Citotoxicidade de dois cimentos à base de silicato de cálcio
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UNIGRANRIO |
Texto Completo: | http://localhost:8080/tede/handle/tede/231 |
Resumo: | The aim of the present study was to evaluate MTA White and TheraCal LC Cytotoxicity. Control group used was treated with convencional cellular medium. The Cytotocity was determined by counting viable cells using the MTT colorimetric Assay. The extract of the materials was used undiluted and an experimental contact Time for 24 hours. Data from the MTT assay was performed using SPSS version 15.0 statistical software (SPSS, INC, Chicago,IL, USA). Data were tested for Normal distribution by the Kolmogorov-Smirnov test, and group comparisons were Analysed by one-way analysis of variance (ANOVA) followed by a Bonferroni post- test. The results showed statistically significant differences between the materials Testd ( P> 0.05 ) and between the materials and the control group ( P> 0.5 ). Its is Concluded that, within the limitations of the study, ther is difference between a MTA cytotoxicity where when compared to the TheraCal LC. |
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Mendonça, Thais AccorsiMoreira, Edson Jorge LimaSilva, Emmanoel João Nogueira Leal daBatista, Marcelo Mangelli DecnopVieira, Victor Talarico LealEstellita, Raffaella Sacchetti2018-07-18T15:06:21Z2017-03-08Estellita, Raffaella Sacchetti. Citotoxicidade de dois cimentos à base de silicato de cálcio. 2017. 35 f. Dissertação (Programa de Pós-Graduação em Odontologia) - Universidade do Grande Rio, Duque de Caxias.http://localhost:8080/tede/handle/tede/231The aim of the present study was to evaluate MTA White and TheraCal LC Cytotoxicity. Control group used was treated with convencional cellular medium. The Cytotocity was determined by counting viable cells using the MTT colorimetric Assay. The extract of the materials was used undiluted and an experimental contact Time for 24 hours. Data from the MTT assay was performed using SPSS version 15.0 statistical software (SPSS, INC, Chicago,IL, USA). Data were tested for Normal distribution by the Kolmogorov-Smirnov test, and group comparisons were Analysed by one-way analysis of variance (ANOVA) followed by a Bonferroni post- test. The results showed statistically significant differences between the materials Testd ( P> 0.05 ) and between the materials and the control group ( P> 0.5 ). Its is Concluded that, within the limitations of the study, ther is difference between a MTA cytotoxicity where when compared to the TheraCal LC.O objetivo deste estudo foi avaliar os efeitos de exposição de dois cimentos à base De silicato de cálcio (MTA/Angelus e Theracal LC /Bisco) sobre osteoblastos Humanos imortalizados (linhagem São-2), quando comparado ao grupo controle, Onde as células não foram expostas a nenhum extrato, somente ao meio Convencional de cultura celular. A citotoxicidade dos cimentos foi determinada pela Contagem de células viáveis utilizando-se o ensaio colorimétrico MTT. Utilizou-se o Extrato dos cimentos de forma não diluída e um tempo experimental de contato Com as células por 24 horas> os resultados foram analisados estatisticamente Através do software SPSS (versão 15.0,SSPSS, Inc, Chicago, IL,USA) e testados Para uma distribuição normal através do teste de Kolmogorov- Smirnov, e para as Comparações entre grupos utilizou-se o ensaio ANOV seguindo de Bonferroni post- Test. O cimento MTA não apresentou citotoxicidade quando comparado ao grupo Controle ( P> 0.05 ), já o cimento Theracal foi citotóxico, demonstrando diferença Estatisticamente significante quando comparado ao MTA e ao grupo controle (P>0.05). Conclui-se que, dentro das limitações do estudo, que o MTA branco Apresentou menor citotoxidade quando comparado ao TheraCal LC.Submitted by Patricia Vieira Silva (patricia.silva@unigranrio.com.br) on 2018-07-18T15:06:21Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) RAFFAELLA SACCHETTI ESTELLITA 2017.pdf: 1890548 bytes, checksum: 66eab8a25a5104110143d3e01d452c51 (MD5)Made available in DSpace on 2018-07-18T15:06:21Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) RAFFAELLA