Sexual transmission of American trypanosomiasis in humans : a new potential pandemic route for Chagas parasites

Detalhes bibliográficos
Autor(a) principal: Araújo, Perla Fabíola de
Data de Publicação: 2017
Outros Autores: Almeida, Adriana B., Pimentel, Carlos F., Silva, Adriano R., Sousa, Alessandro, Valente, Sebastião A., Valente, Vera C., Britto, Manuela M., Rosa, Ana C., Alves, Rozeneide M., Hagström, Luciana, Teixeira, Antonio Raimundo Lima Cruz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/30584
http://dx.doi.org/10.1590/0074-02760160538
Resumo: Background: the Trypanosoma cruzi infection endemic in Latin America has now spread to several countries across four continents; this endemic involves triatomine vector-free protists. We hypothesised that the sexual transmission of T. cruzi contributes to the ongoing spread of Chagas disease. Objectives: a short-term longitudinal study was conducted to evaluate this hypothesis. Methods: the study population comprised 109 subjects from four families, among whom 21 had been diagnosed with acute Chagas disease by direct parasitological analysis. Blood mononuclear cells and serum samples were obtained from each study subject once per year for three consecutive years. Enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence serological examinations were used to detect specific T. cruzi antibodies. Polymerase chain reaction of T. cruzi DNA revealed 188-nucleotide bands, which hybridised to a specific radiolabelled probe and were confirmed by cloning and sequencing. Results: three independent assessments at different time points revealed T. cruzi nuclear DNA footprints in 76% (83/109) of the study population with active infection. In contrast, the ELISA and indirect immunofluorescence assays detected the T. cruzi antibody in 28.4% (31/109) of the study samples. Moreover, the semen from 82.6% (19/23) of subjects people revealed harboured the 188- bp base pair T. cruzi footprint. Interestingly, the ejaculates of nuclear DNA-positive Chagas patient transmitted the T. cruzi upon peritoneal injection or infusion in the vagina of mice, and amastigotes were detected in the skeletal muscle, myocardium, vas deferens, and uterine tube. Main conclusions: T. cruzi infections can be transmitted from females or males to naïve mates through intercourse, and progeny showed discrepancies between the ratios of nuclear DNA footprints and specific antibody that can be explained by the tolerance attained during early embryo growth. Additional studies are needed to develop drugs to eradicate the infections. Additionally, the importance of a vigorous education, information, and communication program to prevent sexually transmitted Chagas disease in humans cannot be underemphasised.
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spelling Sexual transmission of American trypanosomiasis in humans : a new potential pandemic route for Chagas parasitesAmazôniaTrypanosoma cruziChagas, Doença deHumanosEpidemiologiaBackground: the Trypanosoma cruzi infection endemic in Latin America has now spread to several countries across four continents; this endemic involves triatomine vector-free protists. We hypothesised that the sexual transmission of T. cruzi contributes to the ongoing spread of Chagas disease. Objectives: a short-term longitudinal study was conducted to evaluate this hypothesis. Methods: the study population comprised 109 subjects from four families, among whom 21 had been diagnosed with acute Chagas disease by direct parasitological analysis. Blood mononuclear cells and serum samples were obtained from each study subject once per year for three consecutive years. Enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence serological examinations were used to detect specific T. cruzi antibodies. Polymerase chain reaction of T. cruzi DNA revealed 188-nucleotide bands, which hybridised to a specific radiolabelled probe and were confirmed by cloning and sequencing. Results: three independent assessments at different time points revealed T. cruzi nuclear DNA footprints in 76% (83/109) of the study population with active infection. In contrast, the ELISA and indirect immunofluorescence assays detected the T. cruzi antibody in 28.4% (31/109) of the study samples. Moreover, the semen from 82.6% (19/23) of subjects people revealed harboured the 188- bp base pair T. cruzi footprint. Interestingly, the ejaculates of nuclear DNA-positive Chagas patient transmitted the T. cruzi upon peritoneal injection or infusion in the vagina of mice, and amastigotes were detected in the skeletal muscle, myocardium, vas deferens, and uterine tube. Main conclusions: T. cruzi infections can be transmitted from females or males to naïve mates through intercourse, and progeny showed discrepancies between the ratios of nuclear DNA footprints and specific antibody that can be explained by the tolerance attained during early embryo growth. Additional studies are needed to develop drugs to eradicate the infections. Additionally, the importance of a vigorous education, information, and communication program to prevent sexually transmitted Chagas disease in humans cannot be underemphasised.Faculdade de Medicina (FMD)Instituto Oswaldo Cruz, Ministério da Saúde2018-01-04T19:13:15Z2018-01-04T19:13:15Z2017-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfARAUJO, Perla F et al. Sexual transmission of American trypanosomiasis in humans: a new potential pandemic route for Chagas parasites. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 112, n. 6, p. 437-446, jun. 2017. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000600437&lng=en&nrm=iso>. Acesso em: 22 fev. 2018. doi: http://dx.doi.org/10.1590/0074-02760160538.http://repositorio.unb.br/handle/10482/30584http://dx.doi.org/10.1590/0074-02760160538Memórias do Instituto Oswaldo Cruz - This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0). Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000600437&lng=en&nrm=iso. Acesso em: 22 fev. 2018.info:eu-repo/semantics/openAccessAraújo, Perla Fabíola deAlmeida, Adriana B.Pimentel, Carlos F.Silva, Adriano R.Sousa, AlessandroValente, Sebastião A.Valente, Vera C.Britto, Manuela M.Rosa, Ana C.Alves, Rozeneide M.Hagström, LucianaTeixeira, Antonio Raimundo Lima Cruzengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2023-08-25T19:49:28Zoai:repositorio.unb.br:10482/30584Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-08-25T19:49:28Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Sexual transmission of American trypanosomiasis in humans : a new potential pandemic route for Chagas parasites
title Sexual transmission of American trypanosomiasis in humans : a new potential pandemic route for Chagas parasites
spellingShingle Sexual transmission of American trypanosomiasis in humans : a new potential pandemic route for Chagas parasites
Araújo, Perla Fabíola de
Amazônia
Trypanosoma cruzi
Chagas, Doença de
Humanos
Epidemiologia
title_short Sexual transmission of American trypanosomiasis in humans : a new potential pandemic route for Chagas parasites
title_full Sexual transmission of American trypanosomiasis in humans : a new potential pandemic route for Chagas parasites
title_fullStr Sexual transmission of American trypanosomiasis in humans : a new potential pandemic route for Chagas parasites
title_full_unstemmed Sexual transmission of American trypanosomiasis in humans : a new potential pandemic route for Chagas parasites
title_sort Sexual transmission of American trypanosomiasis in humans : a new potential pandemic route for Chagas parasites
author Araújo, Perla Fabíola de
author_facet Araújo, Perla Fabíola de
Almeida, Adriana B.
