Fungos endofíticos de Phaseolus vulgaris exibem atividade antimicrobiana e potencial para controle de fitopatógenos

Detalhes bibliográficos
Autor(a) principal: Santos, Taides Tavares dos
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/5371
Resumo: Endophytic fungi are those living at least a part of their life cycle within plant tissue, apparently without causing any damage to their hosts. These microorganisms have been studied for their capacity to produce secondary metabolites of biotechnological interest, and also for their ability for biological control of phytopathogens. Endophytic fungi have been isolated from a wide variety of plant species, including crops such as common bean (Phaseolus vulgaris L.). Fungi from the genus Diaporthe were abundant in the endophytic community of this legume. The objectives of this study were to evaluate the antimicrobial activity and the potential use of endophytic fungi of P. vulgaris in the control of phytopathogens of bean, and to analyze phylogenetic relationships and genetic variability of endophytic fungi of the genus Diaporthe isolated from P. vulgaris. Dual culture assays were performed between ninety endophytic fungi and four phytopathogenic fungi (Colletotrichum lindemuthianum, Fusarium oxysporum, Rhizoctonia solani and Sclerotinia sclerotiorum) to assess for the ability of endophytic fungi inhibit the growth of phytopathogens. The isolates that were able to inhibit the four phytopathogens tested were selected for cultivation and obtention of metabolites crude extracts. These extracts were evaluated for antibacterial and antifungal activity. The sequences of the ITS region of rDNA of the isolates of the genus Diaporthe from common bean, along with the partial sequences of the genes encoding translation elongation factor 1-α, β-tubulin and calmodulin were used for the study of the phylogenetic relationships of these isolates. Molecular markers based on sequences of retrotransposons, IRAP (Inter-Retrotransposon Amplified Polymorphism) and REMAP (Retrotransposon-Microsatellite Amplified Polymorphism), were used for the analysis of genetic variability. It was found that endophytic fungi of common bean are able to inhibit the in vitro growth of phytopathogenic fungi and that metabolites crude extracts of endophytic exhibit significant antimicrobial activity especially regarding the inhibitory activity against Gram-positive bacteria (Listeria monocytogenes, Bacillus cereus, B. subtilis and Staphylococcus aureus). Diaporthe sp., D. infecunda, D. melonis and D. phaseolorum are members of the endophytic fungal community in the common bean as verified by multilocus phylogenetic analysis. Cluster analysis, conducted with pooled data from IRAP and REMAP markers, was consistent regarding what was observed in multilocus phylogeny and revealed the existence of high genetic variability, particularly among isolates of D. infecunda. It was concluded that the metabolites crude extracts of endophytic fungi of common bean exhibit promising antimicrobial activity; that endophytic fungi used in this study have the potential to control phytopathogens that affect the bean crop; the multilocus phylogenetic approach was more effective than individual analysis of ITS sequences in the study of phylogenetic relationships of endophytic fungi of the genus Diaporthe from common bean and that IRAP and REMAP markers can be employed in the study of genetic variability of this genus of fungi.
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spelling Santos, Taides Tavares doshttp://lattes.cnpq.br/0619959327505427Pereira, Olinto Liparinihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767879D4Araujo, Elza Fernandes dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783675E2Queiroz, Marisa Vieira dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785812Z5Ramos, Humberto Josué de Oliveirahttp://lattes.cnpq.br/40374529200801742015-03-26T13:52:00Z2014-11-282015-03-26T13:52:00Z2014-02-20SANTOS, Taides Tavares dos. Endophytic fungi of Phaseolus vulgaris exhibit antimicrobial activity and potential for control of phytopathogens. 2014. 