Alterações fisiológicas e bioquímicas em sementes de repolho (Brassica oleracea var. Capitata) osmocondicionadas

• Autor(a) principal: Martinez, Paola Andrea Hormaza
• Data de Publicação: 2013
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/4623
• topic: Osmocondicionamento; Envelhecimento acelerado; Brassica oleracea; Carboidratos; Lipídeos e proteínas; Osmopriming; Accelerated aging; Brassica oleracea; Carbohydrates; Lipids and proteins; CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA

This work aimed to evaluated the biochemical and physiological changes resulting from priming of cabbage seeds with different vigor levels, obtained after to exposure of seeds to different times of accelerated aging. The seeds were subjected to priming in a polyethylene glicol aerated solution (PEG) 6000 in concentration of 284 g L-1, corresponding to the osmotic potential of -1.0 MPa, for six days. After priming the seeds were dried until reaching the initial water content. The control consisted of unprimed seeds. The seeds of each treatment were tested for germination, first count germination, seedling emergence, controlled deterioration test, speed germination index, germination rate index in substrate, germination speed, radicle length and total dry matter. Besides, were performed the germination curves for each level of force. Also were performed, analysis for lipid, carbohydrate, protein and starch content. The experimental design was completely randomized with four replications in a 4x2 factorial (aged seeds for 24, 33 and 42 hours and not aged seeds) with and without priming. The data were submeted to Analysis of variance and differences between means were performed using Tukey and F tests, respectively, both at p <0.05. Were observed beneficial priming effects effectively minimizing the damage caused by artificial againg longer on seeds (33 and 48 hours). Superior results were found in tests of vigor, seedling dry weight and reserve compounds when compared to the control unconditioned. In seeds with lower physiological unprimed the imbibition time for root protrusion was greater. Lower times of germination were recorded for seeds primed, which showed higher protein content and lower content of total soluble and reducing sugars, high germination percentage and vigor when compared to unpriming seeds. Therefore, it can be concluded that priming may be an alternative to improve the physiological quality of less vigorous seeds subjected to stress from high temperature and humidity.