Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UnB |
Texto Completo: | http://repositorio.unb.br/handle/10482/29355 https://dx.doi.org/10.1590/S1415-47572014000100010 |
Resumo: | Brazilian goats are generally kept in small herds and extensive rearing systems, mainly in the northeastern region of the country. Despite production improvement in recent years, the lack of pedigree control has affected genetic progress. This study aimed to validate a panel of 16 microsatellites for parentage testing in locally adapted and commercial goats breeds raised in Brazil, as well as to compare its efficiency with the panel recommended by the Brazilian Ministry of Agriculture, Livestock and Supplies (MAPA) in 2004. The number of alleles and expected heterozygosity (He) per marker ranged from four to 18, and from 0.051 to 0.831, respectively. Using all markers, 100% of parentage cases of the validation dataset were resolved with a strict confidence level of 95%. The 16 microsatellites panel showed adequate exclusion power (99.99%) and identity accuracy (99.99%). Suggestions for improvement of the marker panel endorsed by MAPA are provided. |
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Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in BrazilAnimais - criaçãoMarcadores genéticosCaprinoBrazilian goats are generally kept in small herds and extensive rearing systems, mainly in the northeastern region of the country. Despite production improvement in recent years, the lack of pedigree control has affected genetic progress. This study aimed to validate a panel of 16 microsatellites for parentage testing in locally adapted and commercial goats breeds raised in Brazil, as well as to compare its efficiency with the panel recommended by the Brazilian Ministry of Agriculture, Livestock and Supplies (MAPA) in 2004. The number of alleles and expected heterozygosity (He) per marker ranged from four to 18, and from 0.051 to 0.831, respectively. Using all markers, 100% of parentage cases of the validation dataset were resolved with a strict confidence level of 95%. The 16 microsatellites panel showed adequate exclusion power (99.99%) and identity accuracy (99.99%). Suggestions for improvement of the marker panel endorsed by MAPA are provided.Faculdade de Agronomia e Medicina Veterinária (FAV)Sociedade Brasileira de Genética2017-12-07T05:08:44Z2017-12-07T05:08:44Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSILVA, Elizabete Cristina da et al. Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil. Genetics and Molecular Biology, Ribeirão Preto, v. 37, n. 1, p. 54-60, 2014. DOI: http://dx.doi.org/10.1590/S1415-47572014000100010. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100010&lng=en&nrm=iso. Acesso em: 20 nov. 2019.http://repositorio.unb.br/handle/10482/29355https://dx.doi.org/10.1590/S1415-47572014000100010Genetics and Molecular Biology - (CC BY NC) - All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License. Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100010&lng=en&tlng=en. Acesso em: 20 nov. 2019.info:eu-repo/semantics/openAccessSilva, Elizabete Cristina daPimentel, Concepta Margaret McManusGuimarães, Maria Pia Souza Lima Mattos de PaivaGouveia, Aurora M. G.Facó, OlivardoPimentel, Daniel M.Caetano, Alexandre RodriguesPaiva, Samuel Rezendeengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2023-09-01T19:34:28Zoai:repositorio.unb.br:10482/29355Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-09-01T19:34:28Repositório Institucional da UnB - Universidade de Brasília (UnB)false |
dc.title.none.fl_str_mv |
Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil |
title |
Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil |
spellingShingle |
Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil Silva, Elizabete Cristina da Animais - criação Marcadores genéticos Caprino |
title_short |
Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil |
title_full |
Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil |
title_fullStr |
Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil |
title_full_unstemmed |
Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil |
title_sort |
Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil |
author |
Silva, Elizabete Cristina da |
author_facet |
Silva, Elizabete Cristina da Pimentel, Concepta Margaret McManus Guimarães, Maria Pia Souza Lima Mattos de Paiva Gouveia, Aurora M. G. Facó, Olivardo Pimentel, Daniel M. Caetano, Alexandre Rodrigues Paiva, Samuel Rezende |
author_role |
author |
author2 |
Pimentel, Concepta Margaret McManus Guimarães, Maria Pia Souza Lima Mattos de Paiva Gouveia, Aurora M. G. Facó, Olivardo Pimentel, Daniel M. Caetano, Alexandre Rodrigues Paiva, Samuel Rezende |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Elizabete Cristina da Pimentel, Concepta Margaret McManus Guimarães, Maria Pia Souza Lima Mattos de Paiva Gouveia, Aurora M. G. Facó, Olivardo Pimentel, Daniel M. Caetano, Alexandre Rodrigues Paiva, Samuel Rezende |
dc.subject.por.fl_str_mv |
Animais - criação Marcadores genéticos Caprino |
topic |
Animais - criação Marcadores genéticos Caprino |
description |
Brazilian goats are generally kept in small herds and extensive rearing systems, mainly in the northeastern region of the country. Despite production improvement in recent years, the lack of pedigree control has affected genetic progress. This study aimed to validate a panel of 16 microsatellites for parentage testing in locally adapted and commercial goats breeds raised in Brazil, as well as to compare its efficiency with the panel recommended by the Brazilian Ministry of Agriculture, Livestock and Supplies (MAPA) in 2004. The number of alleles and expected heterozygosity (He) per marker ranged from four to 18, and from 0.051 to 0.831, respectively. Using all markers, 100% of parentage cases of the validation dataset were resolved with a strict confidence level of 95%. The 16 microsatellites panel showed adequate exclusion power (99.99%) and identity accuracy (99.99%). Suggestions for improvement of the marker panel endorsed by MAPA are provided. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2017-12-07T05:08:44Z 2017-12-07T05:08:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SILVA, Elizabete Cristina da et al. Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil. Genetics and Molecular Biology, Ribeirão Preto, v. 37, n. 1, p. 54-60, 2014. DOI: http://dx.doi.org/10.1590/S1415-47572014000100010. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100010&lng=en&nrm=iso. Acesso em: 20 nov. 2019. http://repositorio.unb.br/handle/10482/29355 https://dx.doi.org/10.1590/S1415-47572014000100010 |
identifier_str_mv |
SILVA, Elizabete Cristina da et al. Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil. Genetics and Molecular Biology, Ribeirão Preto, v. 37, n. 1, p. 54-60, 2014. DOI: http://dx.doi.org/10.1590/S1415-47572014000100010. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100010&lng=en&nrm=iso. Acesso em: 20 nov. 2019. |
url |
http://repositorio.unb.br/handle/10482/29355 https://dx.doi.org/10.1590/S1415-47572014000100010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
instname_str |
Universidade de Brasília (UnB) |
instacron_str |
UNB |
institution |
UNB |
reponame_str |
Repositório Institucional da UnB |
collection |
Repositório Institucional da UnB |
repository.name.fl_str_mv |
Repositório Institucional da UnB - Universidade de Brasília (UnB) |
repository.mail.fl_str_mv |
repositorio@unb.br |
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1814508288866254848 |