97 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2014.http://locus.ufv.br/handle/123456789/5371Endophytic fungi are those living at least a part of their life cycle within plant tissue, apparently without causing any damage to their hosts. These microorganisms have been studied for their capacity to produce secondary metabolites of biotechnological interest, and also for their ability for biological control of phytopathogens. Endophytic fungi have been isolated from a wide variety of plant species, including crops such as common bean (Phaseolus vulgaris L.). Fungi from the genus Diaporthe were abundant in the endophytic community of this legume. The objectives of this study were to evaluate the antimicrobial activity and the potential use of endophytic fungi of P. vulgaris in the control of phytopathogens of bean, and to analyze phylogenetic relationships and genetic variability of endophytic fungi of the genus Diaporthe isolated from P. vulgaris. Dual culture assays were performed between ninety endophytic fungi and four phytopathogenic fungi (Colletotrichum lindemuthianum, Fusarium oxysporum, Rhizoctonia solani and Sclerotinia sclerotiorum) to assess for the ability of endophytic fungi inhibit the growth of phytopathogens. The isolates that were able to inhibit the four phytopathogens tested were selected for cultivation and obtention of metabolites crude extracts. These extracts were evaluated for antibacterial and antifungal activity. The sequences of the ITS region of rDNA of the isolates of the genus Diaporthe from common bean, along with the partial sequences of the genes encoding translation elongation factor 1-α, β-tubulin and calmodulin were used for the study of the phylogenetic relationships of these isolates. Molecular markers based on sequences of retrotransposons, IRAP (Inter-Retrotransposon Amplified Polymorphism) and REMAP (Retrotransposon-Microsatellite Amplified Polymorphism), were used for the analysis of genetic variability. It was found that endophytic fungi of common bean are able to inhibit the in vitro growth of phytopathogenic fungi and that metabolites crude extracts of endophytic exhibit significant antimicrobial activity especially regarding the inhibitory activity against Gram-positive bacteria (Listeria monocytogenes, Bacillus cereus, B. subtilis and Staphylococcus aureus). Diaporthe sp., D. infecunda, D. melonis and D. phaseolorum are members of the endophytic fungal community in the common bean as verified by multilocus phylogenetic analysis. Cluster analysis, conducted with pooled data from IRAP and REMAP markers, was consistent regarding what was observed in multilocus phylogeny and revealed the existence of high genetic variability, particularly among isolates of D. infecunda. It was concluded that the metabolites crude extracts of endophytic fungi of common bean exhibit promising antimicrobial activity; that endophytic fungi used in this study have the potential to control phytopathogens that affect the bean crop; the multilocus phylogenetic approach was more effective than individual analysis of ITS sequences in the study of phylogenetic relationships of endophytic fungi of the genus Diaporthe from common bean and that IRAP and REMAP markers can be employed in the study of genetic variability of this genus of fungi.Fungos endofíticos são aqueles que vivem, em pelo menos uma parte de seu ciclo de vida, no interior de tecidos vegetais, sem causar aparentemente qualquer dano a seus hospedeiros. Esses micro-organismos têm sido estudados em relação à capacidade de produzirem metabólitos secundários de interesse biotecnológico e quanto ao potencial de controle biológico de fitopatógenos. Fungos endofíticos já foram isolados de uma ampla variedade de espécies vegetais, incluindo culturas agrícolas como o feijoeiro comum (Phaseolus vulgaris L.). O gênero Diaporthe foi um dos mais abundantes na comunidade endofítica dessa leguminosa. Os objetivos do presente estudo foram avaliar a atividade antimicrobiana e o potencial de uso de fungos endofíticos de P. vulgaris no controle de fitopatógenos da cultura do feijoeiro e analisar as relações filogenéticas e a variabilidade genética de fungos endofíticos do gênero Diaporthe isolados de P. vulgaris. Ensaios de cultura dupla entre 90 isolados endofíticos e quatro fungos fitopatogênicos (Colletotrichum lindemuthianum, Fusarium oxysporum, Rhizoctonia solani e Sclerotinia sclerotiorum) foram realizados para verificar a capacidade dos isolados endofíticos inibirem o crescimento dos fitopatógenos. Os isolados que foram capazes de inibir os quatro fitopatógenos testados foram selecionados para cultivo e obtenção de extratos brutos de metabólitos. Esses extratos foram avaliados quanto à atividade antibacteriana e antifúngica. As sequências da região ITS do rDNA dos isolados do gênero Diaporthe do feijoeiro, juntamente com as sequências parciais dos genes codificam o fator de elongação da tradução 1-α, β-tubulina e calmodulina, foram utilizadas para o estudo das relações filogenéticas desses isolados. Marcadores moleculares baseados em sequências de retrotransposons, IRAP (Inter-Retrotransposon Amplified Polymorphism) e REMAP (Retrotransposon-Microsatellite Amplified Polymorphism), foram utilizados para a análise de variabilidade genética. Foi constatado que os fungos endofíticos do feijoeiro comum são capazes de inibir o crescimento in vitro de fungos