High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1007/s11103-017-0676-7 http://www.locus.ufv.br/handle/123456789/18638 |
Resumo: | Coffee rust disease, caused by the fungus Hemileia vastatrix, is one of the major diseases in coffee throughout the world. The use of resistant cultivars is considered to be the most effective control strategy for this disease. To identify candidate genes related to different mechanism defense in coffee, we present a time-course comparative gene expression profile of Caturra (susceptible) and Híbrido de Timor (HdT, resistant) in response to H. vastatrix race XXXIII infection. The main objectives were to obtain a global overview of transcriptome in both interaction, compatible and incompatible, and, specially, analyze up-regulated HdT specific genes with inducible resistant and defense signaling pathways. Using both Coffea canephora as a reference genome and de novo assembly, we obtained 43,159 transcripts. At early infection events (12 and 24 h after infection), HdT responded to the attack of H. vastatrix with a larger number of up-regulated genes than Caturra, which was related to prehaustorial resistance. The genes found in HdT at early hours were involved in receptor-like kinases, response ion fluxes, production of reactive oxygen species, protein phosphorylation, ethylene biosynthesis and callose deposition. We selected 13 up-regulated HdT-exclusive genes to validate by real-time qPCR, which most of them confirmed their higher expression in HdT than in Caturra at early stage of infection. These genes have the potential to assist the development of new coffee rust control strategies. Collectively, our results provide understanding of expression profiles in coffee—H. vastatrix interaction over a time course in susceptible and resistant coffee plants. |
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Florez, Juan CarlosMofatto, Luciana SoutoFreitas-Lopes, Rejane do LivramentoFerreira, Sávio SiqueiraZambolim, Eunize MacielCarazzolle, Marcelo FalsarellaZambolim, LaércioCaixeta, Eveline Teixeira2018-04-04T11:04:19Z2018-04-04T11:04:19Z2017-11-0115735028https://doi.org/10.1007/s11103-017-0676-7http://www.locus.ufv.br/handle/123456789/18638Coffee rust disease, caused by the fungus Hemileia vastatrix, is one of the major diseases in coffee throughout the world. The use of resistant cultivars is considered to be the most effective control strategy for this disease. To identify candidate genes related to different mechanism defense in coffee, we present a time-course comparative gene expression profile of Caturra (susceptible) and Híbrido de Timor (HdT, resistant) in response to H. vastatrix race XXXIII infection. The main objectives were to obtain a global overview of transcriptome in both interaction, compatible and incompatible, and, specially, analyze up-regulated HdT specific genes with inducible resistant and defense signaling pathways. Using both Coffea canephora as a reference genome and de novo assembly, we obtained 43,159 transcripts. At early infection events (12 and 24 h after infection), HdT responded to the attack of H. vastatrix with a larger number of up-regulated genes than Caturra, which was related to prehaustorial resistance. The genes found in HdT at early hours were involved in receptor-like kinases, response ion fluxes, production of reactive oxygen species, protein phosphorylation, ethylene biosynthesis and callose deposition. We selected 13 up-regulated HdT-exclusive genes to validate by real-time qPCR, which most of them confirmed their higher expression in HdT than in Caturra at early stage of infection. These genes have the potential to assist the development of new coffee rust control strategies. Collectively, our results provide understanding of expression profiles in coffee—H. vastatrix interaction over a time course in susceptible and resistant coffee plants.engPlant Molecular Biologyv. 95, Issue 6, p. 607–623, December 2017Springer Science+Business Media B.V.info:eu-repo/semantics/openAccessCoffee rustTranscriptomeBiotrophic interactionDisease resistanceHigh throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf3011854https://locus.ufv.br//bitstream/123456789/18638/1/artigo.pdf4eee987f2f8eb3d71dffd122e8729a6dMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/18638/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg4656https://locus.ufv.br//bitstream/123456789/18638/3/artigo.pdf.jpg569e01deb4a8b90d6e41df2231f918faMD53123456789/186382018-04-04 23:00:55.618oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-04-05T02:00:55LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection |
title |
High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection |
spellingShingle |
High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection Florez, Juan Carlos Coffee rust Transcriptome Biotrophic interaction Disease resistance |
title_short |
High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection |
title_full |
High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection |
title_fullStr |
High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection |
title_full_unstemmed |
High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection |
title_sort |
High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection |
author |
Florez, Juan Carlos |
author_facet |
Florez, Juan Carlos Mofatto, Luciana Souto Freitas-Lopes, Rejane do Livramento Ferreira, Sávio Siqueira Zambolim, Eunize Maciel Carazzolle, Marcelo Falsarella Zambolim, Laércio Caixeta, Eveline Teixeira |
author_role |
author |
author2 |
Mofatto, Luciana Souto Freitas-Lopes, Rejane do Livramento Ferreira, Sávio Siqueira Zambolim, Eunize Maciel Carazzolle, Marcelo Falsarella Zambolim, Laércio Caixeta, Eveline Teixeira |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Florez, Juan Carlos Mofatto, Luciana Souto Freitas-Lopes, Rejane do Livramento Ferreira, Sávio Siqueira Zambolim, Eunize Maciel Carazzolle, Marcelo Falsarella Zambolim, Laércio Caixeta, Eveline Teixeira |
dc.subject.pt-BR.fl_str_mv |
Coffee rust Transcriptome Biotrophic interaction Disease resistance |
topic |
Coffee rust Transcriptome Biotrophic interaction Disease resistance |
description |
Coffee rust disease, caused by the fungus Hemileia vastatrix, is one of the major diseases in coffee throughout the world. The use of resistant cultivars is considered to be the most effective control strategy for this disease. To identify candidate genes related to different mechanism defense in coffee, we present a time-course comparative gene expression profile of Caturra (susceptible) and Híbrido de Timor (HdT, resistant) in response to H. vastatrix race XXXIII infection. The main objectives were to obtain a global overview of transcriptome in both interaction, compatible and incompatible, and, specially, analyze up-regulated HdT specific genes with inducible resistant and defense signaling pathways. Using both Coffea canephora as a reference genome and de novo assembly, we obtained 43,159 transcripts. At early infection events (12 and 24 h after infection), HdT responded to the attack of H. vastatrix with a larger number of up-regulated genes than Caturra, which was related to prehaustorial resistance. The genes found in HdT at early hours were involved in receptor-like kinases, response ion fluxes, production of reactive oxygen species, protein phosphorylation, ethylene biosynthesis and callose deposition. We selected 13 up-regulated HdT-exclusive genes to validate by real-time qPCR, which most of them confirmed their higher expression in HdT than in Caturra at early stage of infection. These genes have the potential to assist the development of new coffee rust control strategies. Collectively, our results provide understanding of expression profiles in coffee—H. vastatrix interaction over a time course in susceptible and resistant coffee plants. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-11-01 |
dc.date.accessioned.fl_str_mv |
2018-04-04T11:04:19Z |
dc.date.available.fl_str_mv |
2018-04-04T11:04:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1007/s11103-017-0676-7 http://www.locus.ufv.br/handle/123456789/18638 |
dc.identifier.issn.none.fl_str_mv |
15735028 |
identifier_str_mv |
15735028 |
url |
https://doi.org/10.1007/s11103-017-0676-7 http://www.locus.ufv.br/handle/123456789/18638 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v. 95, Issue 6, p. 607–623, December 2017 |
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Springer Science+Business Media B.V. info:eu-repo/semantics/openAccess |
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Springer Science+Business Media B.V. |
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openAccess |
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Plant Molecular Biology |
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Plant Molecular Biology |
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