The effect of carbon dioxide pneumoperitoneum on the healing colonic anastomosis in rats

• Autor(a) principal: Durães, Leonardo de Castro
• Data de Publicação: 2013
• Outros Autores: Farias, Igor Eduardo Caetano de, Morais, Pedro Henrique Alves de, Oliveira, Paulo Gonçalves de, Cavalcanti Neto, Florêncio Figueiredo, Lino Junior, Ruy de Souza, Feres, Omar, Sousa, João Batista de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UnB
• Texto Completo: http://repositorio.unb.br/handle/10482/28805; https://dx.doi.org/10.1590/S0102-86502013000900008
• topic: Pneumoperitônio; Dióxido de carbono; Anastomose; Cicatrização de feridas; Cólon (Anatomia) - cirurgia; Ratos

PURPOSE: To investigate the effects of pneumoperitoneum on colonic anastomosis healing. METHODS: Colonic anastomosis was performed in 120 rats divided into four groups: Group I - pneumoperitoneum before laparotomy, Group II - pneumoperitoneum after laparorrhaphy, Group III - pneumoperitoneum before laparotomy and after laparorrhaphy, Group IV - no pneumoperitoneum (control group). Pneumoperitoneum pressure was 5 mmHg. Animals were killed on the 3rd, 7th and 14th postoperative day. Hhistopathological features, anastomosis breaking strength, collagen histomorphometry and hydroxyproline concentration were assessed. RESULTS: Breaking strength between groups: (day 3, p=0.165; day 7, p=0.219; day 14, p=0.539). Histopathology revealed that group II had, on day 7, less infiltration of mononuclear cells (p=0.006), greater infiltration of polymorphonuclear cells (p=0.001) and greater necrosis (p=0.001); and on day 14, less fibrosis. Histomorphometry revealed a decrease in collagen in groups I and III (p<0.001) on day 7 and an increase in groups I and II on day 14 (p<0.001). Hydroxyproline concentration was similar for groups on days 3 (p=0.152), 7 (p=0.913) or 14 (p=0.981). CONCLUSION: Carbon dioxide does not impair the healing of colonic anastomosis in rats.