The circulation of human astrovirus genotypes in the Central West Region of Brazil

• Autor(a) principal: Silva, Paula Andreia
• Data de Publicação: 2009
• Outros Autores: Santos, Rodrigo Alessandro Tôgo, Costa, Paulo Sérgio Sucasas, Teixeira, José Marcus Sócrates, Giugliano, Loreny Gimenes, Andreasi, Márcia Sueli Assis, Leite, José Paulo Gagliardi, Schreier, Ecakart, Cardoso, Divina das Dôres de Paula
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UnB
• Texto Completo: http://repositorio.unb.br/handle/10482/27433; https://dx.doi.org/10.1590/S0074-02762009000400021
• topic: Astrovirus; Genotipagem; Sequenciamento; PCR; Brasil

Out of 1,588 faecal samples of children taken from three locations of the Central West Region of Brazil, 57 were positive for astroviruses (HAstVs) using reverse transcription-polymerase chain reaction (RT-PCR). They were genotyped by nested RT-PCR and/or genomic sequencing. HAstV-1 (42.8%), HAstV-2 (23.2%), HAstV-3 (3.6%), HAstV-4 (14.3%) and HAstVs -5, -6, -7 and -8 (1.8% each) were detected. In Goiânia and Campo Grande, HAstV-1 was the most frequently detected genotype while in Brasília (DF) it was HAstV-2. Shifts in the circulation of astrovirus genotypes were observed in DF and Campo Grande. All samples collected by rectal swabs were viral negative. The astrovirus genotypes were detected in all age groups and there was no correlation between genotype and age group.