Separomics applied to the proteomics and peptidomics of low-abundance proteins: choice of methods and challenges - a review

• Autor(a) principal: Baracat-Pereira, Maria Cristina
• Data de Publicação: 2011
• Outros Autores: Barbosa, Meire de Oliveira, Magalhães Júnior, Marcos Jorge, Carrijo, Lanna Clicia, Games, Patrícia Dias, Almeida, Hebréia Oliveira, Sena Netto, José Fabiano, Pereira, Matheus Rodrigues, Barros, Everaldo Gonçalves de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://dx.doi.org/10.1590/S1415-47572012000200009; http://www.locus.ufv.br/handle/123456789/12551
• topic: Sample preparation; Complex protein extract; Subproteomes; Low-abundance proteins; Cell wall proteins

The enrichment and isolation of proteins are considered limiting steps in proteomic studies. Identification of proteins whose expression is transient, those that are of low-abundance, and of natural peptides not described in databases, is still a great challenge. Plant extracts are in general complex, and contaminants interfere with the identification of proteins involved in important physiological processes, such as plant defense against pathogens. This review discusses the challenges and strategies of separomics applied to the identification of low-abundance proteins and peptides in plants, especially in plants challenged by pathogens. Separomics is described as a group of methodological strategies for the separation of protein molecules for proteomics. Several tools have been used to remove highly abundant proteins from samples and also non-protein contaminants. The use of chromatographic techniques, the partition of the proteome into subproteomes, and an effort to isolate proteins in their native form have allowed the isolation and identification of rare proteins involved in different processes.