Pimentel, Carlos F.
Silva, Adriano R.
Sousa, Alessandro
Valente, Sebastião A.
Valente, Vera C.
Britto, Manuela M.
Rosa, Ana C.
Alves, Rozeneide M.
Hagström, Luciana
Teixeira, Antonio Raimundo Lima Cruz
author_role author
author2 Almeida, Adriana B.
Pimentel, Carlos F.
Silva, Adriano R.
Sousa, Alessandro
Valente, Sebastião A.
Valente, Vera C.
Britto, Manuela M.
Rosa, Ana C.
Alves, Rozeneide M.
Hagström, Luciana
Teixeira, Antonio Raimundo Lima Cruz
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Araújo, Perla Fabíola de
Almeida, Adriana B.
Pimentel, Carlos F.
Silva, Adriano R.
Sousa, Alessandro
Valente, Sebastião A.
Valente, Vera C.
Britto, Manuela M.
Rosa, Ana C.
Alves, Rozeneide M.
Hagström, Luciana
Teixeira, Antonio Raimundo Lima Cruz
dc.subject.por.fl_str_mv Amazônia
Trypanosoma cruzi
Chagas, Doença de
Humanos
Epidemiologia
topic Amazônia
Trypanosoma cruzi
Chagas, Doença de
Humanos
Epidemiologia
description Background: the Trypanosoma cruzi infection endemic in Latin America has now spread to several countries across four continents; this endemic involves triatomine vector-free protists. We hypothesised that the sexual transmission of T. cruzi contributes to the ongoing spread of Chagas disease. Objectives: a short-term longitudinal study was conducted to evaluate this hypothesis. Methods: the study population comprised 109 subjects from four families, among whom 21 had been diagnosed with acute Chagas disease by direct parasitological analysis. Blood mononuclear cells and serum samples were obtained from each study subject once per year for three consecutive years. Enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence serological examinations were used to detect specific T. cruzi antibodies. Polymerase chain reaction of T. cruzi DNA revealed 188-nucleotide bands, which hybridised to a specific radiolabelled probe and were confirmed by cloning and sequencing. Results: three independent assessments at different time points revealed T. cruzi nuclear DNA footprints in 76% (83/109) of the study population with active infection. In contrast, the ELISA and indirect immunofluorescence assays detected the T. cruzi antibody in 28.4% (31/109) of the study samples. Moreover, the semen from 82.6% (19/23) of subjects people revealed harboured the 188- bp base pair T. cruzi footprint. Interestingly, the ejaculates of nuclear DNA-positive Chagas patient transmitted the T. cruzi upon peritoneal injection or infusion in the vagina of mice, and amastigotes were detected in the skeletal muscle, myocardium, vas deferens, and uterine tube. Main conclusions: T. cruzi infections can be transmitted from females or males to naïve mates through intercourse, and progeny showed discrepancies between the ratios of nuclear DNA footprints and specific antibody that can be explained by the tolerance attained during early embryo growth. Additional studies are needed to develop drugs to eradicate the infections. Additionally, the importance of a vigorous education, information, and communication program to prevent sexually transmitted Chagas disease in humans cannot be underemphasised.
publishDate 2017
dc.date.none.fl_str_mv 2017-06
2018-01-04T19:13:15Z
2018-01-04T19:13:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv ARAUJO, Perla F et al. Sexual transmission of American trypanosomiasis in humans: a new potential pandemic route for Chagas parasites. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 112, n. 6, p. 437-446, jun. 2017. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000600437&lng=en&nrm=iso>. Acesso em: 22 fev. 2018. doi: http://dx.doi.org/10.1590/0074-02760160538.
http://repositorio.unb.br/handle/10482/30584
http://dx.doi.org/10.1590/0074-02760160538
identifier_str_mv ARAUJO, Perla F et al. Sexual transmission of American trypanosomiasis in humans: a new potential pandemic route for Chagas parasites. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 112, n. 6, p. 437-446, jun. 2017. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000600437&lng=en&nrm=iso>. Acesso em: 22 fev. 2018. doi: http://dx.doi.org/10.1590/0074-02760160538.
url http://repositorio.unb.br/handle/10482/30584
http://dx.doi.org/10.1590/0074-02760160538
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dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
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reponame_str Repositório Institucional da UnB
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