Efeito de tinturas de café na osteoporose e no diabetes e do flavonóide hesperidina na osteoporose

• Autor(a) principal: Cardoso, Luciana Marques
• Data de Publicação: 2008
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2404
• topic: Flavonóides; Coffea arabica; Diabetes; Osteoporose; Flavonoids; Coffea arabica; Diabetes; Osteoporosis; CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR

Coffee consumption has been associated with a lower risk of type 2 diabetes. However, the specific compounds and mechanisms responsible for this effect are not clear. Three experiments were carried out to evaluate catuaí red coffee (Coffea arábica) tincture in rats with diabetes induced by alloxan administration (60mg/kg of corporal weight). The first experiment used cherry coffee hull, green coffee and cherry coffee tincture. In the second experiment the animals were treated with coffee dryleaf and coffee green leaf tinctures. The third experiment used the tincture of a commercial soluble coffee. All the tinctures tested in the 0.5 mL, 1.0 mL and 2.0 mL doses. After 30 days of treatment, samples of blood of the animals by dosages of the glucose serum, cholesterol and tryacylglicerols were collected. The cherry coffee hull (1.0 mL), green coffee (0.5 mL, 1.0 mL and 2.0 mL), cherry coffee (1.0 mL and 2.0 mL), grean leaf (0.5 mL, and 1.0 mL) and soluble coffee (1.0 mL and 2.0 mL) tinctures promoted a small increase, however significant, in the levels of cholesterol compared to the non-treated sick group. The dryleaf tincture (2.0 mL) was only one capable of reducing cholesterol concentrations significantly. In relation to the levels of glucose and tryacylglicerols, it was found that all the tinctures had significantly reduced these parameters, except the treatment with soluble coffee tincture (2.0 mL) did not result in a decrease the tryacylglicerols. The reduction percentages of the glucose and tryacylglicerols concentration varied from 20 to 49% and from 27 to 57%, respectively. The benefits of coffee tinctures used in this work in the glucose and tryacylglicerol levels overlap the possible negative effects with cholesterol, indicating that these tinctures can be promising for diabetes treatment. In relation to osteoporosis, the consumption of caffeine, a substance present in coffee, has been considered a risk factor. And hesperidin, the citrus flavonoid, has been evaluated as a powerful antiinflammatory agent,for its apparent antioxidant activity. Its antioxidant effect inhibits the superoxide formation, composites which are involved in the increase of osteoclast activity. Therefore, the hesperidina can be beneficial in the prevention of bone loss. The objective of the second part of this study was to evaluate the influence of the hesperidin flavonoid and coffee tinctures in rabbits with osteoporosis induced by the administration of glucocorticoid (7mg/kg of corporal weight). The animals were distributed in 6 groups: G1: ration + dexametasone (control with osteoporosis 1); G2: ration (control) G3: ration + dexametasone + 1mL of cherry coffee; G4: ration + dexametasone + 1 mL of cherry coffee hull; G5: ration + dexametasone (control with osteoporosis 2); G6: ration + dexametasone + 30 mg of hesperidin. Groups 1 to 4 had younger rabbits (48 days old). Groups 5 and 6 had 140 day olds. Due to the difference in age of the animals, they were probably in distinct periods of bone remodeling. Thus, the experiment was constituted with two non-treated sick groups, groups 1 and 5, with 48 and 140 days old, respectively. After 30 days of treatment, the samples of blood were collected from the animals to determine of the serum biochemists parameters (calcium, phosphorus, glucose, total fosfatase alkaline, urea, creatinine, total proteins, total cholesterol, tryacylglicerols, gamma GT and albumin) and of hematological parameters, such as: leukocytes, linphocytes, erythrocytes, mielocyte, granulocyte, hemoglobin, hematócrito, width corpuscular volume (MCV), red cell distibuition width (RDW), corpuscular hemoglobin width (MCH), corpuscular hemoglobin concentration (MCHC). In the youngest animal group, it was found that in the normal group the levels of urea, phosphorus, total fosfatase alkaline and albumin were statistically higher in the non-treated sick group (G1). The levels of glucose, tryacylglicerols and cholesterol were lower. In relation to the glucose, the group treated with cherry coffee had an increase in this parameter, but not significant. The cholesterol levels had been statistically lower in the group treated with cherry coffee and cherry coffee hull in relation to G1. The concentrations of tryacylglicerides had been statistically higher in G3 compared to G1. The animals treated with cherry coffee had significantly higher concentrations of phosphorus compared to G1. There were no significant differences in the following parameters of urea, creatinine, total proteins, calcium, total fosfatase alkaline, gamma GT and albumin when comparing the treatments to G1. The treatment with the hesperidin flavonoid significantly reduced the values of urea, glucose, tryacylglicerols and cholesterol in comparison to G5. In relation to the hematological parameters, it was found that in the normal group the total levels of leukocytes, linfphocyte, mielocyte, eritrocyte and hematocrit where statistically higher compared to the non-treated sick group (G1). The concentrations of leukocytes, mielocyte, granulocyte and MCV had been significantly higher in the treatment with cherry coffee, compared with G1. Cherry coffee hull treatment a significant reduction in the hemoglobin and hematocrit was observed, compared to G1. Significant differences in the parameters there were in (RDW), MCH and MCHC when comparing the treated and non-treated sick group (G1). In the older animal groups, no significant differences in the parameters of creatinine, total proteins, calcium, phosphorus, cholesterol, glucose, total fosfatase alkaline, gamma GT and albumin were observed among the treatments and the non-treated sick group (G5). No statistical difference was observed in the hematological parameters between the treatment with hesperidin and the group that received only dexametasone (G5).