Sensitivity of a vacuum aspiratory culture technique for diagnosis of localized cutaneous leishmaniasis in an endemic area of leishmania (Viannia) braziliensis transmission

Detalhes bibliográficos
Autor(a) principal: Romero, Gustavo Adolfo Sierra
Data de Publicação: 1999
Outros Autores: Sampaio, Raimunda Nonata Ribeiro, Macêdo, Vanize de Oliveira, Marsden, Philip Davis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/25497
https://dx.doi.org/10.1590/S0074-02761999000400014
Resumo: Sixty eight patients with localized cutaneous leishmaniasis from an area with Leishmania (Viannia) braziliensis transmission had cultures performed with a modified Marzochi´s vacuum aspiratory puncture technique to establish sensitivity and contamination rate with this new method. Overall sensitivity of three aspirates was 47.1%; (CI95% 39.4; 59.4) significantly greater than the sensitivity of a single one aspirate. Fungal contamination was observed in 6/204 (2.9%) inoculated culture tubes. We recommend that this useful technique should be adopted as routine for primary isolation of L. (V.) braziliensis from localized cutaneous ulcers.
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spelling Sensitivity of a vacuum aspiratory culture technique for diagnosis of localized cutaneous leishmaniasis in an endemic area of leishmania (Viannia) braziliensis transmissionLeishmania braziliensisContaminação fúngicaLeishmaniose cutâneaSixty eight patients with localized cutaneous leishmaniasis from an area with Leishmania (Viannia) braziliensis transmission had cultures performed with a modified Marzochi´s vacuum aspiratory puncture technique to establish sensitivity and contamination rate with this new method. Overall sensitivity of three aspirates was 47.1%; (CI95% 39.4; 59.4) significantly greater than the sensitivity of a single one aspirate. Fungal contamination was observed in 6/204 (2.9%) inoculated culture tubes. We recommend that this useful technique should be adopted as routine for primary isolation of L. (V.) braziliensis from localized cutaneous ulcers.Faculdade de Medicina (FMD)Instituto Oswaldo Cruz, Ministério da Saúde2017-12-07T04:28:53Z2017-12-07T04:28:53Z1999-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfROMERO, Gustavo Adolfo Sierra et al. Sensitivity of a vacuum aspiratory culture technique for diagnosis of localized cutaneous leishmaniasis in an endemic area of Leishmania (Viannia) braziliensis transmission. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 94, n. 4, p. 505-508, jul. 1999. DOI: https://doi.org/10.1590/S0074-02761999000400014. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761999000400014&lng=en&nrm=iso. Acesso em: 12 jan. 2021.http://repositorio.unb.br/handle/10482/25497https://dx.doi.org/10.1590/S0074-02761999000400014Memórias do Instituto Oswaldo Cruz - All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License (CC BY NC 4.0). Fonte: https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761999000400014&lng=en&tlng=en. Acesso em: 12 jan. 2021.info:eu-repo/semantics/openAccessRomero, Gustavo Adolfo SierraSampaio, Raimunda Nonata RibeiroMacêdo, Vanize de OliveiraMarsden, Philip Davisengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2023-08-25T19:46:40Zoai:repositorio.unb.br:10482/25497Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-08-25T19:46:40Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Sensitivity of a vacuum aspiratory culture technique for diagnosis of localized cutaneous leishmaniasis in an endemic area of leishmania (Viannia) braziliensis transmission
title Sensitivity of a vacuum aspiratory culture technique for diagnosis of localized cutaneous leishmaniasis in an endemic area of leishmania (Viannia) braziliensis transmission
spellingShingle Sensitivity of a vacuum aspiratory culture technique for diagnosis of localized cutaneous leishmaniasis in an endemic area of leishmania (Viannia) braziliensis transmission
Romero, Gustavo Adolfo Sierra
Leishmania braziliensis
Contaminação fúngica
Leishmaniose cutânea
title_short Sensitivity of a vacuum aspiratory culture technique for diagnosis of localized cutaneous leishmaniasis in an endemic area of leishmania (Viannia) braziliensis transmission
title_full Sensitivity of a vacuum aspiratory culture technique for diagnosis of localized cutaneous leishmaniasis in an endemic area of leishmania (Viannia) braziliensis transmission
title_fullStr Sensitivity of a vacuum aspiratory culture technique for diagnosis of localized cutaneous leishmaniasis in an endemic area of leishmania (Viannia) braziliensis transmission
title_full_unstemmed Sensitivity of a vacuum aspiratory culture technique for diagnosis of localized cutaneous leishmaniasis in an endemic area of leishmania (Viannia) braziliensis transmission
title_sort Sensitivity of a vacuum aspiratory culture technique for diagnosis of localized cutaneous leishmaniasis in an endemic area of leishmania (Viannia) braziliensis transmission
author Romero, Gustavo Adolfo Sierra
author_facet Romero, Gustavo Adolfo Sierra
Sampaio, Raimunda Nonata Ribeiro
Macêdo, Vanize de Oliveira
Marsden, Philip Davis
author_role author
author2 Sampaio, Raimunda Nonata Ribeiro
Macêdo, Vanize de Oliveira
Marsden, Philip Davis
author2_role author
author
author
dc.contributor.author.fl_str_mv Romero, Gustavo Adolfo Sierra
Sampaio, Raimunda Nonata Ribeiro
Macêdo, Vanize de Oliveira
Marsden, Philip Davis
dc.subject.por.fl_str_mv Leishmania braziliensis
Contaminação fúngica
Leishmaniose cutânea
topic Leishmania braziliensis
Contaminação fúngica
Leishmaniose cutânea
description Sixty eight patients with localized cutaneous leishmaniasis from an area with Leishmania (Viannia) braziliensis transmission had cultures performed with a modified Marzochi´s vacuum aspiratory puncture technique to establish sensitivity and contamination rate with this new method. Overall sensitivity of three aspirates was 47.1%; (CI95% 39.4; 59.4) significantly greater than the sensitivity of a single one aspirate. Fungal contamination was observed in 6/204 (2.9%) inoculated culture tubes. We recommend that this useful technique should be adopted as routine for primary isolation of L. (V.) braziliensis from localized cutaneous ulcers.
publishDate 1999
dc.date.none.fl_str_mv 1999-07
2017-12-07T04:28:53Z
2017-12-07T04:28:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv ROMERO, Gustavo Adolfo Sierra et al. Sensitivity of a vacuum aspiratory culture technique for diagnosis of localized cutaneous leishmaniasis in an endemic area of Leishmania (Viannia) braziliensis transmission. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 94, n. 4, p. 505-508, jul. 1999. DOI: https://doi.org/10.1590/S0074-02761999000400014. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761999000400014&lng=en&nrm=iso. Acesso em: 12 jan. 2021.
http://repositorio.unb.br/handle/10482/25497
https://dx.doi.org/10.1590/S0074-02761999000400014
identifier_str_mv ROMERO, Gustavo Adolfo Sierra et al. Sensitivity of a vacuum aspiratory culture technique for diagnosis of localized cutaneous leishmaniasis in an endemic area of Leishmania (Viannia) braziliensis transmission. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 94, n. 4, p. 505-508, jul. 1999. DOI: https://doi.org/10.1590/S0074-02761999000400014. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761999000400014&lng=en&nrm=iso. Acesso em: 12 jan. 2021.
url http://repositorio.unb.br/handle/10482/25497
https://dx.doi.org/10.1590/S0074-02761999000400014
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
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