SACCHETTI ESTELLITA 2017.pdf: 1890548 bytes, checksum: 66eab8a25a5104110143d3e01d452c51 (MD5) Previous issue date: 2017-03-08application/pdfporUniversidade do Grande RioPrograma de Pós-Graduação em OdontologiaUNIGRANRIOBrasilUnigranrio::Odontologiahttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessOdontologiaEndodontiaCitotoxicidadeMateriais dentáriosCimentos dentáriosOdontologiaEndodontiaCitotoxicidade de dois cimentos à base de silicato de cálcioinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisreponame:Biblioteca Digital de Teses e Dissertações da UNIGRANRIOinstname:Universidade do Grande Rio (UNIGRANRIO)instacron:UNIGRANRIOLICENSElicense.txtlicense.txttext/plain; charset=utf-81982http://localhost:8080/tede/bitstream/tede/231/1/license.txt4a50535e8405f611398e6e2d408dbd1bMD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://localhost:8080/tede/bitstream/tede/231/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://localhost:8080/tede/bitstream/tede/231/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://localhost:8080/tede/bitstream/tede/231/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54ORIGINALRAFFAELLA SACCHETTI ESTELLITA 2017.pdfRAFFAELLA SACCHETTI ESTELLITA 2017.pdfapplication/pdf1890548http://localhost:8080/tede/bitstream/tede/231/5/RAFFAELLA+SACCHETTI+ESTELLITA+2017.pdf66eab8a25a5104110143d3e01d452c51MD55tede/2312018-07-18 12:06:21.952oai:localhost:tede/231CkxJQ0VOw4dBIERFIERJU1RSSUJVScOHw4NPIE7Dg08tRVhDTFVTSVZBCgpDb20gYSBhcHJlc2VudGHDp8OjbyBkZXN0YSBsaWNlbsOnYSwgdm9jw6ogKG8gYXV0b3IgKGVzKSBvdSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGRlIGF1dG9yKSBjb25jZWRlIMOgIFVuaXZlcnNpZGFkZSAKVU5JR1JBTlJJTyBvIGRpcmVpdG8gbsOjby1leGNsdXNpdm8gZGUgcmVwcm9kdXppciwgIHRyYWR1emlyIChjb25mb3JtZSBkZWZpbmlkbyBhYmFpeG8pLCBlL291IApkaXN0cmlidWlyIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0csO0bmljbyBlIAplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFVOSUdSQU5SSU8gcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250ZcO6ZG8sIHRyYW5zcG9yIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAKcGFyYSBxdWFscXVlciBtZWlvIG91IGZvcm1hdG8gcGFyYSBmaW5zIGRlIHByZXNlcnZhw6fDo28uCgpWb2PDqiB0YW1iw6ltIGNvbmNvcmRhIHF1ZSBhIFVOSUdSQU5SSU8gcG9kZSBtYW50ZXIgbWFpcyBkZSB1bWEgY8OzcGlhIGRhIHN1YSB0ZXNlIG91IApkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcyAKbmVzdGEgbGljZW7Dp2EuIFZvY8OqIHRhbWLDqW0gZGVjbGFyYSBxdWUgbyBkZXDDs3NpdG8gZGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBuw6NvLCBxdWUgc2VqYSBkZSBzZXUgCmNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3XDqW0uCgpDYXNvIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jw6ogbsOjbyBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2PDqiAKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc8OjbyBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyIMOgIFVOSUdSQU5SSU8gCm9zIGRpcmVpdG9zIGFwcmVzZW50YWRvcyBuZXN0YSBsaWNlbsOnYSwgZSBxdWUgZXNzZSBtYXRlcmlhbCBkZSBwcm9wcmllZGFkZSBkZSB0ZXJjZWlyb3MgZXN0w6EgY2xhcmFtZW50ZSAKaWRlbnRpZmljYWRvIGUgcmVjb25oZWNpZG8gbm8gdGV4dG8gb3Ugbm8gY29udGXDumRvIGRhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBvcmEgZGVwb3NpdGFkYS4KCkNBU08gQSBURVNFIE9VIERJU1NFUlRBw4fDg08gT1JBIERFUE9TSVRBREEgVEVOSEEgU0lETyBSRVNVTFRBRE8gREUgVU0gUEFUUk9Dw41OSU8gT1UgCkFQT0lPIERFIFVNQSBBR8OKTkNJQSBERSBGT01FTlRPIE9VIE9VVFJPIE9SR0FOSVNNTyBRVUUgTsODTyBTRUpBIEEgVU5JR1JBTlJJTywgClZPQ8OKIERFQ0xBUkEgUVVFIFJFU1BFSVRPVSBUT0RPUyBFIFFVQUlTUVVFUiBESVJFSVRPUyBERSBSRVZJU8ODTyBDT01PIApUQU1Cw4lNIEFTIERFTUFJUyBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUE9SIENPTlRSQVRPIE9VIEFDT1JETy4KCkEgVU5JR1JBTlJJTyBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lIChzKSBvdSBvKHMpIG5vbWUocykgZG8ocykgCmRldGVudG9yKGVzKSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvLCBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIGFsw6ltIGRhcXVlbGFzIApjb25jZWRpZGFzIHBvciBlc3RhIGxpY2Vuw6dhLgo=Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unigranrio.edu.br/PUBhttp://tede.unigranrio.edu.br/oai/requestrepositorio@instituicao.br||repositorio@instituicao.bropendoar:2018-07-18T15:06:21Biblioteca Digital de Teses e Dissertações da UNIGRANRIO - Universidade do Grande Rio (UNIGRANRIO)false |
