maria, Santana, Cesar Costapinto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1007/BF02920188
http://www.locus.ufv.br/handle/123456789/19616
Resumo: The application of expanded-bed ion-exchange resins allows the elimination of intermediary particulate separation steps like filtration or centrifugation prior to adsorption steps in enzyme-purification processes from crude fermentation broths. This work is concerned with the experimental evaluation data of a process related to the adsorption of an extracellular p-galactosidase from the fungiScopulariopsis. The protein recovery in the ion-exchange resin Accell Plus QMA™ was accomplished using a continuous-monitoring method. The direct adsorption step was followed by a elution step with concentrated NaCl solutions aiming to improve the enzyme-specific activity. Experimental data for fixed and expanded bed were compared
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spelling Pereira, José Antonio MarquesRosa, Paulo De Tarso Vieira E.Pastore, Glaucia MariaSantana, Cesar Costapinto2018-05-16T13:55:59Z2018-05-16T13:55:59Z1998-0315590291https://doi.org/10.1007/BF02920188http://www.locus.ufv.br/handle/123456789/19616The application of expanded-bed ion-exchange resins allows the elimination of intermediary particulate separation steps like filtration or centrifugation prior to adsorption steps in enzyme-purification processes from crude fermentation broths. This work is concerned with the experimental evaluation data of a process related to the adsorption of an extracellular p-galactosidase from the fungiScopulariopsis. The protein recovery in the ion-exchange resin Accell Plus QMA™ was accomplished using a continuous-monitoring method. The direct adsorption step was followed by a elution step with concentrated NaCl solutions aiming to improve the enzyme-specific activity. Experimental data for fixed and expanded bed were comparedengApplied Biochemistry and Biotechnologyv. 70, Issue 1, p. 779–787, March 1998Humana Press Inc.info:eu-repo/semantics/openAccessExtracellular p-galactosidaseAdsorptionExpanded bedEnzyme recoveryExpanded-bed adsorption utilizing ion-exchange resin to purify extracellular β-galactosidaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf446862https://locus.ufv.br//bitstream/123456789/19616/1/artigo.pdf336e456a3de0014f5b452daf23b3bb21MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/19616/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg4321https://locus.ufv.br//bitstream/123456789/19616/3/artigo.pdf.jpgbf0c5b683149b6a1e11f1c026e07325cMD53123456789/196162018-05-16 23:01:03.204oai:locus.ufv.br:123456789/19616Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-05-17T02:01:03LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Expanded-bed adsorption utilizing ion-exchange resin to purify extracellular β-galactosidase
title Expanded-bed adsorption utilizing ion-exchange resin to purify extracellular β-galactosidase
spellingShingle Expanded-bed adsorption utilizing ion-exchange resin to purify extracellular β-galactosidase
Pereira, José Antonio Marques
Extracellular p-galactosidase
Adsorption
Expanded bed
Enzyme recovery
title_short Expanded-bed adsorption utilizing ion-exchange resin to purify extracellular β-galactosidase
title_full Expanded-bed adsorption utilizing ion-exchange resin to purify extracellular β-galactosidase
title_fullStr Expanded-bed adsorption utilizing ion-exchange resin to purify extracellular β-galactosidase
title_full_unstemmed Expanded-bed adsorption utilizing ion-exchange resin to purify extracellular β-galactosidase
title_sort Expanded-bed adsorption utilizing ion-exchange resin to purify extracellular β-galactosidase
author Pereira, José Antonio Marques
author_facet Pereira, José Antonio Marques
Rosa, Paulo De Tarso Vieira E.
Pastore, Glaucia Maria
Santana, Cesar Costapinto
author_role author
author2 Rosa, Paulo De Tarso Vieira E.
Pastore, Glaucia Maria
Santana, Cesar Costapinto
author2_role author
author
author
dc.contributor.author.fl_str_mv Pereira, José Antonio Marques
Rosa, Paulo De Tarso Vieira E.
Pastore, Glaucia Maria
Santana, Cesar Costapinto
dc.subject.pt-BR.fl_str_mv Extracellular p-galactosidase
Adsorption
Expanded bed
Enzyme recovery
topic Extracellular p-galactosidase
Adsorption
Expanded bed
Enzyme recovery
description The application of expanded-bed ion-exchange resins allows the elimination of intermediary particulate separation steps like filtration or centrifugation prior to adsorption steps in enzyme-purification processes from crude fermentation broths. This work is concerned with the experimental evaluation data of a process related to the adsorption of an extracellular p-galactosidase from the fungiScopulariopsis. The protein recovery in the ion-exchange resin Accell Plus QMA™ was accomplished using a continuous-monitoring method. The direct adsorption step was followed by a elution step with concentrated NaCl solutions aiming to improve the enzyme-specific activity. Experimental data for fixed and expanded bed were compared
publishDate 1998
dc.date.issued.fl_str_mv 1998-03
dc.date.accessioned.fl_str_mv 2018-05-16T13:55:59Z
dc.date.available.fl_str_mv 2018-05-16T13:55:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1007/BF02920188
http://www.locus.ufv.br/handle/123456789/19616
dc.identifier.issn.none.fl_str_mv 15590291
identifier_str_mv 15590291
url https://doi.org/10.1007/BF02920188
http://www.locus.ufv.br/handle/123456789/19616
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv v. 70, Issue 1, p. 779–787, March 1998
dc.rights.driver.fl_str_mv Humana Press Inc.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Humana Press Inc.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Applied Biochemistry and Biotechnology
publisher.none.fl_str_mv Applied Biochemistry and Biotechnology
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
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reponame_str LOCUS Repositório Institucional da UFV
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