fitopatogênicos e, que extratos brutos de metabólitos de isolados endofíticos exibem atividade antimicrobiana significativa, principalmente em relação à atividade inibitória sobre bactérias Gram-positivas (Listeria monocytogenes, Bacillus cereus, B. subtilis e Staphylococcus aureus). Por meio de análise filogenética multilocus, verificou-se que Diaporthe sp., D. infecunda, D. melonis e D. phaseolorum são integrantes da comunidade fúngica endofítica do feijoeiro comum. A análise de agrupamento, realizada com os dados dos marcadores IRAP e REMAP em conjunto, foi coerente em relação ao que foi verificado na filogenia multilocus e, revelou a existência de grande variabilidade genética, sobretudo entre os isolados de D. infecunda. Concluiu-se que extratos brutos de metabólitos de fungos endofíticos do feijoeiro exibem atividade antimicrobiana promissora; que fungos endofíticos utilizados neste estudo apresentam potencial para controle de fitopatógenos que acometem a cultura do feijoeiro; que a abordagem filogenética multilocus foi mais efetiva que análise individual de sequências de ITS no estudo das relações filogenéticas de fungos endofíticos do gênero Diaporthe do feijoeiro e que marcadores IRAP e REMAP podem ser empregados no estudo de variabilidade genética de fungos desse gênero.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em Microbiologia AgrícolaUFVBRAssociações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesseFungos endofíticosControle de fitopatógenosVariabilidade genéticaEndophytic fungiControl plant pathogensgenetic variabilityCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::MICROBIOLOGIA AGRICOLAFungos endofíticos de Phaseolus vulgaris exibem atividade antimicrobiana e potencial para controle de fitopatógenosEndophytic fungi of Phaseolus vulgaris exhibit antimicrobial activity and potential for control of phytopathogensinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf1252953https://locus.ufv.br//bitstream/123456789/5371/1/texto%20completo.pdf5c4901cfbd21633e7c02d3de0ad4790aMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain182700https://locus.ufv.br//bitstream/123456789/5371/2/texto%20completo.pdf.txt2a0d9735ceb36c4bdf16b34877230311MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3396https://locus.ufv.br//bitstream/123456789/5371/3/texto%20completo.pdf.jpg1d78c835f22d720e2f8709b1f5da8247MD53123456789/53712016-04-10 23:19:14.224oai:locus.ufv.br:123456789/5371Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:19:14LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Fungos endofíticos de Phaseolus vulgaris exibem atividade antimicrobiana e potencial para controle de fitopatógenos
dc.title.alternative.eng.fl_str_mv Endophytic fungi of Phaseolus vulgaris exhibit antimicrobial activity and potential for control of phytopathogens
title Fungos endofíticos de Phaseolus vulgaris exibem atividade antimicrobiana e potencial para controle de fitopatógenos
spellingShingle Fungos endofíticos de Phaseolus vulgaris exibem atividade antimicrobiana e potencial para controle de fitopatógenos
Santos, Taides Tavares dos
Fungos endofíticos
Controle de fitopatógenos
Variabilidade genética
Endophytic fungi
Control plant pathogens
genetic variability
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::MICROBIOLOGIA AGRICOLA
title_short Fungos endofíticos de Phaseolus vulgaris exibem atividade antimicrobiana e potencial para controle de fitopatógenos
title_full Fungos endofíticos de Phaseolus vulgaris exibem atividade antimicrobiana e potencial para controle de fitopatógenos
title_fullStr Fungos endofíticos de Phaseolus vulgaris exibem atividade antimicrobiana e potencial para controle de fitopatógenos
title_full_unstemmed Fungos endofíticos de Phaseolus vulgaris exibem atividade antimicrobiana e potencial para controle de fitopatógenos
title_sort Fungos endofíticos de Phaseolus vulgaris exibem atividade antimicrobiana e potencial para controle de fitopatógenos
author Santos, Taides Tavares dos
author_facet Santos, Taides Tavares dos
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/0619959327505427
dc.contributor.author.fl_str_mv Santos, Taides Tavares dos
dc.contributor.advisor-co1.fl_str_mv Pereira, Olinto Liparini
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767879D4
dc.contributor.advisor-co2.fl_str_mv Araujo, Elza Fernandes de
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783675E2
dc.contributor.advisor1.fl_str_mv Queiroz, Marisa Vieira de
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785812Z5
dc.contributor.referee1.fl_str_mv Ramos, Humberto Josué de Oliveira
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/4037452920080174
contributor_str_mv Pereira, Olinto Liparini
Araujo, Elza Fernandes de
Queiroz, Marisa Vieira de