dc.title.por.fl_str_mv |
Citotoxicidade de dois cimentos à base de silicato de cálcio |
title |
Citotoxicidade de dois cimentos à base de silicato de cálcio |
spellingShingle |
Citotoxicidade de dois cimentos à base de silicato de cálcio Estellita, Raffaella Sacchetti Odontologia Endodontia Citotoxicidade Materiais dentários Cimentos dentários Odontologia Endodontia |
title_short |
Citotoxicidade de dois cimentos à base de silicato de cálcio |
title_full |
Citotoxicidade de dois cimentos à base de silicato de cálcio |
title_fullStr |
Citotoxicidade de dois cimentos à base de silicato de cálcio |
title_full_unstemmed |
Citotoxicidade de dois cimentos à base de silicato de cálcio |
title_sort |
Citotoxicidade de dois cimentos à base de silicato de cálcio |
author |
Estellita, Raffaella Sacchetti |
author_facet |
Estellita, Raffaella Sacchetti |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Mendonça, Thais Accorsi |
dc.contributor.advisor2.fl_str_mv |
Moreira, Edson Jorge Lima |
dc.contributor.referee1.fl_str_mv |
Silva, Emmanoel João Nogueira Leal da |
dc.contributor.referee2.fl_str_mv |
Batista, Marcelo Mangelli Decnop |
dc.contributor.referee3.fl_str_mv |
Vieira, Victor Talarico Leal |
dc.contributor.author.fl_str_mv |
Estellita, Raffaella Sacchetti |
contributor_str_mv |
Mendonça, Thais Accorsi Moreira, Edson Jorge Lima Silva, Emmanoel João Nogueira Leal da Batista, Marcelo Mangelli Decnop Vieira, Victor Talarico Leal |
dc.subject.por.fl_str_mv |
Odontologia Endodontia Citotoxicidade Materiais dentários Cimentos dentários |
topic |
Odontologia Endodontia Citotoxicidade Materiais dentários Cimentos dentários Odontologia Endodontia |
dc.subject.cnpq.fl_str_mv |
Odontologia Endodontia |
description |
The aim of the present study was to evaluate MTA White and TheraCal LC Cytotoxicity. Control group used was treated with convencional cellular medium. The Cytotocity was determined by counting viable cells using the MTT colorimetric Assay. The extract of the materials was used undiluted and an experimental contact Time for 24 hours. Data from the MTT assay was performed using SPSS version 15.0 statistical software (SPSS, INC, Chicago,IL, USA). Data were tested for Normal distribution by the Kolmogorov-Smirnov test, and group comparisons were Analysed by one-way analysis of variance (ANOVA) followed by a Bonferroni post- test. The results showed statistically significant differences between the materials Testd ( P> 0.05 ) and between the materials and the control group ( P> 0.5 ). Its is Concluded that, within the limitations of the study, ther is difference between a MTA cytotoxicity where when compared to the TheraCal LC. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-03-08 |
dc.date.accessioned.fl_str_mv |
2018-07-18T15:06:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Estellita, Raffaella Sacchetti. Citotoxicidade de dois cimentos à base de silicato de cálcio. 2017. 35 f. Dissertação (Programa de Pós-Graduação em Odontologia) - Universidade do Grande Rio, Duque de Caxias. |
dc.identifier.uri.fl_str_mv |
http://localhost:8080/tede/handle/tede/231 |
identifier_str_mv |
Estellita, Raffaella Sacchetti. Citotoxicidade de dois cimentos à base de silicato de cálcio. 2017. 35 f. Dissertação (Programa de Pós-Graduação em Odontologia) - Universidade do Grande Rio, Duque de Caxias. |
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http://localhost:8080/tede/handle/tede/231 |
dc.language.iso.fl_str_mv |
por |
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por |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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Universidade do Grande Rio |
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Programa de Pós-Graduação em Odontologia |
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UNIGRANRIO |
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Brasil |
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Unigranrio::Odontologia |
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Universidade do Grande Rio |
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