Ramos, Humberto Josué de Oliveira
dc.subject.por.fl_str_mv Fungos endofíticos
Controle de fitopatógenos
Variabilidade genética
topic Fungos endofíticos
Controle de fitopatógenos
Variabilidade genética
Endophytic fungi
Control plant pathogens
genetic variability
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::MICROBIOLOGIA AGRICOLA
dc.subject.eng.fl_str_mv Endophytic fungi
Control plant pathogens
genetic variability
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::MICROBIOLOGIA AGRICOLA
description Endophytic fungi are those living at least a part of their life cycle within plant tissue, apparently without causing any damage to their hosts. These microorganisms have been studied for their capacity to produce secondary metabolites of biotechnological interest, and also for their ability for biological control of phytopathogens. Endophytic fungi have been isolated from a wide variety of plant species, including crops such as common bean (Phaseolus vulgaris L.). Fungi from the genus Diaporthe were abundant in the endophytic community of this legume. The objectives of this study were to evaluate the antimicrobial activity and the potential use of endophytic fungi of P. vulgaris in the control of phytopathogens of bean, and to analyze phylogenetic relationships and genetic variability of endophytic fungi of the genus Diaporthe isolated from P. vulgaris. Dual culture assays were performed between ninety endophytic fungi and four phytopathogenic fungi (Colletotrichum lindemuthianum, Fusarium oxysporum, Rhizoctonia solani and Sclerotinia sclerotiorum) to assess for the ability of endophytic fungi inhibit the growth of phytopathogens. The isolates that were able to inhibit the four phytopathogens tested were selected for cultivation and obtention of metabolites crude extracts. These extracts were evaluated for antibacterial and antifungal activity. The sequences of the ITS region of rDNA of the isolates of the genus Diaporthe from common bean, along with the partial sequences of the genes encoding translation elongation factor 1-α, β-tubulin and calmodulin were used for the study of the phylogenetic relationships of these isolates. Molecular markers based on sequences of retrotransposons, IRAP (Inter-Retrotransposon Amplified Polymorphism) and REMAP (Retrotransposon-Microsatellite Amplified Polymorphism), were used for the analysis of genetic variability. It was found that endophytic fungi of common bean are able to inhibit the in vitro growth of phytopathogenic fungi and that metabolites crude extracts of endophytic exhibit significant antimicrobial activity especially regarding the inhibitory activity against Gram-positive bacteria (Listeria monocytogenes, Bacillus cereus, B. subtilis and Staphylococcus aureus). Diaporthe sp., D. infecunda, D. melonis and D. phaseolorum are members of the endophytic fungal community in the common bean as verified by multilocus phylogenetic analysis. Cluster analysis, conducted with pooled data from IRAP and REMAP markers, was consistent regarding what was observed in multilocus phylogeny and revealed the existence of high genetic variability, particularly among isolates of D. infecunda. It was concluded that the metabolites crude extracts of endophytic fungi of common bean exhibit promising antimicrobial activity; that endophytic fungi used in this study have the potential to control phytopathogens that affect the bean crop; the multilocus phylogenetic approach was more effective than individual analysis of ITS sequences in the study of phylogenetic relationships of endophytic fungi of the genus Diaporthe from common bean and that IRAP and REMAP markers can be employed in the study of genetic variability of this genus of fungi.
publishDate 2014
dc.date.available.fl_str_mv 2014-11-28
2015-03-26T13:52:00Z
dc.date.issued.fl_str_mv 2014-02-20
dc.date.accessioned.fl_str_mv 2015-03-26T13:52:00Z
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dc.identifier.citation.fl_str_mv SANTOS, Taides Tavares dos. Endophytic fungi of Phaseolus vulgaris exhibit antimicrobial activity and potential for control of phytopathogens. 2014. 97 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2014.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/5371
identifier_str_mv SANTOS, Taides Tavares dos. Endophytic fungi of Phaseolus vulgaris exhibit antimicrobial activity and potential for control of phytopathogens. 2014. 97 f. Dissertação (Mestrado em Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse) - Universidade Federal de Viçosa, Viçosa, 2014.
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dc.publisher.department.fl_str_mv Associações micorrízicas; Bactérias láticas e probióticos; Biologia molecular de fungos de interesse
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